Preparative scale expression of membrane proteins in Escherichia coli-based continuous exchange cell-free systems

[1]  E. Bamberg,et al.  Functional cell-free synthesis of a seven helix membrane protein: in situ insertion of bacteriorhodopsin into liposomes. , 2007, Journal of molecular biology.

[2]  Tae-Wan Kim,et al.  An economical and highly productive cell-free protein synthesis system utilizing fructose-1,6-bisphosphate as an energy source. , 2007, Journal of biotechnology.

[3]  H. Michel,et al.  Functional analysis of cell‐free‐produced human endothelin B receptor reveals transmembrane segment 1 as an essential area for ET‐1 binding and homodimer formation , 2007, The FEBS journal.

[4]  A. Engel,et al.  Cell-free production of G protein-coupled receptors for functional and structural studies. , 2007, Journal of structural biology.

[5]  R. Stroud,et al.  Cell‐free complements in vivo expression of the E. coli membrane proteome , 2007, Protein science : a publication of the Protein Society.

[6]  V. Dötsch,et al.  Preparative scale cell-free expression systems: new tools for the large scale preparation of integral membrane proteins for functional and structural studies. , 2007, Methods.

[7]  A. Spirin,et al.  Continuous-exchange protein-synthesizing systems. , 2007, Methods in molecular biology.

[8]  V. Dötsch,et al.  Cell-free production of integral membrane proteins on a preparative scale. , 2007, Methods in molecular biology.

[9]  V. Dötsch,et al.  Cell‐free expression as an emerging technique for the large scale production of integral membrane protein , 2006, The FEBS journal.

[10]  Efficient generation of insect-based cell-free translation extracts active in glycosylation and signal sequence processing. , 2006, Journal of biotechnology.

[11]  Geun-Hee Hahn,et al.  Production of milligram quantities of recombinant proteins from PCR-amplified DNAs in a continuous-exchange cell-free protein synthesis system. , 2006, Analytical biochemistry.

[12]  Tae-Wan Kim,et al.  Rapid production of milligram quantities of proteins in a batch cell-free protein synthesis system. , 2006, Journal of biotechnology.

[13]  Peter Güntert,et al.  Optimal isotope labelling for NMR protein structure determinations , 2006, Nature.

[14]  J. Ahn,et al.  Cell-free synthesis of recombinant proteins from PCR-amplified genes at a comparable productivity to that of plasmid-based reactions. , 2005, Biochemical and biophysical research communications.

[15]  Volker Dötsch,et al.  Evaluation of detergents for the soluble expression of α‐helical and β‐barrel‐type integral membrane proteins by a preparative scale individual cell‐free expression system , 2005 .

[16]  Volker Dötsch,et al.  Efficient strategy for the rapid backbone assignment of membrane proteins. , 2005, Journal of the American Chemical Society.

[17]  N. Dixon,et al.  Cell‐free synthesis of 15N‐labeled proteins for NMR studies , 2005, IUBMB life.

[18]  T. Kigawa,et al.  Expression of G protein coupled receptors in a cell-free translational system using detergents and thioredoxin-fusion vectors. , 2005, Protein expression and purification.

[19]  Volker Dötsch,et al.  Evaluation of detergents for the soluble expression of alpha-helical and beta-barrel-type integral membrane proteins by a preparative scale individual cell-free expression system. , 2005, The FEBS journal.

[20]  J. Betton,et al.  Cell-free synthesis of a functional ion channel in the absence of a membrane and in the presence of detergent. , 2004, Biochemistry.

[21]  A. Spirin.  High-throughput cell-free systems for synthesis of functionally active proteins. , 2004, Trends in biotechnology.

[22]  M. Jewett,et al.  Mimicking the Escherichia coli cytoplasmic environment activates long‐lived and efficient cell‐free protein synthesis , 2004, Biotechnology and bioengineering.

[23]  T. Danieli,et al.  In vitro synthesis of fully functional EmrE, a multidrug transporter, and study of its oligomeric state. , 2004, Proceedings of the National Academy of Sciences of the United States of America.

[24]  Heinz Rüterjans,et al.  High level cell-free expression and specific labeling of integral membrane proteins. , 2004, European journal of biochemistry.

[25]  Yuichiro Shimada,et al.  Functional Expression and Characterization of a Bacterial Light-harvesting Membrane Protein in Escherichia coli and Cell-free Synthesis Systems , 2004, Bioscience, biotechnology, and biochemistry.

[26]  L. Isaksson,et al.  A codon window in mRNA downstream of the initiation codon where NGG codons give strongly reduced gene expression in Escherichia coli. , 2004, Nucleic acids research.

[27]  Shigeyuki Yokoyama,et al.  Protein expression systems for structural genomics and proteomics. , 2003, Current opinion in chemical biology.

[28]  R G Kim,et al.  Expression-independent consumption of substrates in cell-free expression system from Escherichia coli. , 2000, Journal of biotechnology.

[29]  Dong-Myung Kim,et al.  Prolonging Cell‐Free Protein Synthesis by Selective Reagent Additions , 2000, Biotechnology progress.

[30]  Y. Li,et al.  A modified procedure for fast purification of T7 RNA polymerase. , 1999, Protein expression and purification.

[31]  Dong-Myung Kim,et al.  Prolonging cell-free protein synthesis with a novel ATP regeneration system. , 1999, Biotechnology and bioengineering.

[32]  A. Spirin,et al.  Acetyl phosphate as an energy source for bacterial cell-free translation systems. , 1995, Analytical biochemistry.

[33]  T. Kigawa,et al.  A continuous cell-free protein synthesis system for coupled transcription-translation. , 1991, Journal of biochemistry.

[34]  A. Spirin,et al.  A continuous cell-free translation system capable of producing polypeptides in high yield. , 1988, Science.

[35]  C. Anderson,et al.  Preparation of a cell-free protein-synthesizing system from wheat germ. , 1983, Methods in enzymology.

[36]  G. Zubay,et al.  In vitro synthesis of protein in microbial systems. , 1973, Annual review of genetics.