Effects of photo stimulation and nonstimulation of golden hamsters (Mesocricetus auratus) from birth to early puberty on testes structure and function.

We tested whether puberty in golden hamsters is photoperiodically controlled. Hamsters were raised under 14:10 hours Light:Dark (14L) and 1:23 hours Light:Dark (1L) respectively, from birth to 28 days and tested for various parameters. Body weight, Leydig cell (LC) size and testicular testosterone secretion were greater and plasma thyroxin (T4), testicular androstenedione secretion and LC number were lower (P<0.05) in 1L than 14L hamsters. Volumes of testicular components were similar in the two groups. 3beta-hydroxy steroid dehydrogenase immunohistochemistry demonstrated LC progenitors and newly formed adult LC (ALC) in 14L hamsters, which were absent in 1L hamsters; they contained only fetal LC (FLC). Latter findings suggest the presence and absence of postnatally-differerentiated LC in 14L and 1L hamsters, respectively. Androgen results agreed with these findings, because FLC primarily secrete testosterone, and androstenedione is a major androgen secreted by the newly formed ALC. Reduced T4 in 1L hamsters is attributed to the inhibition of thyroid function by the increased duration of melatonin secretion due to non-photostimulatory conditions. The arrest in LC differentiation in 1L hamsters is attributed to low T4 levels. Although the testis size is unaltered under non-photostimulatory conditions, postnatal LC differentiation is inhibited in golden hamsters, and therefore, it is logical to suggest that their puberty is photoperiodically controlled.

[1]  I. Zucker,et al.  Maternal Photoperiodic History Affects Offspring Development in Syrian Hamsters , 2008, Journal of biological rhythms.

[2]  G. Iwamoto,et al.  Impact of neonatal hypothyroidism on reproduction in the male hamster, Mesocricetus auratus , 2007, Physiology & Behavior.

[3]  P. Pévet,et al.  Melatonin regulates type 2 deiodinase gene expression in the Syrian hamster. , 2006, Endocrinology.

[4]  P. Baker,et al.  Neuroendocrine Regulation of Leydig Cell Development , 2005, Annals of the New York Academy of Sciences.

[5]  O. Pignataro,et al.  Direct effect of melatonin on Syrian hamster testes: melatonin subtype 1a receptors, inhibition of androgen production, and interaction with the local corticotropin-releasing hormone system. , 2005, Endocrinology.

[6]  W. Jin,et al.  Effects of hypothyroidism on gonadal function after transition of short day photoperiod in male golden hamsters (Mesocricetus auratus). , 2005, The Journal of reproduction and development.

[7]  C. Mendis-Handagama,et al.  PROLONGED AND TRANSIENT NEONATAL HYPOTHYROIDISM ON LEYDIG CELL DIFFERENTIATION IN THE POSTNATAL RAT TESTIS , 2004, Archives of andrology.

[8]  M. Rettori,et al.  S22153, a melatonin antagonist, dissociates different aspects of photoperiodic responses in Syrian hamsters , 2003, Behavioural Brain Research.

[9]  S. C. Mendis-Handagama,et al.  Differentiation of the adult Leydig cell population in the postnatal testis. , 2001, Biology of reproduction.

[10]  N. Mills,et al.  Effects of Thyroid Hormone on Leydig Cell Regeneration in the Adult Rat Following Ethane Dimethane Sulphonate Treatment1 , 2000, Biology of reproduction.

[11]  J. Mason,et al.  Effects of Thyroid and Luteinizing Hormones on the Onset of Precursor Cell Differentiation into Leydig Progenitor Cells in the Prepubertal Rat Testis1 , 2000, Biology of reproduction.

[12]  D. Hales,et al.  Studies on the Onset of Leydig Precursor Cell Differentiation in the Prepubertal Rat Testis , 2000 .

[13]  H. Ariyaratne,et al.  Changes in the Testis Interstitium of Sprague Dawley Rats from Birth to Sexual Maturity1 , 2000, Biology of reproduction.

