Osteoblastic differentiating potential of dental pulp stem cells in vitro cultured on a chemically modified microrough titanium surface.

Titanium surface modification is critical for dental implant success. Our aim was to determine surfaces influence on dental pulp stem cells (DPSCs) viability and differentiation. Implants were divided into sandblasted/acid-etched (control) and sandblasted/acid-etched coated with calcium and magnesium ions (CaMg), supplied as composite (test). Proliferation was evaluated by MTT, differentiation checking osteoblastic gene expression, PGE2 secretion and matrix formation, inflammation by Interleukin 6 (IL-6) detection. MTT and IL-6 do not modify on test. A PGE2 increase on test is recorded. BMP2 is higher on test at early experimental points, Osterix and RUNX2 augment later. Alizarin-red S reveals higher matrix production on test. These results suggest that test surface is more osteoinductive, representing a start point for in vivo studies aiming at the construction of more biocompatible dental implants, whose integration and clinical performance are improved and some undesired effects, such as implant stability loss and further surgical procedures, are reduced.

[1]  J. E. Maté Sánchez de Val,et al.  Effects on the osseointegration of titanium implants incorporating calcium–magnesium: a resonance frequency and histomorphometric analysis in rabbit tibia , 2018, Clinical oral implants research.

[2]  T. Kawamoto,et al.  Role of MSX1 in Osteogenic Differentiation of Human Dental Pulp Stem Cells , 2016, Stem cells international.

[3]  A. Cataldi,et al.  In Vitro Behavior of Primary Human Osteoblasts Onto Microrough Titanium Surface , 2015, Implant dentistry.

[4]  N. Dubey,et al.  Bioactivity, physical and chemical properties of MTA mixed with propylene glycol , 2015, Journal of applied oral science : revista FOB.

[5]  B. Arumugam,et al.  Runx2: Structure, function, and phosphorylation in osteoblast differentiation. , 2015, International journal of biological macromolecules.

[6]  Y. Mortazavi,et al.  Different Methylation Patterns of RUNX2, OSX, DLX5 and BSP in Osteoblastic Differentiation of Mesenchymal Stem Cells , 2015, Cell journal.

[7]  I. D'alimonte,et al.  Molecular and phenotypic characterization of human amniotic fluid-derived cells: a morphological and proteomic approach. , 2015, Stem cells and development.

[8]  M. Frech,et al.  Niemann-Pick type C1 patient-specific induced pluripotent stem cells display disease specific hallmarks , 2013, Orphanet Journal of Rare Diseases.

[9]  J. Shibli,et al.  Influence of titanium surface treatments on formation of the blood clot extension. , 2011, The Journal of oral implantology.

[10]  H. Du,et al.  Osteoblastic cell response on fluoridated hydroxyapatite coatings: the effect of magnesium incorporation , 2010, Biomedical materials.

[11]  T. Albrektsson,et al.  Effects of titanium surface topography on bone integration: a systematic review. , 2009, Clinical oral implants research.

[12]  S. Ferguson,et al.  Potential of chemically modified hydrophilic surface characteristics to support tissue integration of titanium dental implants. , 2009, Journal of biomedical materials research. Part B, Applied biomaterials.

[13]  Gianpaolo Papaccio,et al.  Dental Pulp Stem Cells: A Promising Tool for Bone Regeneration , 2008, Stem Cell Reviews.

[14]  O. Matsuo,et al.  Mesenchymal progenitor cells in adult human dental pulp and their ability to form bone when transplanted into immunocompromised mice , 2007, Cell biology international.

[15]  M. Wieland,et al.  The initial attachment and subsequent behavior regulation of osteoblasts by dental implant surface modification. , 2007, Journal of biomedical materials research. Part A.

[16]  Anthony Atala,et al.  Isolation of amniotic stem cell lines with potential for therapy , 2007, Nature Biotechnology.

[17]  M. Sampaolesi,et al.  Human postnatal dental pulp cells co-differentiate into osteoblasts and endotheliocytes: a pivotal synergy leading to adult bone tissue formation , 2007, Cell Death and Differentiation.

[18]  S Tetè,et al.  In Vitro Stem Cell Cultures from Human Dental Pulp and Periodontal Ligament: New Prospects in Dentistry , 2007, International journal of immunopathology and pharmacology.

[19]  S. Gronthos,et al.  Stem cells in the periodontal ligament. , 2006, Oral diseases.

