Substrate affinity and catalytic efficiency are improved by decreasing glycosylation sites in Trichoderma reesei cellobiohydrolase I expressed in Pichia pastoris

[1]  Lushan Wang,et al.  Asn64-glycosylation affects Hypocrea jecorina (syn. Trichoderma reesei) cellobiohydrolase Cel7A activity expressed in Pichia pastoris , 2010 .

[2]  Michael E Himmel,et al.  Implications of cellobiohydrolase glycosylation for use in biomass conversion , 2008, Biotechnology for biofuels.

[3]  Xiang-mei Liu,et al.  Purification and characterization of recombinant endoglucanase of Trichoderma reesei expressed in Saccharomyces cerevisiae with higher glycosylation and stability. , 2008, Protein expression and purification.

[4]  P. Thibault,et al.  Identification of glycan structure and glycosylation sites in cellobiohydrolase II and endoglucanases I and II from Trichoderma reesei. , 2002, Glycobiology.

[5]  Emmanuel Guedon,et al.  Improvement of Cellulolytic Properties of Clostridium cellulolyticum by Metabolic Engineering , 2002, Applied and Environmental Microbiology.

[6]  T. Teeri,et al.  Characterization of Trichoderma reesei cellobiohydrolase Cel7A secreted from Pichia pastoris using two different promoters. , 2000, Biotechnology and bioengineering.

[7]  B. Imperiali,et al.  Effect of N-linked glycosylation on glycopeptide and glycoprotein structure. , 1999, Current opinion in chemical biology.

[8]  J. Ståhlberg,et al.  Cellobiohydrolase I from Trichoderma reesei: identification of an active-site nucleophile and additional information on sequence including the glycosylation pattern of the core protein. , 1997, Carbohydrate research.

[9]  C. Hollenberg,et al.  Production of recombinant proteins by methylotrophic yeasts. , 1997, Current opinion in biotechnology.

[10]  R. Contreras,et al.  Structural characterization of N-linked oligosaccharides from cellobiohydrolase I secreted by the filamentous fungus Trichoderma reesei RUTC 30. , 1997, European journal of biochemistry.

[11]  Stiefel Molding metallocenters for biology. , 1996, Chemistry & biology.

[12]  M. Weiner,et al.  Site-directed mutagenesis of double-stranded DNA by the polymerase chain reaction. , 1994, Gene.

[13]  T. K. Ghose Measurement of cellulase activities , 1987 .

[14]  A. Lappalainen,et al.  Cellobiohydrolase from Trichoderma reesei. , 1983, The Biochemical journal.

[15]  L. A. Palomares,et al.  Production of recombinant proteins: challenges and solutions. , 2004, Methods in molecular biology.

[16]  J. O. Baker,et al.  Cloning and Expression of Trichoderma reesei Cellobiohydrolase I in Pichia pastoris , 1999, Biotechnology progress.

[17]  N. Packer,et al.  Modified glycosylation of cellobiohydrolase I from a high cellulase-producing mutant strain of Trichoderma reesei. , 1998, European journal of biochemistry.

[18]  G. Gellissen,et al.  Gene expression in methylotrophic yeasts. , 1995, Bioprocess technology.