Nernst-ping-pong model for evaluating the effects of the substrate concentration and anode potential on the kinetic characteristics of bioanode.

Understanding the electron-transfer mechanism and kinetic characteristics of bioanodes is greatly significant to enhance the electron-generating efficiencies in bioelectrochemical systems (BESs). A Nernst-ping-pong model is proposed here to investigate the kinetics and biochemical processes of bioanodes in a microbial electrolysis cell. This model can accurately describe the effects of the substrate (including substrate inhibition) and the anode potential on the current of bioanodes. Results show that the half-wave potential positively shifts as the substrate concentration increases, indicating that the rate-determining steps of anodic processes change from substrate oxidation to intracellular electron transport reaction. The anode potential has negligible effects on the enzymatic catalysis of anodic microbes in the range of -0.25 V to +0.1 V vs. a saturated calomel electrode. It turns out that to reduce the anodic energy loss caused by overpotential, higher substrate concentrations are preferred, if the substrate do not significantly and adversely affect the output current.

[1]  Hong Liu,et al.  Electrochemically assisted microbial production of hydrogen from acetate. , 2005, Environmental science & technology.

[2]  D. R. Bond,et al.  Shewanella secretes flavins that mediate extracellular electron transfer , 2008, Proceedings of the National Academy of Sciences.

[3]  Derek R. Lovley,et al.  Application of cyclic voltammetry to investigate enhanced catalytic current generation by biofilm-modified anodes of Geobacter sulfurreducens strain DL1 vs. variant strain KN400 , 2011 .

[4]  H. Hamelers,et al.  Butler-Volmer-Monod model for describing bio-anode polarization curves. , 2011, Bioresource technology.

[5]  J. Savéant,et al.  Kinetic control by the substrate and the cosubstrate in electrochemically monitored redox enzymatic immobilized systems. Catalytic responses in cyclic voltammetry and steady state techniques , 2002 .

[6]  Huan Liu,et al.  Electrochemical characterization of a single electricity-producing bacterial cell of Shewanella by using optical tweezers. , 2010, Angewandte Chemie.

[7]  S. Elliott,et al.  Electrochemical interrogations of the Mtr cytochromes from Shewanella: opening a potential window , 2008, JBIC Journal of Biological Inorganic Chemistry.

[8]  F. Wiertz,et al.  Amicyanin transfers electrons from methylamine dehydrogenase to cytochrome c-551i via a ping-pong mechanism, not a ternary complex. , 2010, Journal of the American Chemical Society.

[9]  Abraham Esteve-Núñez,et al.  C-type cytochromes wire electricity-producing bacteria to electrodes. , 2008, Angewandte Chemie.

[10]  N. Mano,et al.  Effect of substrate inhibition and cooperativity on the electrochemical responses of glucose dehydrogenase. Kinetic characterization of wild and mutant types. , 2011, Journal of the American Chemical Society.

[11]  Yan Xiang,et al.  Enhancement of hydrogen production in a single chamber microbial electrolysis cell through anode arrangement optimization. , 2011, Bioresource technology.

[12]  A. McEwan,et al.  A mechanistic and electrochemical study of the interaction between dimethyl sulfide dehydrogenase and its electron transfer partner cytochrome c2 , 2008, JBIC Journal of Biological Inorganic Chemistry.

[13]  D. Lovley,et al.  Novel Mode of Microbial Energy Metabolism: Organic Carbon Oxidation Coupled to Dissimilatory Reduction of Iron or Manganese , 1988, Applied and environmental microbiology.

[14]  R. P. Pinto,et al.  A two-population bio-electrochemical model of a microbial fuel cell. , 2010, Bioresource technology.

[15]  Wang,et al.  A method of graphically analyzing substrate-inhibition kinetics. , 1999, Biotechnology and bioengineering.

[16]  Shoichi Matsuda,et al.  Redox-responsive switching in bacterial respiratory pathways involving extracellular electron transfer. , 2010, ChemSusChem.

[17]  Hyung-Sool Lee,et al.  Effects of substrate diffusion and anode potential on kinetic parameters for anode-respiring bacteria. , 2009, Environmental science & technology.

[18]  Byung Hong Kim,et al.  Direct electrode reaction of Fe(III)-reducing bacterium, Shewanella putrefaciens , 1999 .

[19]  B. Tartakovsky,et al.  Multi-population model of a microbial electrolysis cell. , 2011, Environmental science & technology.

[20]  Stefano Freguia,et al.  Microbial fuel cells: methodology and technology. , 2006, Environmental science & technology.

[21]  S. Prodanovic.  Microbial Fuel Cell , 2011 .

[22]  Derek R. Lovley,et al.  Biofilm and Nanowire Production Leads to Increased Current in Geobacter sulfurreducens Fuel Cells , 2006, Applied and Environmental Microbiology.

[23]  Duu-Jong Lee,et al.  Reduced internal resistance of microbial electrolysis cell (MEC) as factors of configuration and stuffing with granular activated carbon , 2010 .

[24]  D. Lovley.  The microbe electric: conversion of organic matter to electricity. , 2008, Current opinion in biotechnology.

[25]  Prathap Parameswaran,et al.  Kinetic experiments for evaluating the Nernst-Monod model for anode-respiring bacteria (ARB) in a biofilm anode. , 2008, Environmental science & technology.

[26]  J. Savéant,et al.  Electrochemistry of immobilized redox enzymes: kinetic characteristics of NADH oxidation catalysis at diaphorase monolayers affinity immobilized on electrodes. , 2006, Journal of the American Chemical Society.

[27]  J. C. Thrash,et al.  Evidence for Direct Electron Transfer by a Gram-Positive Bacterium Isolated from a Microbial Fuel Cell , 2011, Applied and Environmental Microbiology.

[28]  J. Savéant.  Enzymatic Catalysis of Electrochemical Reactions , 2006 .

[29]  J. Lloyd,et al.  Secretion of Flavins by Shewanella Species and Their Role in Extracellular Electron Transfer , 2007, Applied and Environmental Microbiology.

[30]  Bruce E Rittmann,et al.  Conduction‐based modeling of the biofilm anode of a microbial fuel cell , 2007, Biotechnology and bioengineering.

[31]  H. Hamelers,et al.  Principle and perspectives of hydrogen production through biocatalyzed electrolysis , 2006 .

[32]  Derek R. Lovley,et al.  Cyclic voltammetry of biofilms of wild type and mutant Geobacter sulfurreducens on fuel cell anodes indicates possible roles of OmcB, OmcZ, type IV pili, and protons in extracellular electron transfer , 2009 .