Lectin immunohistochemical evaluation of human bladder carcinomas. A comparison of Carnoy's and formalin fixation.

A lectin immunohistochemical analysis of 51 human bladder carcinomas, including 44 cases of transitional cell carcinoma (TCC) (G1, 15 cases; G2, 17 cases; G3, 12 cases) and 7 cases of squamous cell carcinoma (SCC), was performed. Tissues were obtained by cold punch biopsies, fixed in Carnoy's or 10% formalin solution, stained for binding of 10 different lectins, and evaluated under the light microscope. The lectins used were concanavalin agglutinin (Con A), soybean agglutinin (SBA), Lotus tetragonolobus agglutinin (LTA), Dolichos biflorusa agglutinin (DBA), peanut agglutinin (PNA), Ricinus communis agglutinin I (RCA1), Ulex europaeus agglutinin I, II (UEA-I, II), wheat germ agglutinin (WGA), and Pisum sativum agglutinin (PEA). TCC prepared with Carnoy's fixation tended to show moderately positive Con A, UEA-I, and WGA reactions for G1, and strongly positive reactions for G2 and G3 lesions. UEA-II was mainly negative in G1, but tended to increase to become moderate in G3. DBA tended to show a moderately positive reaction in G1 and G2, but was mainly negative in G3. With formalin fixation, only RCA1 demonstrated grade specific variation, tendency to react moderately in the G1 and G2 cases, and strongly in G3. There were no further differences among the histopathological grades of TCC for other lectins. Thus, Carnoy's fixation appears superior for distinguishing between grades of lesions. SCC tended to react more strongly than TCC with all the various lectins except PEA, independent of fixation.

[1]  P. Schlag,et al.  Lectins and immunohistochemistry of colorectal cancer, its recurrences and metastases. , 1990, European journal of surgical oncology : the journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology.

[2]  N. Langkilde,et al.  Binding of wheat and peanut lectins to human transitional cell carcinomas. Correlation with histopathologic grade, invasion, and dna ploidy , 1989, Cancer.

[3]  T. Loy,et al.  Binding of soybean agglutinin lectin to prostatic hyperplasia and adenocarcinoma , 1989, Cancer.

[4]  N. Langkilde,et al.  Immunohistochemistry and cytochemistry of experimental rat bladder cancer: binding of the lectins PNA and WGA and of a Le(Y) mouse monoclonal antibody. , 1989, The Journal of urology.

[5]  P. Abel,et al.  Change in glycoconjugate for the binding site of the lectin Ulex europeus 1 following malignant transformation of prostatic epithelium. , 1989, British journal of urology.

[6]  T. Ørntoft,et al.  Lectinohistochemistry of human bladder cancer: Loss of lectin binding structures in invasive carcinomas , 1989, APMIS : acta pathologica, microbiologica, et immunologica Scandinavica.

[7]  R. Walker.  The use of lectins in histopathology , 1985, Pathology, research and practice.

[8]  H. Kosmehl,et al.  Lectin histochemistry of human testicular germ cell tumors. , 1989, Neoplasma.

[9]  S. Yagi.  [Blood group isoantigen and lectin binding studies in the human bladder cancer]. , 1989, Nihon Hinyokika Gakkai zasshi. The japanese journal of urology.

[10]  M. Fukuda,et al.  Lectin Histochemistry of rat thyroid tumors , 1988 .

[11]  N. Sato,et al.  [Lectin-binding sugar chain in bladder tumors]. , 1988, Hinyokika kiyo. Acta urologica Japonica.

[12]  D. Neal,et al.  Histochemical study of lectin binding in neoplastic and non-neoplastic urothelium. , 1987, British journal of urology.

[13]  T. Tsujimura,et al.  Calcifying epithelioma of Malherbe with ossification. Special reference to lectin binding and immunohistochemistry of ossified sites , 1987, Journal of cutaneous pathology.

[14]  T. Kakizoe,et al.  Correlation of results of agglutination assays with concanavalin A and carcinogenesis experiments on promoters of bladder cancer. , 1985, Japanese journal of cancer research : Gann.

[15]  H. Akaza,et al.  [Experimental intravesical treatment of bladder tumor with Concanavalin-A conjugated bleomycin]. , 1984, Nihon Hinyokika Gakkai zasshi. The japanese journal of urology.

[16]  H. Cooper,et al.  Peanut lectin binding sites in transitional cell carcinoma of the urinary bladder , 1984, Cancer.

[17]  J. Lafitte,et al.  Use of lectins for detection of glycoconjugates in the glandular cells of the human bronchial mucosa. , 1982, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[18]  T. Kakizoe,et al.  Maintenance by saccharin of membrane alterations of rat bladder cells induced by subcarcinogenic treatment with bladder carcinogens. , 1981, Cancer research.

[19]  T. Kakizoe,et al.  Increased agglutinability of bladder cells by concanavalin A after administration of carcinogens. , 1980, Cancer research.

[20]  I. Goldstein,et al.  The lectins: carbohydrate-binding proteins of plants and animals. , 1978, Advances in carbohydrate chemistry and biochemistry.