Label‐Free Ultrasensitive Detection of Human Telomerase Activity Using Porphyrin‐Functionalized Graphene and Electrochemiluminescence Technique

Using porphyrin-functionalized graphene, we construct a PCR-free, low-cost, rapid, and electrochemiluminenscence (ECL) assay for detection of telomerase activity that has been demonstrated in six different cell lines and can be used as initial screening of G-quadruplex DNA binding agents and telomerase inhibitors. This ECL sensor shows highly sensitive for detection of telomerase with the detection limit as low as 10 HeLa cells mL(-1) .

[1]  X. Qu,et al.  DNA loop sequence as the determinant for chiral supramolecular compound G-quadruplex selectivity. , 2010, Journal of medicinal chemistry.

[2]  Hongying Liu,et al.  Electrogenerated chemiluminescence of Au nanoclusters for the detection of dopamine. , 2011, Analytical chemistry.

[3]  David M. Prescott,et al.  Inhibition of telomerase by G-quartet DMA structures , 1991, Nature.

[4]  Genxi Li,et al.  PCR-free electrochemical assay of telomerase activity , 2008 .

[5]  Jing Wang,et al.  Gold nanoparticle enhanced electrochemiluminescence of CdS thin films for ultrasensitive thrombin detection. , 2011, Analytical chemistry.

[6]  H. Dai,et al.  Highly conducting graphene sheets and Langmuir-Blodgett films. , 2008, Nature nanotechnology.

[7]  G. Wallace,et al.  Processable aqueous dispersions of graphene nanosheets. , 2008, Nature nanotechnology.

[8]  Da Xing,et al.  Magnetic bead and nanoparticle based electrochemiluminescence amplification assay for direct and sensitive measuring of telomerase activity. , 2009, Analytical chemistry.

[9]  Itamar Willner,et al.  Catalytic beacons for the detection of DNA and telomerase activity. , 2004, Journal of the American Chemical Society.

[10]  Li Wu,et al.  Visualizing human telomerase activity with primer-modified Au nanoparticles. , 2012, Small.

[11]  B. Shu,et al.  A quantitative method to measure telomerase activity by bioluminescence connected with telomeric repeat amplification protocol. , 2001, Analytical biochemistry.

[12]  D. Langley,et al.  Selective interactions of cationic porphyrins with G-quadruplex structures. , 2001, Journal of the American Chemical Society.

[13]  Yuyan Shao,et al.  Nitrogen-doped graphene and its application in electrochemical biosensing. , 2010, ACS nano.

[14]  M. Ozsoz,et al.  Label-free detection of telomerase activity using guanine electrochemical oxidation signal. , 2007, Analytical chemistry.

[15]  E. Blackburn,et al.  Structure and function of telomeres , 1991, Nature.

[16]  K. Fujita,et al.  Telomerase activity as an indicator of potentially malignant adrenal tumors , 1998, Cancer.

[17]  Guonan Chen,et al.  Signal-on electrochemiluminescent biosensor for ATP based on the recombination of aptamer chip. , 2011, Chemical communications.

[18]  K. Shin‐ya,et al.  Reevaluation of telomerase inhibition by quadruplex ligands and their mechanisms of action , 2007, Proceedings of the National Academy of Sciences.

[19]  Andrew T. Csordas,et al.  Detection of telomerase activity in high concentration of cell lysates using primer-modified gold nanoparticles. , 2010, Journal of the American Chemical Society.

[20]  C B Harley,et al.  Specific association of human telomerase activity with immortal cells and cancer. , 1994, Science.

[21]  Guobao Xu,et al.  Applications and trends in electrochemiluminescence. , 2010, Chemical Society reviews.

[22]  M. Droller,et al.  Detecting human bladder carcinoma cells in voided urine samples by assaying for the presence of telomerase activity , 1998, Cancer.

[23]  H. Soh,et al.  Two‐Step, PCR‐Free Telomerase Detection by Using Exonuclease III‐Aided Target Recycling , 2011, Chembiochem : a European journal of chemical biology.

[24]  N. Mohanty,et al.  Graphene-based single-bacterium resolution biodevice and DNA transistor: interfacing graphene derivatives with nanoscale and microscale biocomponents. , 2008, Nano letters.

[25]  Tae Seok Seo,et al.  A graphene oxide based immuno-biosensor for pathogen detection. , 2010, Angewandte Chemie.

[26]  Jun‐Jie Zhu,et al.  Fabrication of Graphene–Quantum Dots Composites for Sensitive Electrogenerated Chemiluminescence Immunosensing , 2011 .

[27]  Jie Wu,et al.  Ultrasensitive immunoassay of protein biomarker based on electrochemiluminescent quenching of quantum dots by hemin bio-bar-coded nanoparticle tags. , 2011, Analytical chemistry.

[28]  X. Qu,et al.  A graphene functionalized electrochemical aptasensor for selective label-free detection of cancer cells. , 2011, Biomaterials.

[29]  R. Kaner,et al.  Honeycomb carbon: a review of graphene. , 2010, Chemical reviews.

[30]  Chunhai Fan,et al.  Graphene oxide-facilitated electron transfer of metalloproteins at electrode surfaces. , 2010, Langmuir : the ACS journal of surfaces and colloids.

