METHOD DEVELOPMENT AND ESTIMATION OF ELETRIPTAN HYDROBROMIDE IN TABLET DOSAGE FORM BY RP-HPLC

Article history A simple, precise, rapid and accurate RP- HPLC method was developed for the estimation of Eletriptan Hydrobromide (ETP) in tablet dosage forms. An Inertsil ODS 3V, 250x4.6 mm, column with 5 μm particle size and the Mobile Phase consisting of 0.03M Ammonium Acetate in water adjusting the pH-3.2 with dilute O-Phosphoric Acid & TEA (0.5%) mixed in Methanol & Water in ratio of 40:60 v/v & Acetonitrile & Buffer solution (50:50 v/v) was used as a diluent in the gradient mode. The flow rate was 1.0 ml/min and the effluents were monitored at 222 nm. The retention time was 4.762 min and the detector response was linear in the concentration range of 50-600 µg/mL for ETP successively. The respective linear regression equation being Y= 10312.358x + 237007.9858 for ETP. The Limit of Detection (LOD) & The Limit of Quantification (LOQ) was found to be 2.5 & 7.5 µg/mL for ETP. The percentage assay of ETP was 99.93%. The method was validated by determining its accuracy, precision and system suitability. The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which is useful for the routine determination of ETP in bulk drug and in its pharmaceutical dosage forms.