Tridimensional structure of the Golgi apparatus in type A ganglion cells of the rat.

The three-dimensional structure of the whole Golgi apparatus and of its components in type A ganglion cells was examined in thin and thick sections by low- and high-voltage electron microscopy. At low magnification, in 10-micron-thick sections of osmicated cells, the Golgi apparatus formed a broad, continuous perinuclear network. At higher magnification and in thinner sections of cells impregnated with uranyl acetate-lead-copper citrate or postfixed in K-ferrocyanide-reduced osmium, the Golgi apparatus appeared as a heterogeneous structure in which saccular regions characterized by stacks of saccules alternated with intersaccular regions made up of branching membranous tubules which bridged the saccules of adjacent stacks. The saccular regions consisted of the following superimposed elements: a cis-osmiophilic element made up of anastomosing tubules; two or three saccules negative for the phosphatases tested (i.e., nicotinamide adenine dinucleotide phosphatase = NADPase, thiamine pyrophosphatase = TPPase, and cytidine monophosphatase = CMPase); two saccules showing TPPase activity; and one to three trans-sacculotubular elements showing a "peeling-off" configuration, one of which showed CMPase activity. The saccules (phosphatase-negative) on the cis-side of the Golgi stacks showed, in addition to small circular pores, larger perforations in register. The cavities thus formed in the stacks of saccules, called "wells," always associated with small 80-nm vesicles, had a pan shape with the mouth directed toward the cis-face and the bottom closed by a TPPase-positive saccule. In face views of the saccules, the smallest of these perforations showed either a crescent shape, due to the presence of a bud on one side of the perforation, or a circular shape with a single small 80-nm vesicle in the center which was occasionally attached to the saccule by a filiform stalk. Such smaller cavities were considered as the precursors of the larger perforations and eventually of the wells. The small 80-nm vesicles seen in the small cavities or in the wells appeared to form in situ and possibly migrate toward the cisternae of endoplasmic reticulum seen proximal to the cis-face of the stack of saccules. Small 80-nm vesicles were also numerous in the intersaccular regions, along the lateral- and trans-aspects of the Golgi stacks, while larger, 150-to 300-nm vesicles, coated and uncoated, were seen only on the trans-face of the Golgi stacks in proximity to the trans-sacculotubular elements which appear to "peel off" from the Golgi stacks.

[1]  J. Rothman,et al.  Intercompartmental transport in the Golgi complex is a dissociative process: facile transfer of membrane protein between two Golgi populations , 1984, The Journal of cell biology.

[2]  D. Segretain,et al.  Tridimensional architecture of the Golgi apparatus in the atrial muscle cell of the rat. , 1984, The American journal of anatomy.

[3]  A. Hand,et al.  The Golgi apparatus and GERL during postnatal differentiation of rat parotid acinar cells: an electron microscopic cytochemical study. , 1984, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[4]  A. Hand,et al.  Effects of secretory stimulation on the Golgi apparatus and GERL of rat parotid acinar cells. , 1984, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[5]  K. Ogawa,et al.  GOLGI APPARATUS IS ONE CONTINUOUS ORGANELLE IN PANCREATIC EXOCRINE CELL OF MOUSE , 1984 .

[6]  C. P. Leblond,et al.  Collagen biogenesis and assembly into fibrils as shown by ultrastructural and 3H-proline radioautographic studies on the fibroblasts of the rat food pad. , 1983, The American journal of anatomy.

[7]  A. Hand,et al.  Enzyme modulation of the Golgi apparatus and GERL: a cytochemical study of parotid acinar cells. , 1983, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[8]  A. Beaudet,et al.  Ultrastructural features of six types of neurons in rat dorsal root ganglia , 1983, Journal of neurocytology.

