Proliferation of mitochondria during the cell cycle of the human cell line (HL-60)

Using rhodamine 123 to stain mitochondria of the human cell line HL-60, we have followed their increase over the cell cycle by flow cytometry. A near-linear synthesis of mitochondrial mass was shown to occur over the cell cycle. A comparison with the cell's DNA synthesis pattern obtained by the same technique established a common time-base. The mitochondrial synthesis curve changes with culture age. As a control, thd dye was tested for its binding specificity and for its use to resolve mitochondria microscopically. Its stoichiometric range was established and, above 0.25 microgram/ml, it was shown to reduce growth rate and cell viability in culture.

[1]  M L Walsh,et al.  Localization of mitochondria in living cells with rhodamine 123. , 1980, Proceedings of the National Academy of Sciences of the United States of America.

[2]  C. B. Pickett,et al.  Quantitative isolation of liver mitochondria by zonal centrifugation. , 1978, Analytical biochemistry.

[3]  S. Collins,et al.  Continuous growth and differentiation of human myeloid leukaemic cells in suspension culture , 1977, Nature.

[4]  P. Nagley,et al.  Structure, synthesis and genetics of yeast mitochondrial DNA. , 1977, Advances in microbial physiology.

[5]  P. Slonimski,et al.  Ultrastructural Characterization of Mitochondria from a Yeast Mutant Sensitive to „Petite" Induction (uvs ϱ 72) , 1976 .

[6]  P. Slonimski,et al.  Packaging and Recombination of Mitochondrial DNA in Vegetatively Growing Yeast Cells , 1976 .

[7]  J. Steinkamp,et al.  Rapid staining methods for analysis of deoxyribonucleic acid and protein in mammalian cells. , 1976, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[8]  T. W. James,et al.  Parametric analysis of volume distributions of Schizosaccharomyces pombe and other cells. , 1975, Experimental cell research.

[9]  M. Burton,et al.  The mitochondrion of the flagellate, Polytomella agilis , 1974 .

[10]  J. Wells.  Mitochondrial DNA synthesis during the cell cycle of Saccharomyces cerevisiae. , 1974, Experimental cell research.

[11]  H P Hoffmann,et al.  Mitochondrion of Yeast: Ultrastructural Evidence for One Giant, Branched Organelle per Cell , 1973, Science.

[12]  C. J. Avers,et al.  Evidence of mitochondrial synchrony in synchronous cell cultures of yeast. , 1970, Biochemical and biophysical research communications.

[13]  D. Smith,et al.  The replication of mitochondrial DNA during the cell cycle in Saccharomyces lactis. , 1968, Proceedings of the National Academy of Sciences of the United States of America.