A focused antibody library for selecting scFvs expressed at high levels in the cytoplasm

[1]  Rodrigo Barderas,et al.  A fast mutagenesis procedure to recover soluble and functional scFvs containing amber stop codons from synthetic and semisynthetic antibody libraries. , 2006, Journal of immunological methods.

[2]  Patrice Duroux,et al.  IMGT/LIGM-DB, the IMGT® comprehensive database of immunoglobulin and T cell receptor nucleotide sequences , 2005, Nucleic Acids Res..

[3]  L. Wyns,et al.  Identification of a universal VHH framework to graft non-canonical antigen-binding loops of camel single-domain antibodies. , 2005, Journal of molecular biology.

[4]  H. Hoogenboom,et al.  Selecting and screening recombinant antibody libraries , 2005, Nature Biotechnology.

[5]  A. Merlo,et al.  Design, construction, and characterization of a large synthetic human antibody phage display library , 2005, Proteomics.

[6]  Etienne Weiss,et al.  Extended half‐life upon binding of destabilized intrabodies allows specific detection of antigen in mammalian cells , 2005, The FEBS journal.

[7]  C. J. Bond,et al.  A structure-based database of antibody variable domain diversity. , 2005, Journal of molecular biology.

[8]  Wei Wang,et al.  Progress in the development and application of computational methods for probabilistic protein design , 2005, Comput. Chem. Eng..

[9]  P. Martineau,et al.  Directed evolution of single-chain Fv for cytoplasmic expression using the β-galactosidase complementation assay results in proteins highly susceptible to protease degradation and aggregation , 2004, Microbial cell factories.

[10]  M. Quondam,et al.  The intracellular antibody capture technology: towards the high-throughput selection of functional intracellular antibodies for target validation. , 2004, Methods.

[11]  A. Barberis,et al.  Antigen-independent selection of intracellular stable antibody frameworks. , 2004, Methods.

[12]  R. Kontermann.  Intrabodies as therapeutic agents. , 2004, Methods.

[13]  C. Eigenbrot,et al.  High-affinity human antibodies from phage-displayed synthetic Fab libraries with a single framework scaffold. , 2004, Journal of molecular biology.

[14]  Noreen R. Gonzales,et al.  SDR grafting of a murine antibody using multiple human germline templates to minimize its immunogenicity. , 2004, Molecular immunology.

[15]  A. Cattaneo,et al.  Intracellular antibodies for proteomics☆ , 2004, Journal of Immunological Methods.

[16]  Frederic A. Fellouse,et al.  Phage-displayed antibody libraries of synthetic heavy chain complementarity determining regions. , 2004, Journal of molecular biology.

[17]  J. Engler,et al.  Expressed murine and human CDR-H3 intervals of equal length exhibit distinct repertoires that differ in their amino acid composition and predicted range of structures. , 2003, Journal of molecular biology.

[18]  Anna Tramontano,et al.  Engineering stable cytoplasmic intrabodies with designed specificity. , 2003, Journal of molecular biology.

[19]  P. Martineau,et al.  Nucleocytoplasmic shuttling of antigen in mammalian cells conferred by a soluble versus insoluble single-chain antibody fragment equipped with import/export signals. , 2003, Experimental cell research.

[20]  B. Goud,et al.  Recombinant Antibodies to the Small GTPase Rab6 as Conformation Sensors , 2003, Science.

[21]  T. Rabbitts,et al.  Intrabodies based on intracellular capture frameworks that bind the RAS protein with high affinity and impair oncogenic transformation , 2003, The EMBO journal.

[22]  B. Clark,et al.  Identification of Keratinocyte-specific Markers Using Phage Display and Mass Spectrometry* , 2003, Molecular & Cellular Proteomics.

[23]  Andrew C. R. Martin,et al.  Analysis of the antigen combining site: correlations between length and sequence composition of the hypervariable loops and the nature of the antigen. , 2003, Journal of molecular biology.

[24]  Annemarie Honegger,et al.  Direct in Vivo Screening of Intrabody Libraries Constructed on a Highly Stable Single-chain Framework* , 2002, The Journal of Biological Chemistry.

[25]  Wei Wang,et al.  Designing gene libraries from protein profiles for combinatorial protein experiments. , 2002, Nucleic acids research.

[26]  P. Martineau,et al.  Expression and folding of an antibody fragment selected in vivo for high expression levels in Escherichia coli cytoplasm. , 2002, Research in microbiology.

[27]  A. Cattaneo,et al.  Intracellular Single-Chain Variable Fragments Directed to the Src Homology 2 Domains of Syk Partially Inhibit FcεRI Signaling in the RBL-2H3 Cell Line1 , 2002, The Journal of Immunology.

[28]  A. Maritan,et al.  The intracellular antibody capture technology (IACT): towards a consensus sequence for intracellular antibodies. , 2002, Journal of molecular biology.

[29]  Y. Berdichevsky,et al.  Escherichia coli maltose-binding protein as a molecular chaperone for recombinant intracellular cytoplasmic single-chain antibodies. , 2001, Journal of molecular biology.

[30]  R. Chiaraluce,et al.  A semi-synthetic repertoire of intrinsically stable antibody fragments derived from a single-framework scaffold. , 2001, Journal of molecular biology.

[31]  Volker Sieber,et al.  Libraries of hybrid proteins from distantly related sequences , 2001, Nature Biotechnology.