[20]  J. Jansen,et al.  The effects of implant surface roughness and surgical technique on implant fixation in an in vitro model. , 2006, Clinical oral implants research.

[21]  Andrea Bagno,et al.  Surface treatments and roughness properties of Ti-based biomaterials , 2004, Journal of materials science. Materials in medicine.

[22]  H. Nygren,et al.  Implantation of hydrophilic and hydrophobic titanium discs in rat tibia: cellular reactions on the surfaces during the first 3 weeks in bone. , 2004, Biomaterials.

[23]  D Buser,et al.  What is This? Downloaded from jdr.sagepub.com at PENNSYLVANIA STATE UNIV on February 23, 2013 For personal use only. No other uses without permission. International and American Associations for Dental ResearchRESEARCH REPORTS , 2004 .

[24]  Kwan Tat Steeve,et al.  IL-6, RANKL, TNF-alpha/IL-1: interrelations in bone resorption pathophysiology. , 2004 .

[25]  A. Filippi,et al.  Long-term evaluation of submerged and nonsubmerged ITI solid-screw titanium implants: a 10-year life table analysis of 468 implants. , 2003, The International journal of oral & maxillofacial implants.

[26]  David L. Cochran,et al.  Osteoblasts generate an osteogenic microenvironment when grown on surfaces with rough microtopographies. , 2003, European cells & materials.

[27]  A. Boyde,et al.  Stem Cell Properties of Human Dental Pulp Stem Cells , 2002, Journal of dental research.

[28]  J. Deng,et al.  The Novel Zinc Finger-Containing Transcription Factor Osterix Is Required for Osteoblast Differentiation and Bone Formation , 2002, Cell.

[29]  Thomas D. Schmittgen,et al.  Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. , 2001, Methods.

[30]  B. Boyan,et al.  Inhibition of cyclooxygenase by indomethacin modulates osteoblast response to titanium surface roughness in a time-dependent manner. , 2001, Clinical oral implants research.

[31]  S. Gronthos,et al.  Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo. , 2000, Proceedings of the National Academy of Sciences of the United States of America.

[32]  J. Wozney,et al.  Recombinant human bone morphogenetic protein-2 stimulation of bone formation around endosseous dental implants. , 1999, Journal of periodontology.

[33]  T. Jemt.  A prospective 15-year follow-up study of mandibular fixed prostheses supported by osseointegrated implants , 1997 .

[34]  T Jemt,et al.  A prospective 15-year follow-up study of mandibular fixed prostheses supported by osseointegrated implants. Clinical results and marginal bone loss. , 1996, Clinical oral implants research.

[35]  T. Hirano,et al.  IL-6 is produced by osteoblasts and induces bone resorption. , 1990, Journal of immunology.

[36]  Parthiban,et al.  Bonebiomarkers in periodontal disease: a review article. , 2015, Journal of clinical and diagnostic research : JCDR.

[37]  I. D'alimonte,et al.  Molecular and phenotypic characterization of human amniotic fluid-derived cells: a morphological and proteomic approach. , 2015, Stem cells and development.

[38]  T. Soukup,et al.  Osteogenic differentiation of human dental pulp-derived stem cells under various ex-vivo culture conditions. , 2010, Acta medica.

[39]  D. Prockop,et al.  Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. , 2006, Cytotherapy.

[40]  D. Hurd,et al.  Prostaglandin E2 binding and cyclic AMP production in isolated bone cells , 2006, Calcified Tissue International.

[41]  Anna Tampieri,et al.  Biomimetic Mg- and Mg,CO3-substituted hydroxyapatites: synthesis characterization and in vitro behaviour , 2006 .

[42]  S. Kwan Tat,et al.  IL-6, RANKL, TNF-alpha/IL-1: interrelations in bone resorption pathophysiology. , 2004, Cytokine & growth factor reviews.

[43]  R. Woody,et al.  Implant materials, design, and surface topographies: their influence on osseointegration of dental implants. , 2003, Journal of long-term effects of medical implants.

[44]  M. Sabbieti,et al.  Printed in U.S.A. Copyright © 1999 by The Endocrine Society Prostaglandins Regulate the Expression of Fibroblast Growth Factor-2 in Bone , 2022 .

[45]  J. Davies,et al.  Mechanisms of endosseous integration. , 1998, The International journal of prosthodontics.

[46]  G Zarb,et al.  The long-term efficacy of currently used dental implants: a review and proposed criteria of success. , 1986, The International journal of oral & maxillofacial implants.