Prevalence and pathogenicity of Cryptosporidium andersoni in one herd of beef cattle.

Over a 35-week period from January to July 2002, a breed of Hereford beef cattle (H) and their hybrids were monitored. Five hundred and ninety-nine individual fecal samples from calves and 96 samples from their mothers were examined. First excretion of Cryptosporidium andersoni oocysts in calves was found in the 9th week after the start of calving (a calf 63-day old). The prevalence of C. andersoni in calves ranged from 11.1 to 92.9% depending on age. The mean prevalence in their mothers was found to be 43.8%. The size of oocysts was 8.48 +/- 0.78 x 6.41 +/- 0.59 microm. Infection intensity in calves ranged from 32 000 to 4 375 000 oocysts per gram (OPG) and in mothers from 78 000 to 2 552 000 OPG. Three cases of abomasal cryptosporidiosis slaughtered at the age of 81, 157 and 236 days were examined histologically and ultrastructurally [transmission electron microscopy (TEM) and scanning electron microscopy (SEM)]. Cryptosporidium infection of the abomasum was located in the upper half of the mucosal glands in the plicae spirales of the fundus, corpus and near the ostium omasoabomasicum. Cryptosporidia were not located in the glandular epithelium of the pars pylorica in the abomasum minimally 10 cm from pylorus. Histopathological changes in the site of cryptosporidial infection in the abomasum had a non-inflammatory character and included distinctive dilatation of infected parts of the glands with atrophy and metaplasia of the glandular epithelial cells, goblet cell activation and mucus hyperproduction. The TEM revealed a relatively small number of Cryptosporidium life cycle stages attached to glandular epithelial cells. In SEM the inner mucosal abomasal surface appeared swollen but was never infected by cryptosporidia.

[1]  M. Iseki,et al.  Infectivity ofCryptosporidium muris (strain RN 66) in various laboratory animals , 2004, Parasitology Research.

[2]  M. Olson,et al.  Prevalence and infection pattern of naturally acquired giardiasis and cryptosporidiosis in range beef calves and their dams. , 2003, Veterinary parasitology.

[3]  B. Meloni,et al.  Successful in vitro cultivation of Cryptosporidium andersoni: evidence for the existence of novel extracellular stages in the life cycle and implications for the classification of Cryptosporidium. , 2002, International journal for parasitology.

[4]  V. Bille-Hansen,et al.  Cryptosporidium andersoni from a Danish cattle herd: identification and preliminary characterisation. , 2002, Veterinary parasitology.

[5]  J. Markovits,et al.  Cryptosporidium muris-like Infection in Stomach of Cynomolgus Monkeys (Macaca fascicularis) , 2002, Veterinary pathology.

[6]  M. Nikolausz,et al.  MORPHOLOGIC, HOST SPECIFICITY, AND GENETIC CHARACTERIZATION OF A EUROPEAN CRYPTOSPORIDIUM ANDERSONI ISOLATE , 2000, The Journal of parasitology.

[7]  T. Graczyk,et al.  Prevalence of Cryptosporidium, Giardia and Eimeria infections in post-weaned and adult cattle on three Maryland farms. , 2000, Veterinary parasitology.

[8]  H. Mohammed,et al.  Prevalence of Giardia sp. Cryptosporidium parvum and Cryptosporidium andersoni (syn. C. muris) [correction of Cryptosporidium parvum and Cryptosporidium muris (C. andersoni)] in 109 dairy herds in five counties of southeastern New York. , 2000, Veterinary parasitology.

[9]  D. Lindsay,et al.  Cryptosporidium andersoni n. sp. (Apicomplexa: Cryptosporiidae) from Cattle, Bos taurus , 2000, The Journal of eukaryotic microbiology.

[10]  M. Taylor,et al.  The pathogenesis of experimental infections of Cryptosporidium muris (strain RN 66) in outbred nude mice. , 1999, Veterinary parasitology.

[11]  D. Modrý,et al.  Infectivity of Cryptosporidium muris isolated from cattle. , 1998, Veterinary parasitology.

[12]  S. Gennari,et al.  Cryptosporidium muris in dairy cattle in Brazil. , 1997, Veterinary parasitology.

[13]  J. Rose,et al.  Development of a PCR protocol for sensitive detection of Cryptosporidium oocysts in water samples , 1995, Applied and environmental microbiology.

[14]  E. Esteban,et al.  Cryptosporidium muris: prevalence, persistency, and detrimental effect on milk production in a drylot dairy. , 1995, Journal of dairy science.

[15]  Y. Aydin,et al.  Natural Cryptosporidium muris infection of the stomach in laboratory mice. , 1994, Veterinary parasitology.

[16]  A. Pospischil,et al.  Abomasal cryptosporidiosis in mountain gazelles , 1987, Veterinary Record.

[17]  B. C. Anderson,et al.  Abomasal Cryptosporidiosis in Cattle , 1987, Veterinary pathology.

[18]  S J Upton,et al.  The species of Cryptosporidium (Apicomplexa: Cryptosporidiidae) infecting mammals. , 1985, The Journal of parasitology.

[19]  J. Vítovec,et al.  Differential staining of cryptosporidia by aniline-carbol-methyl violet and tartrazine in smears from feces and scrapings of intestinal mucosa. , 1985, Folia parasitologica.

[20]  Henriksen Sa,et al.  Staining of Cryptosporidia by a Modified Ziehl-Neelsen Technique , 1981, Acta Veterinaria Scandinavica.

[21]  E. E. Tyzzer.  A sporozoan found in the peptic glands of the common mouse , 1907 .