Integrated analysis of microRNA and mRNA expression profiles in rats with selenium deficiency and identification of associated miRNA-mRNA network

Selenium deficiency is closely related with various type of cardiovascular disease. However, the miRNA-mRNA regulatory network in Selenium deficiency related cardiac change remains to be understand. In the present study, a reliable Selenium deficiency rat model was established and confirmed by pathological and biochemical examination. The mRNA and miRNA expression profiles were conducted by microarray technology. Gene Ontology (GO) Analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) Pathway Analysis was performed to investigate the function of targeted genes, and the relationship between miRNA and mRNA was studied by network analysis. A total of 4931 mRNAs and 119 miRNAs was differentially expressed between any two groups (control group, low-selenium group and selenium supplementation group). GO and KEGG pathway analysis of selected miRNAs target genes found that selenium deficiency was related to several different biological processes. Furthermore, a miRNA-mRNA regulatory network was conducted to illustrate the interaction of miRNAs and these targeted genes. In conclusion, our present study provides a new insight that potential molecular mechanism of Selenium deficiency was a multiply miRNAs and mRNA caused biological change.

[1]  J. Huttunen.  Selenium and cardiovascular disease. , 2009, Acta pharmacologica et toxicologica.

[2]  M. Rayman,et al.  The importance of selenium to human health , 2000, The Lancet.

[3]  Lianyu Chen,et al.  Identifying microRNA-mRNA regulatory network in gemcitabine-resistant cells derived from human pancreatic cancer cells , 2015, Tumor Biology.

[4]  M. Goumans,et al.  MicroRNA-155 prevents necrotic cell death in human cardiomyocyte progenitor cells via targeting RIP1 , 2010, Journal of cellular and molecular medicine.

[5]  M. Rayman.  Selenoproteins and human health: insights from epidemiological data. , 2009, Biochimica et biophysica acta.

[6]  Cheat Sheet.  Selenium , 2018, Reactions Weekly.

[7]  J. Hesketh,et al.  Selenium in human health and disease. , 2011, Antioxidants & redox signaling.

[8]  Xiujun Li,et al.  The effect of selenium supplementation on coronary heart disease: A systematic review and meta-analysis of randomized controlled trials. , 2017, Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements.

[9]  R. Brigelius-Flohé,et al.  Selenium in the redox regulation of the Nrf2 and the Wnt pathway. , 2013, Methods in enzymology.

[10]  X F Zhang,et al.  Correlation between Oxidative Stress and L-type Calcium Channel Expression in the Ventricular Myocardia of Selenium-deficient Mice , 2012, The Journal of international medical research.

[11]  R. Brigelius-Flohé,et al.  Nrf2 target genes are induced under marginal selenium-deficiency , 2010, Genes & Nutrition.

[12]  Woncheol Jang,et al.  How accurately can we control the FDR in analyzing microarray data? , 2006, Bioinform..

[13]  A. Perkins,et al.  Effect of dietary selenium on the progression of heart failure in the ageing spontaneously hypertensive rat. , 2010, Molecular nutrition & food research.

[14]  C. Li,et al.  Keshan disease: an endemic cardiomyopathy in China. , 1985, Human pathology.

[15]  K. Ge,et al.  Keshan disease-an endemic cardiomyopathy in China , 2004, Virchows Archiv A.

[16]  B. Long,et al.  miR-874 regulates myocardial necrosis by targeting caspase-8 , 2013, Cell Death and Disease.

[17]  X. Niu,et al.  MicroRNA expression profiles in rats with selenium deficiency and the possible role of the Wnt/β-catenin signaling pathway in cardiac dysfunction. , 2015, International journal of molecular medicine.

[18]  E. Rashed,et al.  Anti-inflammatory effect of selenium nanoparticles on the inflammation induced in irradiated rats. , 2017, Canadian journal of physiology and pharmacology.

[19]  V. Ambros.  The functions of animal microRNAs , 2004, Nature.

[20]  Tianshu Yang,et al.  miR-200a-5p regulates myocardial necroptosis induced by Se deficiency via targeting RNF11 , 2017, Redox biology.

[21]  Jinfeng Zou,et al.  Identification and Construction of Combinatory Cancer Hallmark-Based Gene Signature Sets to Predict Recurrence and Chemotherapy Benefit in Stage II Colorectal Cancer. , 2016, JAMA oncology.

[22]  Philip M. Long,et al.  Optimal gene expression analysis by microarrays. , 2002, Cancer cell.

[23]  J. Loscalzo.  Keshan disease, selenium deficiency, and the selenoproteome. , 2014, The New England journal of medicine.

[24]  Teresa Colombo,et al.  TP53 regulates miRNA association with AGO2 to remodel the miRNA–mRNA interaction network , 2016, Genome research.

[25]  Edwin Wang,et al.  Network Analysis Reveals A Signaling Regulatory Loop in the PIK3CA-mutated Breast Cancer Predicting Survival Outcome , 2017, Genom. Proteom. Bioinform..

[26]  I. Harris,et al.  A Systematic Review and Meta-Analysis of Randomized Controlled Trials , 2010 .

[27]  J. Joseph.  Selenium and Cardiometabolic Health: Inconclusive Yet Intriguing Evidence , 2013, The American journal of the medical sciences.

[28]  E. Wang,et al.  Predictive genomics: a cancer hallmark network framework for predicting tumor clinical phenotypes using genome sequencing data. , 2014, Seminars in cancer biology.

[29]  C. Burge,et al.  Conserved Seed Pairing, Often Flanked by Adenosines, Indicates that Thousands of Human Genes are MicroRNA Targets , 2005, Cell.

[30]  Seda Ocaklı,et al.  Protective Effects of Melatonin and Selenium against Apoptosis of Olfactory Sensory Neurons: A Rat Model Study , 2016, American journal of rhinology & allergy.

[31]  Jun-shi Chen.  An original discovery: selenium deficiency and Keshan disease (an endemic heart disease). , 2012, Asia Pacific journal of clinical nutrition.

[32]  Gaofeng Bi,et al.  Identification of miRNA-mRNA Network Associated with Acute Myeloid Leukemia Survival , 2017, Medical science monitor : international medical journal of experimental and clinical research.

[33]  S. Cohen,et al.  microRNA functions. , 2007, Annual review of cell and developmental biology.

[34]  Shiwen Xu,et al.  Selenoprotein W was Correlated with the Protective Effect of Selenium on Chicken Myocardial Cells from Oxidative Damage , 2015, Biological Trace Element Research.

[35]  Paola Sebastiani,et al.  Cluster analysis of gene expression dynamics , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[36]  E. Taskin,et al.  Selenium-mediated cardioprotection against adriamycin-induced mitochondrial damage , 2011, Drug and chemical toxicology.

[37]  M. Floch,et al.  Advances in Nutrition , 2020 .