Detection of Strongyloides stercoralis in Tierralta, Colombia using four parasitological methods

Introduction: soil-borne helminth Strongyloides stercoralis is one of the most neglected among neglected tropical diseases. A study was conducted of the presence of S. stercoralis in a village from the department of Cordoba, Colombia, with the purpose of comparing the effectiveness of several diagnostic methods. Methods: stool samples from 262 persons were evaluated. Each sample was examined with four parasitological techniques: direct examination, agar plate culture (APC), the modified Baermann method, and the Harada-Mori technique. Results: S. stercoralis was detected by at least one of the techniques in four of the 262 samples: the Harada-Mori technique detected 2 cases, APC 1 case and direct examination 1 case. The modified Baermann method did not detect any case. No significant differences were found when comparing the techniques. Conclusions: results show that S. stercoralis is not endemic in the village of Cordoba, and that parasitological techniques should be used in combination to improve the quality of diagnosis.

[1]  P. Vounatsou,et al.  Strongyloides stercoralis: Global Distribution and Risk Factors , 2013, PLoS neglected tropical diseases.

[2]  Frine Samalvides Cuba,et al.  Evaluación de técnicas parasitológicas en el diagnóstico de estrongiloidiasis por Strongyloides stercoralis , 2012 .

[3]  P. Steinmann,et al.  Strongyloidiasis--the most neglected of the neglected tropical diseases? , 2009, Transactions of the Royal Society of Tropical Medicine and Hygiene.

[4]  H. Dupont,et al.  Strongyloides hyperinfection syndrome: an emerging global infectious disease. , 2008, Transactions of the Royal Society of Tropical Medicine and Hygiene.

[5]  J. Utzinger,et al.  Occurrence of Strongyloides stercoralis in Yunnan Province, China, and Comparison of Diagnostic Methods , 2007, PLoS neglected tropical diseases.

[6]  Peter J Hotez,et al.  Soil-transmitted helminth infections: ascariasis, trichuriasis, and hookworm , 2006, The Lancet.

[7]  P. Bloch,et al.  Distribution of human Oesophagostomum bifurcum, hookworm and Strongyloides stercoralis infections in northern Ghana. , 2005, Transactions of the Royal Society of Tropical Medicine and Hygiene.

[8]  Leonardo Elías Ordóñez Ordóñez,et al.  Desnutrición y su relación con parasitismo intestinal en niños de una población de la Amazonia colombiana. , 2002 .

[9]  L. E. Ordóñez,et al.  [Malnutrition and its association with intestinal parasitism among children from a village in the Colombian Amazonian region]. , 2002, Biomedica : revista del Instituto Nacional de Salud.

[10]  S. Berk,et al.  Diagnosis of Strongyloides stercoralis infection. , 2001, Clinical infectious diseases : an official publication of the Infectious Diseases Society of America.

[11]  F. Hernández-Chavarría,et al.  A simple modification of the Baermann method for diagnosis of strongyloidiasis. , 2001, Memorias do Instituto Oswaldo Cruz.

[12]  C. Putaporntip,et al.  Increased sensitivity of routine laboratory detection of Strongyloides stercoralis and hookworm by agar-plate culture. , 1999, Transactions of the Royal Society of Tropical Medicine and Hygiene.

[13]  P. Uparanukraw,et al.  Fluctuations of larval excretion in Strongyloides stercoralis infection. , 1999, The American journal of tropical medicine and hygiene.

[14]  A. Foli,et al.  Studies on prevalence of Strongyloides infection in Holambra and Maceió, Brazil, by the agar plate faecal culture method. , 1996, Revista do Instituto de Medicina Tropical de Sao Paulo.

[15]  J. Kobayashi,et al.  Efficacy of stool examination for detection of Strongyloides infection. , 1995, The American journal of tropical medicine and hygiene.

[16]  D. Conway,et al.  Strongyloides stercoralis in an urban slum community in Bangladesh: factors independently associated with infection. , 1994, Transactions of the Royal Society of Tropical Medicine and Hygiene.

[17]  N. K. Mahdi,et al.  Diagnostic methods for intestinal parasites in southern Iraq with reference to Strongyloides stercoralis. , 1993, The Southeast Asian journal of tropical medicine and public health.

[18]  D. Rg.  Evaluation of three methods for laboratory diagnosis of Strongyloides stercoralis infection. , 1993 .

[19]  R. G. de Kaminsky.  Evaluation of three methods for laboratory diagnosis of Strongyloides stercoralis infection. , 1993, The Journal of parasitology.

[20]  S. Kasuya,et al.  How effective is the agar plate method for Strongyloides stercoralis? , 1992, The Journal of parasitology.

[21]  K Koga,et al.  A modified agar plate method for detection of Strongyloides stercoralis. , 1991, The American journal of tropical medicine and hygiene.

[22]  M. Iwanaga,et al.  Efficacy of agar-plate culture in detection of Strongyloides stercoralis infection. , 1990, The Journal of parasitology.

[23]  F. W. Nugent,et al.  Strongyloides stercoralis. , 1961, The Lahey Clinic bulletin.

[24]  Y. Harada,et al.  A New Method for culturing Hook Worm. , 1955 .

[25]  L. S. Ritchie.  An ether sedimentation technique for routine stool examinations. , 1948, Bulletin of the U.S. Army Medical Department. United States. Army. Medical Department.

[26]  Ritchie Ls.  ETHER sedimentation technique for routine stool examinations. , 1948, Circular letter. United States. Far East Command. Medical Section.