Preparation of amino functionalized silica micro beads by dry method for supporting silver nanoparticles with antibacterial properties

Abstract The development of resistance to antibiotics and disinfectants as well as the occurrence of many adverse effects caused by using conventional antibacterial agents like chlorine compounds have prompted scientists to search for new antibacterial agents. Among the many nanomaterials, silver nanoparticles have proven to be a good alternative for its antibacterial properties. In this study, silver nanoparticle-supported silica micro beads (Ag-NPBs) with sizes ranging from 180 to 500 μm have been prepared. Silica gel was synthesized by a sol–gel route and crushed into micro beads, which were modified with 3-aminopropyltriethoxysilane by so-called dry method. Silver nanoparticles were directly created on the surface and in the pores of the modified silica by chemical reduction. The prepared materials were characterized with FT-IR, BET, XRD, FE-SEM, FE-STEM, UV–vis spectrophotometer, and optical microscope. Silver nanoparticles mostly distributed at the outer layer of amino functionalized silica beads. Ag-NPBs samples were examined for their antibacterial properties against Escherichia coli ( E. coli ) and Bacillus subtilis ( B. subtilis ). The excellent antibacterial properties of Ag-NPBs were confirmed with zone inhibition and test tube test. Zone inhibitory tests proved that Ag-NPBs were effective against both gram negative and gram positive bacteria while test tube tests indicated that no E. coli was detected after 1 min contact.

[1]  Chihpin Huang,et al.  Application of nanosilver surface modification to RO membrane and spacer for mitigating biofouling in seawater desalination. , 2009, Water research.

[2]  M. Loessner,et al.  Antimicrobial Properties of a Novel Silver-Silica Nanocomposite Material , 2009, Applied and Environmental Microbiology.

[3]  V. Sharma,et al.  Silver nanoparticles: green synthesis and their antimicrobial activities. , 2009, Advances in colloid and interface science.

[4]  Tetsuaki Tsuchido,et al.  Mode of Bactericidal Action of Silver Zeolite and Its Comparison with That of Silver Nitrate , 2003, Applied and Environmental Microbiology.

[5]  Georgios A Sotiriou,et al.  Nanosilver on nanostructured silica: Antibacterial activity and Ag surface area. , 2011, Chemical engineering journal.

[6]  A. Alanis,et al.  Resistance to antibiotics: are we in the post-antibiotic era? , 2005, Archives of medical research.

[7]  Seong-Geun Oh,et al.  Preparation and antibacterial effects of Ag-SiO2 thin films by sol-gel method. , 2003, Biomaterials.

[8]  D. Chakravorty,et al.  Silver nanowires grown in the pores of a silica gel , 2000 .

[9]  Pradip B. Sarawade,et al.  Facile route for preparation of silver nanoparticle-coated precipitated silica , 2011 .

[10]  Léna Brunet,et al.  Polysulfone ultrafiltration membranes impregnated with silver nanoparticles show improved biofouling resistance and virus removal. , 2009, Water research.

[11]  D. Gangadharan,et al.  Polymeric microspheres containing silver nanoparticles as a bactericidal agent for water disinfection. , 2010, Water research.

[12]  Chang Woo Kim,et al.  Bulklike Thermal Behavior of Antibacterial Ag−SiO2 Nanocomposites , 2009 .

[13]  Pradip B. Sarawade,et al.  Synthesis of hydrophilic and hydrophobic xerogels with superior properties using sodium silicate , 2011 .

[14]  Dae Hong Jeong,et al.  Antimicrobial effects of silver nanoparticles. , 2007, Nanomedicine : nanotechnology, biology, and medicine.

[15]  Seong-Geun Oh,et al.  Facile route for preparation of silica–silver heterogeneous nanocomposite particles using alcohol reduction method , 2007 .

[16]  Siddhartha P Duttagupta,et al.  Strain specificity in antimicrobial activity of silver and copper nanoparticles. , 2008, Acta biomaterialia.

[17]  M. Rai,et al.  Silver nanoparticles as a new generation of antimicrobials. , 2009, Biotechnology advances.

[18]  B. Gibbins,et al.  Antimicrobial surface functionalization of plastic catheters by silver nanoparticles. , 2008, The Journal of antimicrobial chemotherapy.

[19]  K. Vijayamohanan,et al.  Synthesis of silver nanowires inside mesoporous MCM-41 host , 2004 .

[20]  Ping Yin,et al.  Adsorption Kinetics and Isotherms of Ag(I) and Hg(II) onto Silica Gel with Functional Groups of Hydroxyl- or Amino-Terminated Polyamines , 2011 .

[21]  Fengting Li,et al.  Hybrid surfactant-templated mesoporous silica formed in ethanol and its application for heavy metal removal. , 2008, Journal of hazardous materials.

[22]  Stephen W. Howell,et al.  Vapor Sensing Using Conjugated Molecule-Linked Au Nanoparticles in a Silica Matrix , 2009 .

[23]  B. Nagarajan,et al.  Design and Application of Nano Silver Based Pou Appliances for Disinfection of Drinking Water , 2009 .

[24]  K. Nahm,et al.  Nanometer Sized Silver Particles Embedded Silica Particles—Spray Method , 2009, Nanoscale research letters.

[25]  Michael V. Liga,et al.  Virus inactivation by silver doped titanium dioxide nanoparticles for drinking water treatment. , 2011, Water research.

