Bioconversion of oxygen-pretreated Kraft lignin to microbial lipid with oleaginous Rhodococcus opacus DSM 1069

Kraft lignin (KL) from black liquor is an abundantly available, inexpensive aromatic resource that is regarded as a low value compound by the pulp and paper industry, necessitating the development of new applications. Current work proposes an innovative KL utilization strategy that connects partial lignin degradation with lipid production in oleaginous Rhodococcus. Results showed poor bacterial growth when KL was used directly as a substrate. On the other hand, when KL recalcitrance was reduced by oxygen-pretreatment (O2-KL), Rhodococcus opacus DSM 1069 was capable of utilizing this material and in turn accumulated lipids. The maximum lipid yield was measured to be 0.067 mg ml−1 at 36 h of growth and these lipids were mainly composed of palmitic (46.9%) and stearic (42.7%) acids.

[1]  J. Coon,et al.  Formic-acid-induced depolymerization of oxidized lignin to aromatics , 2014, Nature.

[2]  A. Ragauskas,et al.  Pyrolysis Oil-Based Lipid Production as Biodiesel Feedstock by Rhodococcus opacus , 2014, Applied Biochemistry and Biotechnology.

[3]  A. Ragauskas,et al.  Polymerization of Kraft lignin via ultrasonication for high-molecular-weight applications. , 2013, Ultrasonics sonochemistry.

[4]  Ryan J. Stoklosa,et al.  Correlating lignin structural features to phase partitioning behavior in a novel aqueous fractionation of softwood Kraft black liquor , 2013 .

[5]  A. Ragauskas,et al.  Lignin to lipid bioconversion by oleaginous Rhodococci , 2013 .

[6]  Lu Lin,et al.  Characterization of Structural Changes of Lignin in the Process of Cooking of Bagasse with Solid Alkali and Active Oxygen as a Pretreatment for Lignin Conversion , 2012 .

[7]  A. Ragauskas,et al.  Kraft Lignin-Based Rigid Polyurethane Foam , 2012 .

[8]  J. Nam,et al.  An eco-friendly and efficient route of lignin extraction from black liquor and a lignin-based copolyester synthesis , 2012, Polymer Bulletin.

[9]  A. Ragauskas,et al.  Bioconversion of lignin model compounds with oleaginous Rhodococci , 2011, Applied Microbiology and Biotechnology.

[10]  E. Hardiman,et al.  Pathways for degradation of lignin in bacteria and fungi. , 2011, Natural product reports.

[11]  Francesc Ferrando,et al.  Kraft lignin behavior during reaction in an alkaline medium , 2011 .

[12]  A. Ragauskas,et al.  Lipids from heterotrophic microbes: advances in metabolism research. , 2011, Trends in biotechnology.

[13]  F. G. Calvo-Flores,et al.  Lignin as renewable raw material. , 2010, ChemSusChem.

[14]  A. Sinskey,et al.  High-cell-density batch fermentation of Rhodococcus opacus PD630 using a high glucose concentration for triacylglycerol production. , 2010, Journal of biotechnology.

[15]  A. Ragauskas,et al.  Characterization of CO2 precipitated Kraft lignin to promote its utilization , 2010 .

[16]  A. Ragauskas,et al.  Characterization of milled wood lignin and ethanol organosolv lignin from miscanthus , 2009 .

[17]  Chao Huang,et al.  Microbial oil production from rice straw hydrolysate by Trichosporon fermentans. , 2009, Bioresource technology.

[18]  M. Zong,et al.  Efficient lipid production with Trichosporon fermentans and its use for biodiesel preparation. , 2008, Bioresource technology.

[19]  M. K. Gouda,et al.  Single cell oil production by Gordonia sp. DG using agro-industrial wastes , 2008 .

[20]  H. Sahm,et al.  Degradation of coniferyl alcohol and other lignin-related aromatic compounds by Nocardia sp. DSM 1069 , 1980, Archives of Microbiology.

[21]  K. Haider,et al.  Decomposition of 14C-labelled lignin and phenols by a Nocardia sp. , 1977, Archives of Microbiology.

[22]  A. Ragauskas,et al.  Oxygen Delignification Chemistry and Its Impact on Pulp Fibers , 2003 .

[23]  L. Lucia,et al.  Comparative evaluation of oxygen delignification processes for low and high lignin content softwood kraft pulps , 2002 .

[24]  D. Argyropoulos,et al.  The role and fate of lignin's condensed structures during oxygen delignification , 2000 .

[25]  A. Ragauskas,et al.  Oxygen Delignification of High-Yield Kraft Pulp. Part I: Structural Properties of Residual Lignins , 1999 .

[26]  L. Heuts,et al.  STRUCTURAL CHANGES IN LIGNIN DURING A TOTALLY CHLORINE FREE BLEACHING SEQUENCE. PART II : AN NMR STUDY , 1999 .

[27]  M. Chartrain,et al.  Bioconversion of indene to cis (1S,2R) indandiol and trans (1R,2R) indandiol by Rhodococcus species , 1998 .

[28]  J. Gierer.  Formation and Involvement of Superoxide (O2-/HO2·) and Hydroxyl (OH·) Radicals in TCF Bleaching Processes: A Review , 1997 .