The effect of extremely low frequency electromagnetic fields (ELF-EMF) on the frequency of micronuclei and sister chromatid exchange in human lymphocytes induced by benzo(a)pyrene.

The interaction of extremely low frequency electromagnetic fields (ELF-EMF) on the frequency of micronuclei (MN) and sister chromatid exchange (SCE) induced by benzo(a)pyrene (BP) in human lymphocytes was examined. A 60 Hz ELF-EMF of 0.8 mT field strength was applied either alone or with the tumor initiator, BP for 24 h. The frequencies of MN and SCE induced by BP increased in a dose-dependent manner. The co-exposure of cells to BP and 0.8 mT ELF-EMF for 24 h, followed by BP exposure for 48 h led to significant increases in the frequencies of MN and SCE compared to BP treatment for 72 h alone (P<0.05), but no significant difference was observed between field exposed and sham exposed control cells. The obtained results suggest that low density ELF-EMF could act as an enhancer of the initiation process of BP rather than as an initiator of mutagenic effects in human lymphocytes.

[1]  D. Weiss,et al.  Micronucleus induction in Syrian hamster embryo cells following exposure to 50 Hz magnetic fields, benzo(a)pyrene, and TPA in vitro. , 2001, Mutation research.

[2]  T. Rytömaa,et al.  Possible cocarcinogenic effects of ELF electromagnetic fields may require repeated long-term interaction with known carcinogenic factors. , 2000, Bioelectromagnetics.

[3]  M. Fenech.  The in vitro micronucleus technique. , 2000, Mutation research.

[4]  Howard Frumkin,et al.  Residential proximity to electricity transmission and distribution equipment and risk of childhood leukemia, childhood lymphoma, and childhood nervous system tumors: systematic review, evaluation, and meta-analysis , 1994, Cancer Causes & Control.

[5]  J. McCann,et al.  The genotoxic potential of electric and magnetic fields: an update. , 1998, Mutation research.

[6]  H. Wachtel,et al.  Case-control study of childhood cancer and exposure to 60-Hz magnetic fields. , 1988, American journal of epidemiology.

[7]  D. Lecuyer,et al.  A 60-Hz magnetic field increases the incidence of squamous cell carcinomas in mice previously exposed to chemical carcinogens. , 1995, Cancer letters.

[8]  W Qassem,et al.  Cytogenetic effects of pulsing electromagnetic field on human lymphocytes in vitro: chromosome aberrations, sister-chromatid exchanges and cell kinetics. , 1991, Mutation research.

[9]  W. R. Adey,et al.  60 Hz magnetic field acts as co-promoter in focus formation of C3H/10T1/2 cells. , 1993, Carcinogenesis.

[10]  D A Savitz,et al.  Magnetic field exposure in relation to leukemia and brain cancer mortality among electric utility workers. , 1995, American journal of epidemiology.

[11]  N. Wertheimer,et al.  Electrical wiring configurations and childhood cancer. , 1979, American journal of epidemiology.

[12]  W. Löscher,et al.  Animal studies on the role of 50/60-Hertz magnetic fields in carcinogenesis. , 1994, Life sciences.

[13]  W. Löscher,et al.  Linear relationship between flux density and tumor co-promoting effect of prolonged magnetic field exposure in a breast cancer model. , 1995, Cancer letters.

[14]  W. Löscher,et al.  A histopathological study on alterations in DMBA-induced mammary carcinogenesis in rats with 50 Hz, 100 muT magnetic field exposure. , 1995, Carcinogenesis.

[15]  W. Baer-Dubowska,et al.  Effect of the route of benzo[a]pyrene administration on sister chromatid exchange and DNA binding in bone marrow of mice differing with respect to cytochrome P450 1A1 induction. , 1997, Toxicology letters.

[16]  M. Simkó,et al.  Absence of synergistic effects on micronucleus formation after exposure to electromagnetic fields and asbestos fibers in vitro. , 1999, Toxicology letters.

[17]  W. Au,et al.  Variant metabolizing gene alleles determine the genotoxicity of benzo[a]pyrene , 2001, Environmental and molecular mutagenesis.

[18]  S. Wolff,et al.  New Giemsa method for the differential staining of sister chromatids , 1974, Nature.

[19]  C. Franceschi,et al.  50 Hz AC sinusoidal electric fields do not exert genotoxic effects (micronucleus formation) in human lymphocytes. , 1993, Radiation research.

[20]  J. Walleczek,et al.  Increase in radiation-induced HPRT gene mutation frequency after nonthermal exposure to nonionizing 60 Hz electromagnetic fields. , 1999, Radiation research.

[21]  G. Obe,et al.  Cytological effects of 50 Hz electromagnetic fields on human lymphocytes in vitro. , 1995, Mutation research.

[22]  Leeka Kheifets,et al.  Occupational electric and magnetic field exposure and brain cancer: a meta-analysis. , 1995 .

[23]  L. Lamberti,et al.  Cell kinetics and sister-chromatid-exchange frequency in human lymphocytes. , 1983, Mutation research.

[24]  I. Lagroye,et al.  The effect of 50 Hz electromagnetic fields on the formation of micronuclei in rodent cell lines exposed to gamma radiation. , 1997, International journal of radiation biology.

[25]  A. Miller,et al.  Leukemia following occupational exposure to 60-Hz electric and magnetic fields among Ontario electric utility workers. , 1996, American journal of epidemiology.

[26]  V. Kosma,et al.  Effects of 50 Hz magnetic fields on UV-induced skin tumourigenesis in ODC-transgenic and non-transgenic mice. , 1998, International journal of radiation biology.