Highly sensitive electrochemical detection of dopamine and uric acid on a novel carbon nanotube-modified ionic liquid-nanozeolite paste electrode

A novel biosensor has been constructed by incorporating modified nanosized natural zeolite and 3-hydroxypropanaminium acetate (HPAA) as a novel room temperature ionic liquid, supported on multiwalled carbon nanotube (MWCNTs) and employed for the simultaneous determination of dopamine (DA) and uric acid (UA). A detailed investigation by transmission electron microscopy and electrochemistry is performed in order to elucidate the preparation process and properties of the composites. The voltammetric studies using the modified carbon paste electrode show two well-resolved anodic peaks for DA and UA with a potential difference of 160 mV, revealing the possibility of the simultaneous electrochemical detection of these compounds. The modified carbon paste electrode shows good conductivity, stability, and extraction effect due to the synergic action of HPAA, MWCNTs, and iron ion-doped natrolite zeolite. Under optimized conditions, the peak currents are linear from 8.12 × 10−7 to 3.01 × 10−4 mol L−1 and from 9.31 × 10−7 to 3.36 × 10−4 mol L−1 with detection limits of 1.16 × 10−7 and 1.33 × 10−7 mol L−1 for DA and UA using the differential pulse voltammetric method, respectively. Finally, the modified carbon paste electrode proved to have good sensitivity and stability and is successfully applied for the simultaneous determination of DA and UA in human blood serum and urine samples.

[1]  J. Hart,et al.  Voltammetric behaviour of ascorbic acid at a graphite-epoxy composite electrode chemically modified with cobalt phthalocyanine and its amperometric determination in multivitamin preparations , 1990 .

[2]  Yaping Ding,et al.  Simultaneous determination of dopamine and uric acid on nafion/sodium dodecylbenzenesulfonate composite film modified glassy carbon electrode , 2009 .

[3]  M. Noroozifar,et al.  Adsorption behavior of Cr(VI) on modified natural zeolite by a new bolaform N,N,N,N',N',N'-hexamethyl-1,9-nonanediammonium dibromide reagent. , 2008, Journal of hazardous materials.

[4]  B. Rezaei,et al.  Voltammetric determination of dopamine in the presence of uric acid using a 2-hydroxy-1-(1-hydroxynaphthyl-2-azo)- -naphthalin-4-sulfonic acid modified glassy carbon electrode , 2010 .

[5]  P. Pickup,et al.  Electronically conductive anion exchange polymers based on polypyrrole: Preparation, characterization, electrostatic binding of ferrocyanide and electrocatalysis of ascorbic acid oxidation , 1989 .

[6]  T. Imato,et al.  Potentiometric butyrylcholine sensor for organophosphate pesticides. , 1995, Biosensors & bioelectronics.

[7]  E. Wang,et al.  Studies of interaction between iron (III) and intermediates of synthetic neuromelanin by means of cyclic voltammetry of Fe (CN)3- 6 and dopamine , 2006 .

[8]  Wei Sun,et al.  Electrochemistry and electrocatalysis of hemoglobin in Nafion/nano-CaCO3 film on a new ionic liquid BPPF6 modified carbon paste electrode. , 2007, The journal of physical chemistry. B.

[9]  Haoshen Zhou,et al.  Amperometric biosensor based on tyrosinase-conjugated polysaccharide hybrid film: selective determination of nanomolar neurotransmitters metabolite of 3,4-dihydroxyphenylacetic acid (DOPAC) in biological fluid. , 2005, Biosensors & bioelectronics.

[10]  R. Compton,et al.  Room Temperature Ionic Liquid Carbon Nanotube Paste Electrodes: Overcoming Large Capacitive Currents Using Rotating Disk Electrodes , 2007 .

[11]  Ping Wu,et al.  Immobilization and direct electrochemistry of cytochrome c at a single-walled carbon nanotube-modified electrode , 2006 .

[12]  P. Ajayan.  Nanotubes from Carbon. , 1999, Chemical reviews.

[13]  P. Bouř,et al.  Infrared absorption detection of metal ion-deoxyguanosine monophosphate binding: experimental and theoretical study. , 2009, The journal of physical chemistry. B.

[14]  Wanzhi. Wei,et al.  Assemble-Electrodeposited Ultrathin Conducting Poly(Azure A) at a Carbon Nanotube-Modified Glassy Carbon Electrode, and its Electrocatalytic Properties to the Reduction of Nitrite , 2006 .

[15]  B. Ogorevc,et al.  Simultaneous measurement of dopamine and ascorbate at their physiological levels using voltammetric microprobe based on overoxidized poly(1,2-phenylenediamine)-coated carbon fiber. , 2001, Analytical chemistry.

[16]  F. Tajabadi,et al.  Simultaneous determination of dopamine, ascorbic acid, and uric acid using carbon ionic liquid electrode. , 2006, Analytical biochemistry.

