Effect of cytochalasin B, nocodazole and irradiation on migration and internalization of cells and microspheres in tumor cell spheroids.

The effect of cytochalasin B (CB), nocodazole, and irradiation on the adherence and internalization of 3H-labeled EMT6 spheroid-derived single cells and inert microspheres in unlabeled, intact EMT6 multicellular spheroids has been examined. CB inhibited adhesion and internal migration, whereas nocodazole did not stop adhesion but did prevent later internalization. Treatment of labeled cells with 5, 15 and 25 Gy 250 kV X-rays before adherence did not effect their adherence or later internalization. The same radiation treatments administered to the spheroids either immediately before or after the introduction of unirradiated single cells did not affect adherence, but the depths reached by labeled cells and microspheres were reduced largely because of the consequent reduction in spheroid growth. Microsphere size (9, 15, or 25 microns) and surface charge (negative, or non-ionic) had minimal, if any, effect on the adherence and internalization of these particles.

[1]  S. Newman,et al.  Matrix-driven translocation of cells and nonliving particles. , 1985, Science.

[2]  M. Mareel,et al.  Attachment of mouse fibrosarcoma cells to precultured fragments of embryonic chick heart , 1980, Virchows Archiv. B, Cell pathology including molecular pathology.

[3]  R F Kallman,et al.  Migration and internalization of cells and polystyrene microsphere in tumor cell spheroids. , 1982, Experimental cell research.

[4]  P. Armstrong,et al.  CELL SORTING IN THE PRESENCE OF CYTOCHALASIN B , 1972, The Journal of cell biology.

[5]  R. Sutherland,et al.  A move for the better. , 1994, Environmental health perspectives.

[6]  M. Mareel,et al.  Effect of anticancer agents on invasion of mouse fibrosarcoma cells in vitro. , 1981, Oncology.

[7]  J. Carlsson,et al.  The influence of oxygen on viability and proliferation in cellular spheroids. , 1979, International journal of radiation oncology, biology, physics.

[8]  Conan K. N. Li The glucose distribution in 9l rat brain multicell tumor spheroids and its effect on cell necrosis , 1982, Cancer.

[9]  R. Sutherland,et al.  Growth of multicell spheroids in tissue culture as a model of nodular carcinomas. , 1971, Journal of the National Cancer Institute.

[10]  L. F. Fajardo,et al.  Characteristics of a serially transplanted mouse mammary tumor and its tissue-culture-adapted derivative. , 1972, Journal of the National Cancer Institute.

[11]  M. Bronner‐Fraser Distribution of latex beads and retinal pigment epithelial cells along the ventral neural crest pathway. , 1982, Developmental biology.