Biofilm formation on surfaces of glass and Teflon exposed to treated water

Abstract Biofilm formation on glass and Teflon cylinders, situated on top of each other in vertical glass columns exposed to different types of treated water at a flow rate of 0.2 m/s, was determined using ATP analysis and heterotrophic plate counts. Biofilm formation rates (BFR) of these water types, as calculated from the linear relationship between exposure time and biomass accumulated on the cylinder (glass) surface, were 11.1 ± 0.7 pg ATP/cm 2 · d ( r = 0.96) for treated ground water (TGW), 38.2 ± 4.7 pg ATP/cm 2 · d ( r = 0.90) for treated river water (TRW) and 382 ± 30 pg ATP/cm 2 · d ( r = 0.94) for TGW supplemented with 100 μg of acetate-C/1, respectively. Maximum levels of accumulated biomass were 1300 pg ATP/cm 2 after 114 d of exposure for TGW, 3200 pg ATP/cm 2 after 78 d of exposure for TRW and 26,000 pg ATP/cm 2 after 65 d of exposure for TGW with acetate, respectively. Material and position of the cylinder in the columns either had no significant or a small effect on biofilm formation, depending on the water type. The effect of acetate addition on the BFR of TGW clearly demonstrated that biofilm formation was energy-source limited in this water type. These observations show that the experimental setup is suited for assessing BFRs of treated water.

[1]  P. Servais,et al.  Determination of the biodegradable fraction of dissolved organic matter in waters , 1987 .

[2]  Edwin E. Geldreich,et al.  The occurrence of microorganisms in water main encrustations , 1980 .

[3]  A. E. Greenberg,et al.  Standard methods for the examination of water and wastewater : supplement to the sixteenth edition , 1988 .

[4]  Karsten Pedersen,et al.  Biofilm development on stainless steel and PVC surfaces in drinking water , 1990 .

[5]  E. L. Motta.  Kinetics of Growth and Substrate Uptake in a Biological Film System , 1976 .

[6]  Vernon L. Snoeyink,et al.  Achieving Biologically Stable Drinking Water , 1984 .

[7]  E. Carrington,et al.  An evaluation of procedures for enumerating bacteria in activated sludge. , 1972, The Journal of applied bacteriology.

[8]  P. Werner.  Eine Methode zur Bestimmung der Verkeimungsneigung von Trinkwasser , 1985 .

[9]  B H Olson,et al.  Scanning electron microscope evidence for bacterial colonization of a drinking-water distribution system , 1981, Applied and environmental microbiology.

[10]  Wesley O. Pipes,et al.  Selected drinking water characteristics and attached microbial population density , 1988 .

[11]  D. Kooij.  Assimilable Organic Carbon as an Indicator of Bacterial Regrowth , 1992 .

[12]  G. Izaguirre,et al.  Ammonia-oxidizing bacteria in a chloraminated distribution system: seasonal occurrence, distribution and disinfection resistance , 1990, Applied and environmental microbiology.

[13]  John T. Wilson,et al.  Determination of Microbial Cell Numbers in Subsurface Samples , 1985 .

[14]  Dirk van der Kooij,et al.  Determining the concentration of easily assimilable organic carbon in drinking water , 1982 .

[15]  J. Costerton,et al.  Growth of Sessile Sphaerotilus natans in a Tubular Recycle System , 1982, Applied and environmental microbiology.

[16]  William G. Characklis,et al.  Biofilms and bacterial drinking water quality , 1989 .

[17]  Ramon G. Lee,et al.  Disinfecting Biofilms in a Model Distribution System , 1990 .

[18]  E. Geldreich,et al.  A new medium for the enumeration and subculture of bacteria from potable water , 1985, Applied and environmental microbiology.

[19]  M. Lechevallier,et al.  Examination and characterization of distribution system biofilms , 1987, Applied and environmental microbiology.