[17]  Ping Wu,et al.  Preparation and characterization of room temperature ionic liquid/single-walled carbon nanotube nanocomposites and their application to the direct electrochemistry of heme-containing proteins/enzymes , 2007 .

[18]  R. Adams,et al.  Probing brain chemistry with electroanalytical techniques. , 1976, Analytical chemistry.

[19]  L. Qian,et al.  Composite film of carbon nanotubes and chitosan for preparation of amperometric hydrogen peroxide biosensor. , 2006, Talanta.

[20]  Alain Walcarius,et al.  Zeolite-modified electrodes in electroanalytical chemistry , 1999 .

[21]  R. E. Adams,et al.  Electrochemical pH-stat and controlled current coulometric acid-base analyzer , 1976 .

[22]  J. B. Justice,et al.  Peer Reviewed: Probing Brain Chemistry: Voltammetry Comes of Age , 1996 .

[23]  Wen-Jun Guan,et al.  Glucose biosensor based on multi-wall carbon nanotubes and screen printed carbon electrodes. , 2005, Biosensors & bioelectronics.

[24]  B. García,et al.  Alkali-metal ion catalysis of the oxidation of L-ascorbic acid by hexacyanoferrate(III) in strongly acidic media , 1993 .

[25]  Guanghan Lu,et al.  Simultaneous Determination of Dopamine and Uric Acid at 2-Amino-5-mercapto-[1,3,4] Triazole Self-assembled Monolayers Gold Electrode , 2005 .

[26]  A. Arabzadeh,et al.  Simultaneous Determination of Dopamine and Uric Acid by Electrocatalytic Oxidation on a Carbon Paste Electrode Using Pyrogallol Red as a Mediator , 2010 .

[27]  P. Kissinger,et al.  Analytical methods for measuring uric acid in biological samples and food products. , 1987, Journal - Association of Official Analytical Chemists.

[28]  H. Ngo,et al.  Thermal properties of imidazolium ionic liquids , 2000 .

[29]  Fwu-Shan Sheu,et al.  Application of multi-walled carbon nanotubes functionalized with hemin for oxygen detection in neutral solution , 2004 .

[30]  S. Omaye,et al.  An internal standard method for the unattended high-performance liquid chromatographic analysis of ascorbic acid in blood components. , 1987, Analytical biochemistry.

[31]  Federica Valentini,et al.  Carbon nanotubes as electrode materials for the assembling of new electrochemical biosensors , 2004 .

[32]  R. Gropeanu,et al.  Carbon paste electrodes modified with a new phenothiazine derivative adsorbed on zeolite and on mineral clay for NADH oxidation , 2009 .

[33]  Shen-Ming Chen,et al.  Poly(3,4-ethylenedioxythiophene-co-(5-amino-2-naphthalenesulfonic acid)) (PEDOT-PANS) film modified glassy carbon electrode for selective detection of dopamine in the presence of ascorbic acid and uric acid. , 2007, Analytica chimica acta.

[34]  S. Dong,et al.  Electrochemical Characteristics of Glucose Oxidase Adsorbed at Carbon Nanotubes Modified Electrode with Ionic Liquid as Binder , 2007 .

[35]  Yongxin Li,et al.  Fabrication of layer-by-layer modified multilayer films containing choline and gold nanoparticles and its sensing application for electrochemical determination of dopamine and uric acid. , 2007, Talanta.

[36]  George G. Guilbault,et al.  Analytical Uses of Immobilized Enzymes , 1984 .

[37]  J. Cassidy,et al.  Ascorbic acid oxidation at polypyrrole-coated electrodes , 1991 .

[38]  S. Sampath,et al.  Selective Determination of Uric Acid in Presence of Ascorbic Acid and Dopamine at Neutral pH Using Exfoliated Graphite Electrodes , 2004 .

[39]  Liping Jiang,et al.  Study on the Electrochemical Behavior of Dopamine and Uric Acid at a 2‐Amino‐5‐mercapto‐[1,3,4] Triazole Self‐Assembled Monolayers Electrode , 2006 .

[40]  W. Horsthemke,et al.  Comparison of zeolite-modified electrodes with a graphite electrode : postulated enhancement of electroactivity through stabilization of intermediates , 1993 .

[41]  H. Shaterian,et al.  A simple Green approach to the synthesis of 2-amino-5-oxo-4,5-dihydropyrano[3,2-c]chromene-3-carbonitrile derivatives catalyzed by 3-hydroxypropanaminium acetate (HPAA) as a new ionic liquid , 2011 .

[42]  M. Moawad.  Complexation and Thermal Studies of Uric Acid with Some Divalent and Trivalent Metal Ions of Biological Interest in the Solid State , 2002 .

[43]  M. Noroozifar,et al.  Cyanide uptake from wastewater by modified natrolite zeolite-iron oxyhydroxide system: application of isotherm and kinetic models. , 2009, Journal of hazardous materials.

[44]  Jing Chen,et al.  Direct electron transfer of glucose oxidase promoted by carbon nanotubes. , 2004, Analytical biochemistry.