Screening and subcloning of high producer transfectomas using semisolid media and automated colony picker.
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[1] R. Pérez,et al. Humanization of predicted T-cell epitopes reduces the immunogenicity of chimeric antibodies: new evidence supporting a simple method. , 2003, Hybridoma and hybridomics.
[2] Noelle-Ann Sunstrom,et al. High-throughput clonal selection of recombinant CHO cells using a dominant selectable and amplifiable metallothionein-GFP fusion protein. , 2002, Biotechnology and bioengineering.
[3] R Staszewski,et al. Murphy's law of limiting dilution cloning. , 1990, Statistics in medicine.
[4] Mohamed Al-Rubeai,et al. Selection methods for high-producing mammalian cell lines. , 2007, Trends in biotechnology.
[5] H. Chong,et al. Rapid automated selection of mammalian cell line secreting high level of humanized monoclonal antibody using Clone Pix FL system and the correlation between exterior median intensity and antibody productivity , 2011 .
[6] A. Dickson,et al. Mammalian cell factories for efficient and stable protein expression. , 2006, Current opinion in biotechnology.
[7] A. Garnier,et al. Fluorescent labeling in semi-solid medium for selection of mammalian cells secreting high-levels of recombinant proteins , 2009, BMC biotechnology.
[8] M. Al‐Rubeai,et al. ACSD labelling and magnetic cell separation: a rapid method of separating antibody secreting cells from non-secreting cells. , 2005, Journal of immunological methods.
[9] G. Lee,et al. Key Determinants in the Occurrence of Clonal Variation in Humanized Antibody Expression of CHO Cells during Dihydrofolate Reductase Mediated Gene Amplification , 2001, Biotechnology progress.
[10] P. Underwood,et al. Hazards of the limiting-dilution method of cloning hybridomas. , 1988, Journal of immunological methods.
[11] J. Coloma,et al. Removal of amphipathic epitopes from genetically engineered antibodies: production of modified immunoglobulins with reduced immunogenicity. , 2000, Hybridoma.