Functionalized Fullerene as an Artificial Vector for Transfection.

Materials. Dulbecco modified Eagle medium (DMEM) was purchased from Sigma, fetal bovine serum (FBS) from Equitech-Bio. The green fluorescent protein (GFP) vector plasmid (pGreen LANTERN-1) was purchased from Life Technologies and was propagated by the lysozyme-Triton procedure. Vector DNA was purified by the CsCl equilibrium density gradient centrifugation and phenol extractions in the presence of 1% sodium dodecyl sulfate (SDS). Final concentration of plasmid was determined by absorbance at 260 nm. Organic solvents were distilled and, when necessary, dried over molecular sieves 4Å before use.

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