[26]  J. Khinast,et al.  Adsorption of heavy metal cations by organic ligands grafted on porous materials , 2009 .

[27]  K. Fujimoto,et al.  Micro FT-IR study of the hydration-layer during dissolution of silica glass , 1997 .

[28]  H. Guess,et al.  Drinking water and cancer: review of recent epidemiological findings and assessment of risks. , 1982, Annual review of public health.

[29]  Yi Cui,et al.  High speed water sterilization using one-dimensional nanostructures. , 2010, Nano letters.

[30]  M Jacobsen,et al.  Re: Carcinogenicity of the drinking water mutagen 3-chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone in the rat. , 1997, Journal of the National Cancer Institute.

[31]  Angelo Taglietti,et al.  Self-assembled monolayers of silver nanoparticles firmly grafted on glass surfaces: low Ag+ release for an efficient antibacterial activity. , 2010, Journal of Colloid and Interface Science.

[32]  Pradip B. Sarawade,et al.  Preparation of silver nanoparticle containing silica micro beads and investigation of their antibacterial activity , 2011 .

[33]  P. Jain,et al.  Potential of silver nanoparticle-coated polyurethane foam as an antibacterial water filter. , 2005, Biotechnology and bioengineering.

[34]  F. Rubio,et al.  Study of the hydrolysis and condensation of γ-Aminopropyltriethoxysilane by FT-IR spectroscopy , 2007 .

[35]  S. L. Chang,et al.  The safety of water disinfection. , 1982, Annual review of public health.

[36]  T. Kokubo,et al.  Antibacterial activity of silver-doped silica glass microspheres prepared by a sol-gel method. , 2007, Journal of biomedical materials research. Part B, Applied biomaterials.

[37]  James A. Smith,et al.  Sustainable colloidal-silver-impregnated ceramic filter for point-of-use water treatment. , 2008, Environmental science & technology.

[38]  Priyabrata Mukherjee,et al.  Biological properties of "naked" metal nanoparticles. , 2008, Advanced drug delivery reviews.

[39]  Y. Vorobiev,et al.  Infrared spectroscopy analysis of the local atomic structure in silica prepared by sol-gel , 1998 .

[40]  Stephen W. Gundry,et al.  Household drinking water in developing countries: a systematic review of microbiological contamination between source and point‐of‐use , 2004, Tropical medicine & international health : TM & IH.

[41]  A. D. Russell,et al.  Antiseptics and Disinfectants: Activity, Action, and Resistance , 1999, Clinical Microbiology Reviews.

[42]  Jong-Kil Kim,et al.  Synthesis and characterization of nanoporous silica support for enzyme immobilization , 2004 .

[43]  Yuji Naito,et al.  Silver nanoparticles as a safe preservative for use in cosmetics. , 2010, Nanomedicine : nanotechnology, biology, and medicine.

[44]  Hyun Gil Cha,et al.  Synthesis and Characterization of Antibacterial Ag−SiO2 Nanocomposite , 2007 .

[45]  N. V. Trung,et al.  Synthesis of nanosilver particles by reverse micelle method and study of their bactericidal properties , 2009 .

[46]  Michael V. Liga,et al.  Antimicrobial nanomaterials for water disinfection and microbial control: potential applications and implications. , 2008, Water research.

[47]  R. Bandyopadhyaya,et al.  Silver-embedded granular activated carbon as an antibacterial medium for water purification , 2008 .

[48]  K. Chakrabarti,et al.  Silver doped ORMOSIL —an investigation on structural and physical properties , 2002 .

[49]  A. Pourahmad,et al.  Preparation and characterization of Ag nanowires in mesoporous MCM-41 nanoparticles template by chemical reduction method , 2009 .

[50]  H J Klasen,et al.  Historical review of the use of silver in the treatment of burns. I. Early uses. , 2000, Burns : journal of the International Society for Burn Injuries.

[51]  Young‐Kwon Park,et al.  Effect of pore structure of amine-functionalized mesoporous silica-supported rhodium catalysts on 1-octene hydroformylation , 2009 .

[52]  T. Xia,et al.  Particle size and pore structure characterization of silver nanoparticles prepared by confined arc plasma , 2009 .

[53]  K. Paknikar,et al.  Silver nanoparticles in therapeutics: development of an antimicrobial gel formulation for topical use. , 2009, Molecular pharmaceutics.

[54]  Lesile Glasser.  The chemistry of silica: By Ralph K. Iller. Pp. vii+ 866. Wiley, Chichester. 1979, £39.50 , 1980 .

[55]  Hao Li,et al.  Antibacterial activities of zinc oxide nanoparticles against Escherichia coli O157:H7 , 2009, Journal of applied microbiology.

[56]  Yaohui Lv,et al.  Silver nanoparticle-decorated porous ceramic composite for water treatment , 2009 .

[57]  Chang Woo Kim,et al.  Preparation and Antibiotic Property of Ag-SiO2 Nanoparticle , 2007 .

[58]  Jai-koo Park,et al.  The preparation of Ag/mesoporous silica by direct silver reduction and Ag/functionalized mesoporous silica by in situ formation of adsorbed silver , 2007 .

[59]  K. Aoki,et al.  Preparation of Silica Microspheres Containing Ag Nanoparticles , 1998 .

[60]  J. Sonnefeld,et al.  Aminopolysiloxane gels: production and properties , 1996 .