Bmc Molecular Biology Selection of Housekeeping Genes for Gene Expression Studies in Larvae from Flatfish Using Real-time Pcr

Background: Flatfish metamorphosis involves major physiological and morphological changes. Due to its importance in aquaculture and as a model for developmental studies, some gene expression studies have focused on the understanding of this process using quantitative real-time PCR (qRT-PCR) technique. Therefore, adequate reference genes for accurate normalization are required.

[1]  Rainer Froese,et al.  FishBase. World Wide Web electronic publication. , 2014 .

[2]  Manuel Manchado,et al.  Thyroid hormones down-regulate thyrotropin beta subunit and thyroglobulin during metamorphosis in the flatfish Senegalese sole (Solea senegalensis Kaup). , 2008, General and comparative endocrinology.

[3]  Manuel Manchado,et al.  Differential gene expression and dependence on thyroid hormones of two glyceraldehyde-3-phosphate dehydrogenases in the flatfish Senegalese sole (Solea senegalensis Kaup). , 2007, Gene.

[4]  Manuel Manchado,et al.  Comparative sequence analysis of the complete set of 40S ribosomal proteins in the Senegalese sole (Solea senegalensis Kaup) and Atlantic halibut (Hippoglossus hippoglossus L.) (Teleostei: Pleuronectiformes): phylogeny and tissue- and development-specific expression , 2007, BMC Evolutionary Biology.

[5]  Jianying Hu,et al.  Development and validation of endogenous reference genes for expression profiling of medaka (Oryzias latipes) exposed to endocrine disrupting chemicals by quantitative real-time RT-PCR. , 2007, Toxicological sciences : an official journal of the Society of Toxicology.

[6]  I. Johnston,et al.  Profiling of maternal and developmental-stage specific mRNA transcripts in Atlantic halibut Hippoglossus hippoglossus. , 2007, Gene.

[7]  Manuel Manchado,et al.  Insulin-like growth factors I and II in the sole Solea senegalensis: cDNA cloning and quantitation of gene expression in tissues and during larval development. , 2006, General and comparative endocrinology.

[8]  D. Power,et al.  Molecular, cellular and histological changes in skin from a larval to an adult phenotype during bony fish metamorphosis , 2006, Cell and Tissue Research.

[9]  Steven Maere,et al.  The gain and loss of genes during 600 million years of vertebrate evolution , 2006, Genome Biology.

[10]  D. Power,et al.  Thyroid and pituitary gland development from hatching through metamorphosis of a teleost flatfish, the Atlantic halibut , 2005, Anatomy and Embryology.

[11]  P. Olsvik,et al.  Evaluation of potential reference genes in real-time RT-PCR studies of Atlantic salmon , 2005, BMC Molecular Biology.

[12]  S A Bustin,et al.  Quantitative real-time RT-PCR--a perspective. , 2005, Journal of molecular endocrinology.

[13]  M. Tagawa,et al.  Production of symmetrical flatfish by controlling the timing of thyroid hormone treatment in spotted halibut Verasper variegatus. , 2005, General and comparative endocrinology.

[14]  D. Power,et al.  Staging of Atlantic halibut (Hippoglossus hippoglossus L.) from first feeding through metamorphosis, including cranial ossification independent of eye migration , 2004 .

[15]  Tania Nolan,et al.  Pitfalls of quantitative real-time reverse-transcription polymerase chain reaction. , 2004, Journal of biomolecular techniques : JBT.

[16]  Claus Lindbjerg Andersen,et al.  Normalization of Real-Time Quantitative Reverse Transcription-PCR Data: A Model-Based Variance Estimation Approach to Identify Genes Suited for Normalization, Applied to Bladder and Colon Cancer Data Sets , 2004, Cancer Research.

[17]  P. Saussoy,et al.  Evaluation of real-time PCR data. , 2004, Journal of biological regulators and homeostatic agents.

[18]  M. Pfaffl,et al.  Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper – Excel-based tool using pair-wise correlations , 2004, Biotechnology Letters.

[19]  Alfred Pingoud,et al.  Real‐Time Polymerase Chain Reaction , 2003, Chembiochem : a European journal of chemical biology.

[20]  Zhanjiang Liu,et al.  Translational machinery of channel catfish: II. Complementary DNA and expression of the complete set of 47 60S ribosomal proteins. , 2003, Gene.

[21]  F. Speleman,et al.  Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes , 2002, Genome Biology.

[22]  Zhanjiang Liu,et al.  Translational machinery of channel catfish: I. A transcriptomic approach to the analysis of 32 40S ribosomal protein genes and their expression. , 2002, Gene.

[23]  J. Cañavate,et al.  Growth and physiological changes during metamorphosis of Senegal sole reared in the laboratory , 2001 .

[24]  M. Lynch,et al.  The evolutionary fate and consequences of duplicate genes. , 2000, Science.

[25]  H. Naka,et al.  Cloning and characterization of glyceraldehyde-3-phosphate dehydrogenase cDNA of Japanese flounder Paralichthys olivaceus† , 2000 .

[26]  M A Sirover,et al.  New insights into an old protein: the functional diversity of mammalian glyceraldehyde-3-phosphate dehydrogenase. , 1999, Biochimica et biophysica acta.

[27]  A. M. Schreiber,et al.  Metamorphosis in the summer flounder (Paralichthys dentatus): stage-specific developmental response to altered thyroid status. , 1998, General and comparative endocrinology.

[28]  T. Obinata,et al.  Effect of thyroid hormone on developmental transition of myosin light chains during flounder metamorphosis. , 1994, General and comparative endocrinology.

[29]  S. Miwa,et al.  Thyroid hormone stimulates gastric development in flounder larvae during metamorphosis , 1992 .

[30]  S. Miwa,et al.  Thyroid hormone stimulates the shift of erythrocyte populations during metamorphosis of the flounder , 1991 .

[31]  T. Hirano,et al.  Thyroxine surge in metamorphosing flounder larvae. , 1988, General and comparative endocrinology.

[32]  A. Benedetti,et al.  BMC Molecular Biology , 2009 .

[33]  J. Volff Genome evolution and biodiversity in teleost fish , 2005, Heredity.

[34]  K. Pittman,et al.  Thyroxine as a Mediator of Metamorphosis of Atlantic Halibut, Hippoglossus Hippoglossus , 2004, Environmental Biology of Fishes.

[35]  A. Meyer,et al.  Three Rounds (1r/2r/3r) of Genome Duplications and the Evolution of the Glycolytic Pathway in Vertebrates , 2022 .

[36]  Thomas D. Schmittgen,et al.  Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2 2 DD C T Method , 2022 .