Transaminations with isopropyl amine: equilibrium displacement with yeast alcohol dehydrogenase coupled to in situ cofactor regeneration.

Enantiopure chiral amines synthesis using omega-transaminases is hindered by an unfavourable equilibrium, but when using isopropylamine as the amine donor the equilibrium can be completely displaced by using a specific dehydrogenase in situ for removal of formed acetone.

[1]  Wolfgang Kroutil,et al.  Asymmetric Synthesis of Optically Pure Pharmacologically Relevant Amines Employing ω‐Transaminases , 2008 .

[2]  Uwe T. Bornscheuer,et al.  Biocatalytic Routes to Optically Active Amines , 2009 .

[3]  Desiree Pressnitz,et al.  Deracemization of mexiletine biocatalyzed by omega-transaminases. , 2009, Organic letters.

[4]  D. Peričin,et al.  The three zinc-containing alcohol dehydrogenases from baker's yeast, Saccharomyces cerevisiae. , 2002, FEMS yeast research.

[5]  W. Kroutil,et al.  Synthesis of Optically Active Amines Employing Recombinant ω‐Transaminases in E. coli Cells , 2010 .

[6]  W. Kroutil,et al.  Deracemisation of α‐Chiral Primary Amines by a One‐Pot, Two‐Step Cascade Reaction Catalysed by ω‐Transaminases , 2009 .

[7]  Jeffrey C. Moore,et al.  Rapid screening and scale-up of transaminase catalysed reactions. , 2009, Organic & biomolecular chemistry.

[8]  Karen Robins,et al.  Efficient Asymmetric Synthesis of Chiral Amines by Combining Transaminase and Pyruvate Decarboxylase , 2008, Chembiochem : a European journal of chemical biology.

[9]  Byung Gee Kim,et al.  Asymmetric synthesis of chiral amines with ω‐transaminase , 1999 .

[10]  I. Fotheringham,et al.  Novel biosynthetic approaches to the production of unnatural amino acids using transaminases. , 1998, Trends in biotechnology.

[11]  Nicholas J Turner,et al.  Efficient kinetic resolution of racemic amines using a transaminase in combination with an amino acid oxidase. , 2009, Chemical communications.

[12]  Karen Robins,et al.  Rapid and sensitive kinetic assay for characterization of omega-transaminases. , 2009, Analytical chemistry.

[13]  G. Guebitz,et al.  Formal asymmetric biocatalytic reductive amination. , 2008, Angewandte Chemie.

[14]  Byung Gee Kim,et al.  Kinetic modeling of ω‐transamination for enzymatic kinetic resolution of α‐methylbenzylamine , 1998 .