Photothermal quantitative phase imaging of living cells with nanoparticles utilizing a cost-efficient setup

We explored photothermal quantitative phase imaging (PTQPI) of living cells with functionalized nanoparticles (NPs) utilizing a cost-efficient setup based on a cell culture microscope. The excitation light was modulated by a mechanical chopper wheel with low frequencies. Quantitative phase imaging (QPI) was performed with Michelson interferometer-based off-axis digital holographic microscopy and a standard industrial camera. We present results from PTQPI observations on breast cancer cells that were incubated with functionalized gold NPs binding to the epidermal growth factor receptor. Moreover, QPI was used to quantify the impact of the NPs and the low frequency light excitation on cell morphology and viability.

[1]  Gabriel Popescu,et al.  Fourier phase microscopy for investigation of biological structures and dynamics. , 2004, Optics letters.

[2]  Natan T Shaked,et al.  Reflective interferometric chamber for quantitative phase imaging of biological sample dynamics. , 2010, Journal of biomedical optics.

[3]  E. Cuche,et al.  Simultaneous amplitude-contrast and quantitative phase-contrast microscopy by numerical reconstruction of Fresnel off-axis holograms. , 1999, Applied optics.

[4]  Gabriel Popescu,et al.  Hilbert phase microscopy for investigating fast dynamics in transparent systems. , 2005, Optics letters.

[5]  D. Dirksen,et al.  Autofocusing in digital holographic phase contrast microscopy on pure phase objects for live cell imaging. , 2008, Applied optics.

[6]  Daniel Carl,et al.  Modular digital holographic microscopy system for marker free quantitative phase contrast imaging of living cells , 2006, SPIE Photonics Europe.

[7]  O. Haeberlé,et al.  High-resolution three-dimensional tomographic diffractive microscopy of transparent inorganic and biological samples. , 2009, Optics letters.

[8]  C. Fang-Yen,et al.  Tomographic phase microscopy , 2008, Nature Methods.

[9]  R. Dasari,et al.  Diffraction phase microscopy for quantifying cell structure and dynamics. , 2006, Optics letters.

[10]  Jong Chul Ye,et al.  Self-reference quantitative phase microscopy for microfluidic devices. , 2010, Optics letters.

[11]  Chun-Min Lo,et al.  High-resolution quantitative phase-contrast microscopy by digital holography. , 2005, Optics express.

[12]  Natan T Shaked,et al.  Dual-interference-channel quantitative-phase microscopy of live cell dynamics. , 2009, Optics letters.

[13]  Itay Barnea,et al.  Detection and controlled depletion of cancer cells using photothermal phase microscopy , 2015, Journal of biophotonics.

[14]  Björn Kemper,et al.  Simplified approach for quantitative digital holographic phase contrast imaging of living cells. , 2011, Journal of biomedical optics.

[15]  B. Wattellier,et al.  Quadriwave lateral shearing interferometry for quantitative phase microscopy of living cells. , 2009, Optics express.

[16]  Steffi Ketelhut,et al.  Prospects and challenges of quantitative phase imaging in tumor cell biology , 2016, SPIE BiOS.

[17]  Steffi Ketelhut,et al.  Continuous morphology and growth monitoring of different cell types in a single culture using quantitative phase microscopy , 2015, Optical Metrology.

[18]  Patrik Langehanenberg,et al.  Determination of the integral refractive index of cells in suspension by digital holographic phase contrast microscopy , 2008, SPIE Photonics Europe.

[19]  Steffi Ketelhut,et al.  Enhanced quantitative phase imaging in self-interference digital holographic microscopy using an electrically focus tunable lens. , 2014, Biomedical optics express.

[20]  E. Cuche,et al.  Digital holographic microscopy: a noninvasive contrast imaging technique allowing quantitative visualization of living cells with subwavelength axial accuracy. , 2005, Optics letters.

[21]  B. Kemper,et al.  Digital holographic microscopy for live cell applications and technical inspection. , 2008, Applied optics.

[22]  Daniel Carl,et al.  Investigation of living pancreas tumor cells by digital holographic microscopy. , 2006, Journal of biomedical optics.

[23]  Daniel Carl,et al.  Parameter-optimized digital holographic microscope for high-resolution living-cell analysis. , 2004, Applied optics.

[24]  Huafeng Ding,et al.  Instantaneous Spatial Light Interference Microscopy. , 2010, Optics express.

[25]  Steffi Ketelhut,et al.  Multimodal Quantitative Phase Imaging with Digital Holographic Microscopy Accurately Assesses Intestinal Inflammation and Epithelial Wound Healing. , 2016, Journal of visualized experiments : JoVE.

[26]  Tomasz Kozacki,et al.  Reconstruction of refractive-index distribution in off-axis digital holography optical diffraction tomographic system. , 2009, Optics express.

[27]  Steffi Ketelhut,et al.  Quantitative Stain-Free and Continuous Multimodal Monitoring of Wound Healing In Vitro with Digital Holographic Microscopy , 2014, PloS one.

[28]  Natan T. Shaked,et al.  Wide-field interferometric phase microscopy with molecular specificity using plasmonic nanoparticles , 2013, Journal of biomedical optics.