Substrate affinity and catalytic efficiency are improved by decreasing glycosylation sites in Trichoderma reesei cellobiohydrolase I expressed in Pichia pastoris
暂无分享,去创建一个
[1] Lushan Wang,et al. Asn64-glycosylation affects Hypocrea jecorina (syn. Trichoderma reesei) cellobiohydrolase Cel7A activity expressed in Pichia pastoris , 2010 .
[2] Michael E Himmel,et al. Implications of cellobiohydrolase glycosylation for use in biomass conversion , 2008, Biotechnology for biofuels.
[3] Xiang-mei Liu,et al. Purification and characterization of recombinant endoglucanase of Trichoderma reesei expressed in Saccharomyces cerevisiae with higher glycosylation and stability. , 2008, Protein expression and purification.
[4] P. Thibault,et al. Identification of glycan structure and glycosylation sites in cellobiohydrolase II and endoglucanases I and II from Trichoderma reesei. , 2002, Glycobiology.
[5] Emmanuel Guedon,et al. Improvement of Cellulolytic Properties of Clostridium cellulolyticum by Metabolic Engineering , 2002, Applied and Environmental Microbiology.
[6] T. Teeri,et al. Characterization of Trichoderma reesei cellobiohydrolase Cel7A secreted from Pichia pastoris using two different promoters. , 2000, Biotechnology and bioengineering.
[7] B. Imperiali,et al. Effect of N-linked glycosylation on glycopeptide and glycoprotein structure. , 1999, Current opinion in chemical biology.
[8] J. Ståhlberg,et al. Cellobiohydrolase I from Trichoderma reesei: identification of an active-site nucleophile and additional information on sequence including the glycosylation pattern of the core protein. , 1997, Carbohydrate research.
[9] C. Hollenberg,et al. Production of recombinant proteins by methylotrophic yeasts. , 1997, Current opinion in biotechnology.
[10] R. Contreras,et al. Structural characterization of N-linked oligosaccharides from cellobiohydrolase I secreted by the filamentous fungus Trichoderma reesei RUTC 30. , 1997, European journal of biochemistry.
[11] Stiefel. Molding metallocenters for biology. , 1996, Chemistry & biology.
[12] M. Weiner,et al. Site-directed mutagenesis of double-stranded DNA by the polymerase chain reaction. , 1994, Gene.
[13] T. K. Ghose. Measurement of cellulase activities , 1987 .
[14] A. Lappalainen,et al. Cellobiohydrolase from Trichoderma reesei. , 1983, The Biochemical journal.
[15] L. A. Palomares,et al. Production of recombinant proteins: challenges and solutions. , 2004, Methods in molecular biology.
[16] J. O. Baker,et al. Cloning and Expression of Trichoderma reesei Cellobiohydrolase I in Pichia pastoris , 1999, Biotechnology progress.
[17] N. Packer,et al. Modified glycosylation of cellobiohydrolase I from a high cellulase-producing mutant strain of Trichoderma reesei. , 1998, European journal of biochemistry.
[18] G. Gellissen,et al. Gene expression in methylotrophic yeasts. , 1995, Bioprocess technology.