Preparation and properties of tannic acid cross-linked collagen scaffold and its application in wound healing.

A biodurable porous scaffold of collagen with good biocompatibility and enhanced wound healing potential is prepared through casting technique using tannic acid (TA) as crosslinker. The morphological analysis of the tannic acid cross-linked collagen scaffold (TCCs) distinctively shows scaly interlinks with large pores. The enzymatic stability of the scaffold is characterized in vitro to detail the role of TA in stabilization of collagen matrix against collagenolytic degradation. TCCs shows more stability (>54%) against collagenase than that of the collagen scaffolds (Cs). The attenuated total reflectance Fourier transform infrared analysis of the TCCs confirms the noncovalent interaction between collagen and TA. The biocompatibility of the scaffold (TCCs) in vitro has been established using 3T3 fibroblasts. Therapeutic and wound healing potential of the TCCs has been studied in vivo using excision wound model in rats. The results clearly indicates that the TCCs has greater and significant effect in wound closure and increased the wound healing rate compared with native Cs. This biocompatible and biodurable scaffold may find broad applications in the tissue engineering and drug delivery applications.

[1]  Karen J L Burg,et al.  Tannic Acid Cross-linked Collagen Scaffolds and Their Anti-cancer Potential in a Tissue Engineered Breast Implant , 2012, Journal of biomaterials science. Polymer edition.

[2]  J. Czernuszka,et al.  Macrophage-mediated degradation of crosslinked collagen scaffolds. , 2011, Acta biomaterialia.

[3]  A. Potapovich,et al.  The promise of plant polyphenols as the golden standard skin anti-inflammatory agents. , 2010, Current drug metabolism.

[4]  F. Mi,et al.  Novel technology for the preparation of self-assembled catechin/gelatin nanoparticles and their characterization. , 2010, Journal of agricultural and food chemistry.

[5]  Jean Chmielewski,et al.  Higher-order assembly of collagen peptides into nano- and microscale materials. , 2010, Biochemistry.

[6]  A. Motta,et al.  Evaluation of composition and crosslinking effects on collagen-based composite constructs. , 2010, Acta biomaterialia.

[7]  H. Burt,et al.  The inhibition of collagenase induced degradation of collagen by the galloyl-containing polyphenols tannic acid, epigallocatechin gallate and epicatechin gallate , 2010, Journal of materials science. Materials in medicine.

[8]  R. G. Paul,et al.  The influence of a natural cross-linking agent (Myrica rubra) on the properties of extruded collagen fibres for tissue engineering applications , 2010 .

[9]  J. Werkmeister,et al.  Collagens as biomaterials , 2009, Journal of materials science. Materials in medicine.

[10]  Dimitrios I Zeugolis,et al.  Cross-linking of extruded collagen fibers--a biomimetic three-dimensional scaffold for tissue engineering applications. , 2009, Journal of biomedical materials research. Part A.

[11]  Chun-Hsu Yao,et al.  Asymmetric chitosan membrane containing collagen I nanospheres for skin tissue engineering. , 2009, Biomacromolecules.

[12]  Christopher B. Stabler,et al.  Polyphenol-stabilized tubular elastin scaffolds for tissue engineered vascular grafts. , 2009, Tissue engineering. Part A.

[13]  Sheng-Shou Hu,et al.  Intramyocardial injection of tannic acid attenuates postinfarction remodeling: a novel approach to stabilize the breaking extracellular matrix. , 2009, The Journal of thoracic and cardiovascular surgery.

[14]  M. Yamauchi,et al.  Effects of Natural Cross-Linkers on the Stability of Dentin Collagen and the Inhibition of Root Caries in vitro , 2008, Caries Research.

[15]  B. Nair,et al.  Role of green tea polyphenols in the inhibition of collagenolytic activity by collagenase. , 2007, International journal of biological macromolecules.

[16]  A. Kocot,et al.  Tannic acid-stabilized pericardium tissue: IR spectroscopy, atomic force microscopy, and dielectric spectroscopy investigations. , 2006, Journal of biomedical materials research. Part A.

[17]  Kela Liu,et al.  Ellagic and tannic acids protect newly synthesized elastic fibers from premature enzymatic degradation in dermal fibroblast cultures. , 2006, The Journal of investigative dermatology.

[18]  T. Ramasami,et al.  Stabilization of collagen using plant polyphenol: role of catechin. , 2005, International journal of biological macromolecules.

[19]  D. Mantovani,et al.  Preparation of ready-to-use, stockable and reconstituted collagen. , 2005, Macromolecular bioscience.

[20]  D. Simionescu,et al.  Elastin stabilization in cardiovascular implants: improved resistance to enzymatic degradation by treatment with tannic acid. , 2004, Biomaterials.

[21]  P. K. Sehgal,et al.  Alpha-crystallin-incorporated collagen matrices as an aid for dermal wound healing. , 2004, Journal of biomedical materials research. Part B, Applied biomaterials.

[22]  R. A. Hancock,et al.  Structure-activity relationships in the hydrophobic interactions of polyphenols with cellulose and collagen. , 2003, Biopolymers.

[23]  R. A. Hancock,et al.  Use of DSC to detect the heterogeneity of hydrothermal stability in the polyphenol-treated collagen matrix. , 2003, Journal of agricultural and food chemistry.

[24]  R. Kreis,et al.  Transaminase and alkaline phosphatase activity in the serum of burn patients treated with highly purified tannic acid. , 2002, Burns : journal of the International Society for Burn Injuries.

[25]  H. Lehnert,et al.  Expression of matrix-metalloproteinases and their inhibitors in the wounds of diabetic and non-diabetic patients , 2002, Diabetologia.

[26]  I. K. Cohen,et al.  MMP-8 is the predominant collagenase in healing wounds and nonhealing ulcers. , 1999, The Journal of surgical research.

[27]  W. Friess,et al.  Collagen--biomaterial for drug delivery. , 1998, European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V.

[28]  R. Kreis,et al.  Cross-linking of dermal sheep collagen with tannic acid. , 1997, Biomaterials.

[29]  F. Grinnell,et al.  Wound fluid from chronic leg ulcers contains elevated levels of metalloproteinases MMP-2 and MMP-9. , 1993, The Journal of investigative dermatology.