[32]  E. Weiss,et al.  Production of fluorescent single-chain antibody fragments in Escherichia coli. , 2000, Protein expression and purification.

[33]  G. Georgiou,et al.  Quantitative analysis of the effect of the mutation frequency on the affinity maturation of single chain Fv antibodies. , 2000, Proceedings of the National Academy of Sciences of the United States of America.

[34]  A. Plückthun,et al.  Fully synthetic human combinatorial antibody libraries (HuCAL) based on modular consensus frameworks and CDRs randomized with trinucleotides. , 2000, Journal of molecular biology.

[35]  H R Hoogenboom,et al.  Diverting a protein from its cellular location by intracellular antibodies. The case of p21Ras. , 2000, European journal of biochemistry.

[36]  A. Plückthun,et al.  Correlation between in Vitro Stability and in Vivo Performance of Anti-GCN4 Intrabodies as Cytoplasmic Inhibitors* , 2000, The Journal of Biological Chemistry.

[37]  T. Rabbitts,et al.  Selection of antibodies for intracellular function using a two-hybrid in vivo system. , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[38]  P. Martineau,et al.  In vitro folding and thermodynamic stability of an antibody fragment selected in vivo for high expression levels in Escherichia coli cytoplasm. , 1999, Journal of molecular biology.

[39]  Haruki Nakamura,et al.  H3‐rules: identification of CDR‐H3 structures in antibodies , 1999, FEBS letters.

[40]  A. Plückthun,et al.  Removal of the conserved disulfide bridges from the scFv fragment of an antibody: effects on folding kinetics and aggregation. , 1999, Journal of molecular biology.

[41]  T. Terwilliger,et al.  Rapid protein-folding assay using green fluorescent protein , 1999, Nature Biotechnology.

[42]  G. Winter,et al.  Proteolytic selection for protein folding using filamentous bacteriophages. , 1998, Folding & design.

[43]  P. Martineau,et al.  Expression of an antibody fragment at high levels in the bacterial cytoplasm. , 1998, Journal of molecular biology.

[44]  A. Plückthun,et al.  Improving in vivo folding and stability of a single-chain Fv antibody fragment by loop grafting. , 1997, Protein engineering.

[45]  Andrew J. Martin,et al.  Antibody-antigen interactions: contact analysis and binding site topography. , 1996, Journal of molecular biology.

[46]  A. Cattaneo,et al.  Redox State of Single Chain Fv Fragments Targeted to the Endoplasmic Reticulum, Cytosol and Mitochondria , 1995, Bio/Technology.

[47]  J. Link,et al.  Selection and rapid purification of murine antibody fragments that bind a transition-state analog by phage display , 1994, Applied biochemistry and biotechnology.

[48]  S. Garges.  A Short Course in Bacterial Genetics. A Laboratory Manual and Handbook for Escherichia coli and Related Bacteria. By Jeffrey H. Miller. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, 1992. , 1993 .

[49]  Brad Snedecor,et al.  High Level Escherichia coli Expression and Production of a Bivalent Humanized Antibody Fragment , 1992, Bio/Technology.

[50]  M. Neuberger,et al.  Expression and targeting of intracellular antibodies in mammalian cells. , 1990, The EMBO journal.

[51]  R. Sauer,et al.  The structural stability of a protein is an important determinant of its proteolytic susceptibility in Escherichia coli. , 1989, The Journal of biological chemistry.

[52]  J. Carlson,et al.  A new means of inducibly inactivating a cellular protein , 1988, Molecular and cellular biology.

[53]  S. Green,et al.  A versatile in vivo and in vitro eukaryotic expression vector for protein engineering , 1988, Nucleic Acids Res..

[54]  R. Jemmerson.  Multiple overlapping epitopes in the three antigenic regions of horse cytochrome c1. , 1987, Journal of immunology.

[55]  S. Munro,et al.  An hsp70-like protein in the ER: Identity with the 78 kd glucose-regulated protein and immunoglobulin heavy chain binding protein , 1986, Cell.

[56]  C. Chothia,et al.  Domain association in immunoglobulin molecules. The packing of variable domains. , 1985, Journal of molecular biology.

[57]  P. Kristensen,et al.  Isolation of recombinant phage-displayed antibodies recognizing skin keratinocytes. , 2005, Methods in molecular biology.

[58]  J. Marks.  Antibody affinity maturation by chain shuffling. , 2004, Methods in molecular biology.

[59]  Andrew C. R. Martin,et al.  SACS-Self-maintaining database of antibody crystal structure information , 2002, Bioinform..

[60]  S. Sidhu,et al.  Phage display for selection of novel binding peptides. , 2000, Methods in enzymology.

[61]  T. N. Bhat,et al.  The Protein Data Bank , 2000, Nucleic Acids Res..

[62]  George Johnson,et al.  Kabat Database and its applications: 30 years after the first variability plot , 2000, Nucleic Acids Res..

[63]  A. Mittermaier,et al.  A simple in vivo assay for increased protein solubility , 1999, Protein science : a publication of the Protein Society.

[64]  G. Winter,et al.  Screening of phage antibody libraries. , 1996, Methods in enzymology.

[65]  Jeffrey H. Miller.  A Short Course in Bacterial Genetics: A Laboratory Manual and Handbook for Escherichia coli and Rela , 1992 .

[66]  J. Lee,et al.  Preparation of tubulin from brain. , 1982, Methods in enzymology.

[67]  James C. Lee,et al.  [36] Preparation of tubulin from brain , 1982 .