Production and shedding of channel catfish virus (CCV) and thymidine kinase negative CCV in immersion exposed channel catfish fingerlings

The progression of channel catfish virus (CCV) and thymidine kinase negative recombinant CCV (CCVTK-) infections in immersion challenged channel catfish lctaluruspunctatus fingerlings was studied by plaque assay and quantitative polymerase chain reaction (QT-PCR). lnfectious CCV was isolated from Day 2 through Day 8 in skin and blood and through Day 10 P1 in posterior kidney and gills. Infectious CCVTKwas detected from Day 2 through Day 6 P1 in skin and blood and through Day 8 PI in posterior kidney and gills. The kinetics of both infections were similar until Day 5 PI, after which virus concentrations were higher in CCV infected fish than in those exposed to CCVTKBoth viruses were detected in water; however, the CCV concentration was higher than CCVTKduring all time periods and was detected on Days 2 through 5 PI, whereas CCVTKwas detected on Days 3 through 5 PI. The QT-PCR showed higher amounts of viral DNA in the skin and water at early and late periods in infection in CCV challenged groups as opposed to CCVTK groups. This study indicates that the kinetics of CCV and CCVTKinfections are similar but CCVTK infections do not persist as long and have reduced shedding ability.

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