[14]  H. Ariyaratne,et al.  Differentiation of adult Leydig cells in the neonatal rat testis is arrested by hypothyroidism. , 1998, Biology of reproduction.

[15]  P. Saunders,et al.  Immunoexpression of the steroidogenic enzymes 3-beta hydroxysteroid dehydrogenase and 17 alpha-hydroxylase, C17,20 lyase and the receptor for luteinizing hormone (LH) in the fetal rat testis suggests that the onset of Leydig cell steroid production is independent of LH action. , 1998, Biology of reproduction.

[16]  R. Donham,et al.  Dissociation of the reproductive and prolactin photoperiodic responses in male golden hamsters. , 1994, Biology of reproduction.

[17]  I. Huhtaniemi,et al.  Fetal Leydig Cells: Cellular Origin, Morphology, Life Span, and Special Functional Features , 1992, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine.

[18]  J. Kerr,et al.  Experimental cryptorchidism in the adult mouse: II. A hormonal study. , 1990, Journal of andrology.

[19]  S M Mendis-Handagama,et al.  Sources of error in the estimation of Leydig cell numbers in control and atrophied mammalian testes , 1990, Journal of microscopy.

[20]  J. Mason,et al.  Human 3 beta-hydroxysteroid dehydrogenase/delta 5----4isomerase from placenta: expression in nonsteroidogenic cells of a protein that catalyzes the dehydrogenation/isomerization of C21 and C19 steroids. , 1990, Endocrinology.

[21]  F. Turek,et al.  Reproductive responsiveness to short photoperiod develops postnatally in male golden hamsters. , 1987, Journal of andrology.

[22]  M. Stetson,et al.  Functional maturation of the gonads of Turkish hamsters under various photoperiods. , 1986, Biology of reproduction.

[23]  T. Bartness,et al.  Photoperiodic control of seasonal body weight cycles in hamsters , 1985, Neuroscience & Biobehavioral Reviews.

[24]  J. Vriend.  Effects of melatonin and thyroxine replacement on thyrotropin, luteinizing hormone, and prolactin in male hypothyroid hamsters. , 1985, Endocrinology.

[25]  J. Vriend,et al.  Effects of pinealectomy and melatonin administration on thyroid follicles of blind syrian hamsters , 1985, The Anatomical record.

[26]  D. C. Sterio.  The unbiased estimation of number and sizes of arbitrary particles using the disector , 1984, Journal of microscopy.

[27]  B. Goldman,et al.  Photoperiod control of reproductive development in the male Djungarian hamster (Phodopus sungorus). , 1984, Endocrinology.

[28]  T. Bartness,et al.  Photoperiodic control of body weight and energy metabolism in Syrian hamsters (Mesocricetus auratus): role of pineal gland, melatonin, gonads, and diet. , 1984, Endocrinology.

[29]  F. Turek,et al.  Gonadal growth and gonadal hormones do not participate in the development of responsiveness to photoperiod in the golden hamster. , 1983, Biology of reproduction.

[30]  M. Rollag,et al.  Ontogeny of the gonadal response of golden hamsters to short photoperiod, blinding, and melatonin. , 1982, Biology of reproduction.

[31]  F. Davis,et al.  Influence of photoperiod on reproductive development in the golden hamster. , 1980, Biology of reproduction.

[32]  Elliott Ja.  Circadian rhythms and photoperiodic time measurement in mammals. , 1976 .

[33]  R. A. Hoffman,et al.  Early photoperiodic conditions and pineal antigonadal function in male hamsters. , 1970, International journal of fertility.

[34]  M. Menaker,et al.  Photoperiodic Control of Hamster Testis , 1967, Science.

[35]  B. Zirkin,et al.  Comparison of components of the testis interstitium with testosterone secretion in hamster, rat, and guinea pig testes perfused in vitro. , 1988, The American journal of anatomy.

[36]  L. Russel,et al.  Ultrastructure of leydig cells as revealed by secondary tissue treatment with a ferrocyanide-osmium mixture. , 1977, Tissue & cell.

[37]  E. Weibel.  Stereological principles for morphometry in electron microscopic cytology. , 1969, International review of cytology.