Engineered Branching Peptide as Dual-Functional Antifouling and Recognition Probe: Toward a Dual-Photoelectrode Protein Biosensor with High Accuracy.

The building of practical biosensors that have anti-interference abilities against biofouling of nonspecific proteins and biooxidation of reducing agents in actual biological matrixes remains a great challenge. Herein, a robust photoelectrochemical (PEC) biosensor capable of accurate detection in human serum was pioneered through the integration of a new engineered branching peptide (EBP) into a synergetic dual-photoelectrode system. The synergetic dual-photoelectrode system involved the tandem connection of a C3N4/TiO2 photoanode and a AuPt/PANI photocathode, while the EBP as a dual-functional antifouling and recognition probe featured an inverted Y-shaped configuration with one recognition backbone and two antifouling branches. Such an EBP enables a simple procedure for electrode modification and an enhanced antifouling nature compared to a regular linear peptide (LP), as theoretically supported by the results from molecular dynamics simulations. The as-developed PEC biosensor had a higher photocurrent response and a good antioxidation property inherited from the photoanode and photocathode, respectively. Targeting the model protein biomarker of cardiac troponin I (cTnI), this biosensor achieved good performances in terms of high sensitivity, specificity, and anti-interference.

[1]  C. Li,et al.  An electrochemical biosensor based on electroactive peptide nanoprobes for the sensitive analysis of tumor cells. , 2022, Biosensors & bioelectronics.

[2]  S. Luo,et al.  Intrinsically Disordered Protein Condensate-Modified Surface for Mitigation of Biofouling and Foreign Body Response. , 2022, Journal of the American Chemical Society.

[3]  Hongyuan Chen,et al.  Bipolar Modulation of the Ionic Circuit for Generic Organic Photoelectrochemical Transistor Logic and Sensor , 2022, Advanced Optical Materials.

[4]  Xiang Ren,et al.  Peptide-Based Biosensor with a Luminescent Copper-Based Metal-Organic Framework as an Electrochemiluminescence Emitter for Trypsin Assay. , 2021, Analytical chemistry.

[5]  A. Rosenhahn,et al.  Low fouling peptides with an all (D) amino acid sequence provide enhanced stability against proteolytic degradation while maintaining low antifouling properties. , 2020, Langmuir : the ACS journal of surfaces and colloids.

[6]  Ping Yu,et al.  Natural Leukocyte Membrane-Masked Microelectrodes with Enhanced Antifouling Ability and Biocompatibility for In Vivo Electrochemical Sensing. , 2020, Analytical chemistry.

[7]  Yoshihiro Ito,et al.  Strategy to Immobilize Peptide Probe Selected through in Vitro Ribosome Display for Electrochemical Aptasensor Application. , 2020, Analytical chemistry.

[8]  Robert Hein,et al.  Antifouling Strategies for Selective In Vitro and In Vivo Sensing. , 2020, Chemical reviews.

[9]  Jinghua Yu,et al.  Cathode Photoelectrochemical Paper Device for microRNA Detection Based on Cascaded Photoactive Structures and Hemin/Pt Nanoparticles Decorated DNA Dendrimers. , 2020, ACS applied materials & interfaces.

[10]  Hao Wu,et al.  Peptide-based Biosensing of Redox-active Protein-Heme Complexes Indicates Novel Mechanism for Tumor Survival under Oxidative Stress. , 2019, ACS sensors.

[11]  Jason J. Davis,et al.  Electrochemical Aptasensor for Ultralow Fouling Cancer Cell Quantification in Complex Biological Media Based on Designed Branched Peptides. , 2019, Analytical chemistry.

[12]  Deman Han,et al.  Cathodic photoelectrochemical bioanalysis , 2019, TrAC Trends in Analytical Chemistry.

[13]  Gaochao Fan,et al.  Nanoporous Semiconductor Electrode Captures the Quantum Dots: Toward Ultrasensitive Signal-On Liposomal Photoelectrochemical Immunoassay. , 2019, Analytical chemistry.

[14]  Xiliang Luo,et al.  Universal Design of Selectivity-Enhanced Photoelectrochemical Enzyme Sensor: Integrating Photoanode with Biocathode. , 2018, Analytical chemistry.

[15]  Xiliang Luo,et al.  Antifouling aptasensor for the detection of adenosine triphosphate in biological media based on mixed self-assembled aptamer and zwitterionic peptide. , 2018, Biosensors & bioelectronics.

[16]  Guobin Qi,et al.  Self‐Assembled Peptide‐Based Nanomaterials for Biomedical Imaging and Therapy , 2018, Advanced materials.