[31]  W. Haiyan,et al.  Study on the Intercalation and Interlayer State of Porphyrins into α-Zirconium Phosphate , 2005 .

[32]  X. Qu,et al.  Chiral metallo-supramolecular complexes selectively induce human telomeric G-quadruplex formation under salt-deficient conditions. , 2011, Chemistry.

[33]  Jin-Young Park,et al.  DNA Hybridization Sensors Based on Electrochemical Impedance Spectroscopy as a Detection Tool , 2009, Sensors.

[34]  K. Ohuchida,et al.  A highly sensitive and quantitative telomerase activity assay with pancreatic juice is useful for diagnosis of pancreatic carcinoma without problems due to polymerase chain reaction inhibitors , 2004, Cancer.

[35]  H. Park,et al.  An ultrasensitive DNAzyme-based colorimetric strategy for nucleic acid detection. , 2009, Chemical communications.

[36]  H. Ju,et al.  Characterization, direct electrochemistry, and amperometric biosensing of graphene by noncovalent functionalization with picket-fence porphyrin. , 2010, Chemistry.

[37]  Itamar Willner,et al.  DNAzyme-Functionalized Au Nanoparticles for the Amplified Detection of DNA or Telomerase Activity , 2004 .

[38]  B. Alpha-Bazin,et al.  A homogeneous time-resolved fluorescence detection of telomerase activity. , 2004, Analytical biochemistry.

[39]  A. Govindaraj,et al.  Graphene: the new two-dimensional nanomaterial. , 2009, Angewandte Chemie.

[40]  Zhenyu Lin,et al.  Label-free detection of telomerase activity in HeLa cells using electrochemical impedance spectroscopy. , 2011, Chemical communications.

[41]  X. Qu,et al.  Colorimetric Biosensing Using Smart Materials , 2011, Advanced materials.

[42]  Zhuang Liu,et al.  PEGylated nanographene oxide for delivery of water-insoluble cancer drugs. , 2008, Journal of the American Chemical Society.

[43]  X. Qu,et al.  Chiral metallo-supramolecular complexes selectively recognize human telomeric G-quadruplex DNA , 2008, Nucleic acids research.

[44]  Min Han,et al.  Facile synthesis of PbSe hollow nanostructure assemblies via a solid/liquid-phase chemical route and their electrogenerated chemiluminescence properties. , 2011, Chemistry.

[45]  N. Sugimoto,et al.  Detection of a Prognostic Indicator in Early‐Stage Cancer Using Functionalized Graphene‐Based Peptide Sensors , 2012, Advanced materials.

[46]  Yi Xiao,et al.  Amplified chemiluminescence surface detection of DNA and telomerase activity using catalytic nucleic acid labels. , 2004, Analytical chemistry.

[47]  E. Raymond,et al.  Effects of cationic porphyrins as G-quadruplex interactive agents in human tumor cells. , 1999, Cancer research.

[48]  H. Kondo,et al.  Electrochemical telomerase assay with ferrocenylnaphthalene diimide as a tetraplex DNA-specific binder. , 2005, Analytical chemistry.

[49]  Ken‐ichiro Yoshida,et al.  Telomerase activity in renal cell carcinoma , 1998, Cancer.

[50]  R. Yu,et al.  Graphene oxide-peptide conjugate as an intracellular protease sensor for caspase-3 activation imaging in live cells. , 2011, Angewandte Chemie.

[51]  Ying Li,et al.  Formation of G-quadruplex-hemin DNAzyme based on human telomere elongation and its application in telomerase activity detection. , 2011, Biosensors & bioelectronics.

[52]  Ying Li,et al.  Highly sensitive electrochemical detection of human telomerase activity based on bio-barcode method. , 2010, Biosensors & bioelectronics.

[53]  W. Klapper,et al.  Molecular basis of artifacts in the detection of telomerase activity and a modified primer for a more robust 'TRAP' assay. , 1997, Nucleic acids research.

[54]  et al.,et al.  The RNA component of human telomerase , 1995, Science.

[55]  I. Willner,et al.  Magneto-mechanical detection of nucleic acids and telomerase activity in cancer cells. , 2004, Journal of the American Chemical Society.

[56]  Xiaogang Qu,et al.  Graphene Oxide: Intrinsic Peroxidase Catalytic Activity and Its Application to Glucose Detection , 2010, Advanced materials.

[57]  A. Bard,et al.  Immobilization and Hybridization of DNA on an Aluminum(III) Alkanebisphosphonate Thin Film with Electrogenerated Chemiluminescent Detection , 1995 .

[58]  I. Willner,et al.  DNAzyme‐Like Activity of Hemin–Telomeric G‐Quadruplexes for the Optical Analysis of Telomerase and its Inhibitors , 2010, Chembiochem : a European journal of chemical biology.

[59]  M. Pumera.  Graphene-based nanomaterials and their electrochemistry. , 2010, Chemical Society reviews.

[60]  M. Pumera,et al.  Graphene platform for hairpin-DNA-based impedimetric genosensing. , 2011, ACS nano.

[61]  Ram Datar,et al.  Rapid, label-free, electrical whole blood bioassay based on nanobiosensor systems. , 2011, ACS nano.

[62]  H. Postma,et al.  Rapid sequencing of individual DNA molecules in graphene nanogaps. , 2008, Nano letters.