[9]  S. Goldfischer The internal reticular apparatus of Camillo Golgi: a complex, heterogeneous organelle, enriched in acid, neutral, and alkaline phosphatases, and involved in glycosylation, secretion, membrane flow, lysosome formation, and intracellular digestion. , 1982, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[10]  M. Ichikawa,et al.  High resolution analysis of three-dimensional structure of the Golgi apparatus in rapid-frozen, substitution fixed gerbil sublingual gland acinar cells. , 1982, Journal of electron microscopy.

[11]  M. Lalli,et al.  Ultrastructural localization of nicotinamide adenine dinucleotide phosphatase (NADPase), thiamine pyrophosphatase (TPPase), and cytidine monophosphatase (CMPase) in the Golgi apparatus of early spermatids of the rat , 1981, The Anatomical record.

[12]  G. Palade,et al.  The Golgi apparatus (complex)-(1954-1981)-from artifact to center stage , 1981, The Journal of cell biology.

[13]  R. Broadwell,et al.  Golgi apparatus, GERL, and secretory granule formation within neurons of the hypothalamo-neurohypophysial system of control and hyperosmotically stressed mice , 1981, The Journal of cell biology.

[14]  L. Hermo,et al.  Chapter 11 Three-Dimensional Structure of the Golgi Apparatus , 1981 .

[15]  L. Hermo,et al.  Three-dimensional architecture of the cortical region of the Golgi apparatus in rat spermatids. , 1980, The American journal of anatomy.

[16]  C. E. Smith Ultrastructural localization of nicotinamide adenine dinucleotide phosphatase (NADPase) activity to the intermediate saccules of the Golgi apparatus in rat incisor ameloblasts. , 1980, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[17]  L. Hermo,et al.  Three-dimensional architecture of the golgi apparatus in Sertoli cells of the rat. , 1979, The American journal of anatomy.

[18]  L. Hermo,et al.  Endoplasmic reticulum‐Golgi apparatus relationships in the rat spermatid , 1979, The Anatomical record.

[19]  M. Mori,et al.  Studies of the secretory process in the mammalian exocrine pancreas. I. The condensing vacuoles , 1977, The Journal of cell biology.

[20]  Kinji Inoue,et al.  Cytochemical and Three-Dimensional Studies on Golgi Apparatus and GERL of Rat Anterior Pituitary Cells by Transmission Electron Microscopy , 1977 .

[21]  G. Palade,et al.  Intracellular aspects of the process of protein synthesis. , 1975, Science.

[22]  Y. Clermont,et al.  Three-dimensional structure of the osmium-impregnated Golgi apparatus as seen in the high voltage electron microscope. , 1974, The American journal of anatomy.

[23]  J. Hauw,et al.  GOLGI APPARATUS, GERL, AND LYSOSOMES OF NEURONS IN RAT DORSAL ROOT GANGLIA, STUDIED BY THICK SECTION AND THIN SECTION CYTOCHEMISTRY , 1971, The Journal of cell biology.

[24]  C. P. Leblond,et al.  Changes in the golgi apparatus during spermiogenesis in the rat. , 1971, The American journal of anatomy.

[25]  N. Carasso,et al.  Observation, en microscopie électronique haute tension, de l'appareil de Golgi sur coupes de 0,5 á 5 microns d'épaisseur. , 1971 .

[26]  D. Friend,et al.  OSMIUM IMPREGNATION OF THE GOLGI APPARATUS. , 1965, The American journal of anatomy.

[27]  D. Friend THE FINE STRUCTURE OF BRUNNER'S GLANDS IN THE MOUSE , 1965, The Journal of cell biology.

[28]  G. Palade,et al.  PROTEIN SYNTHESIS, STORAGE, AND DISCHARGE IN THE PANCREATIC EXOCRINE CELL , 1964, The Journal of cell biology.

[29]  A. Novikoff,et al.  Nucleosidediphosphatase activity in the Golgi apparatus and its usefulness for cytological studies. , 1961, Proceedings of the National Academy of Sciences of the United States of America.