[17]  Zhen Liu,et al.  Peptide templated AuPt alloyed nanoparticles as highly efficient Bi-functional electrocatalysts for both oxygen reduction reaction and hydrogen evolution reaction , 2018 .

[18]  Dianping Tang,et al.  Novel photoelectrochemical immunosensor for disease-related protein assisted by hemin/G-quadruplex-based DNAzyme on gold nanoparticles to enhance cathodic photocurrent on p-CuBi2O4 semiconductor. , 2017, Biosensors & bioelectronics.

[19]  Wei-Wei Zhao,et al.  Hybrid PbS Quantum Dot/Nanoporous NiO Film Nanostructure: Preparation, Characterization, and Application for a Self-Powered Cathodic Photoelectrochemical Biosensor. , 2017, Analytical chemistry.

[20]  R. Niessner,et al.  Signal-On Photoelectrochemical Immunoassay for Aflatoxin B1 Based on Enzymatic Product-Etching MnO2 Nanosheets for Dissociation of Carbon Dots. , 2017, Analytical chemistry.

[21]  Carmay Lim,et al.  How Molecular Size Impacts RMSD Applications in Molecular Dynamics Simulations. , 2017, Journal of chemical theory and computation.

[22]  Sunghyun Kim,et al.  Persistent Hydrogen Production by the Photo-Assisted Microbial Electrolysis Cell Using a p-Type Polyaniline Nanofiber Cathode. , 2016, ChemSusChem.

[23]  B. Liedberg,et al.  Peptide Functionalized Gold Nanoparticles with Optimized Particle Size and Concentration for Colorimetric Assay Development: Detection of Cardiac Troponin I , 2016 .

[24]  Zhenzhen Li,et al.  Phosphorus Cation Doping: A New Strategy for Boosting Photoelectrochemical Performance on TiO2 Nanotube Photonic Crystals. , 2016, ACS applied materials & interfaces.

[25]  Jun‐Jie Zhu,et al.  Cathode Photoelectrochemical Immunosensing Platform Integrating Photocathode with Photoanode. , 2016, Analytical chemistry.

[26]  Guohua Chen,et al.  Self-assembly graphitic carbon nitride quantum dots anchored on TiO2 nanotube arrays: An efficient heterojunction for pollutants degradation under solar light. , 2016, Journal of hazardous materials.

[27]  Yuehe Lin,et al.  Enhanced Photoelectrochemical Immunosensing Platform Based on CdSeTe@CdS:Mn Core-Shell Quantum Dots-Sensitized TiO2 Amplified by CuS Nanocrystals Conjugated Signal Antibodies. , 2016, Analytical chemistry.

[28]  Shangfeng Yang,et al.  Incorporating Graphitic Carbon Nitride (g‐C3N4) Quantum Dots into Bulk‐Heterojunction Polymer Solar Cells Leads to Efficiency Enhancement , 2016 .

[29]  Jeong-Woo Choi,et al.  Three-dimensional crumpled graphene-based platinum-gold alloy nanoparticle composites as superior electrocatalysts for direct methanol fuel cells , 2015 .

[30]  Renliang Huang,et al.  Superior Antifouling Performance of a Zwitterionic Peptide Compared to an Amphiphilic, Non-Ionic Peptide. , 2015, ACS applied materials & interfaces.

[31]  Miaoyu Li,et al.  An all-solid-state perovskite-sensitized solar cell based on the dual function polyaniline as the sensitizer and p-type hole-transporting material , 2014 .

[32]  Q. Gao,et al.  Ultrasensitive electrogenerated chemiluminescence peptide-based method for the determination of cardiac troponin I incorporating amplification of signal reagent-encapsulated liposomes. , 2013, Analytical chemistry.

[33]  Peng Wang,et al.  Plasmonic gold nanocrystals coupled with photonic crystal seamlessly on TiO2 nanotube photoelectrodes for efficient visible light photoelectrochemical water splitting. , 2013, Nano letters.

[34]  M. Marchi,et al.  Molecular Dynamics Simulations of a Characteristic DPC Micelle in Water. , 2012, Journal of chemical theory and computation.

[35]  Andrew D White,et al.  Sequence, structure, and function of peptide self-assembled monolayers. , 2012, Journal of the American Chemical Society.

[36]  Hexing Li,et al.  Nanotube-confinement induced size-controllable g-C3N4 quantum dots modified single-crystalline TiO2 nanotube arrays for stable synergetic photoelectrocatalysis , 2016 .

[37]  M. Antonietti,et al.  A metal-free polymeric photocatalyst for hydrogen production from water under visible light. , 2009, Nature materials.