Investigation of the Presence of Inorganic Micro- and Nanosized Contaminants in Bread and Biscuits by Environmental Scanning Electron Microscopy

A European project called “Nanopathology” allowed to develop a new diagnostic tool through which the presence of inorganic particulate matter in pathological human tissues of the digestive tract could be shown. This unexpected evidence induced the authors to put forward the hypothesis that that sort of contamination was present in ingested food. In order to demonstrate this hypothesis, 86 samples of wheat bread and 49 of wheat biscuits from 14 different countries were analyzed by means of an Environmental Scanning Electron Microscopy to detect inorganic, micro-, and nano-scaled contaminants. The X-ray microprobe of an Energy Dispersion Spectroscope was employed to identify their chemical composition. The results indicate that 40% of the samples analyzed contained foreign bodies as ceramic and metallic debris of probable environmental or industrial origin. Because of the great variety of chemical composition of the particulate matter, those contaminants were listed according to the most expressed element. The majority of these debris are not biodegradable, some are chemically toxic, and none of them have any nutritive value. The work discusses the possible origin of such a pollution and the role that it can play on human life.

[1]  M F Hoylaerts,et al.  Passage of intratracheally instilled ultrafine particles from the lung into the systemic circulation in hamster. , 2001, American journal of respiratory and critical care medicine.

[2]  J. Charbonneau Recent case histories of food product-metal container interactions using scanning electron microscopy-x-ray microanalysis. , 2006, Scanning.

[3]  P. Szefer,et al.  Distribution and relationships of mercury, lead, cadmium, copper and zinc in perch (Perca fluviatilis) from the Pomeranian Bay and Szczecin Lagoon, southern Baltic , 2003 .

[4]  J. Finkelstein,et al.  Translocation of Inhaled Ultrafine Manganese Oxide Particles to the Central Nervous System , 2006, Environmental health perspectives.

[5]  Mark R Wiesner,et al.  Comparison of the abilities of ambient and manufactured nanoparticles to induce cellular toxicity according to an oxidative stress paradigm. , 2006, Nano letters.

[6]  S. Montanari,et al.  Detection of micro- and nano-sized biocompatible particles in the blood , 2004, Journal of materials science. Materials in medicine.

[7]  V. Colvin The potential environmental impact of engineered nanomaterials , 2003, Nature Biotechnology.

[8]  W. MacNee,et al.  Ultrafine particles , 2001, Occupational and environmental medicine.

[9]  Melvin E Andersen,et al.  Dermal absorption and penetration of jet fuel components in humans. , 2006, Toxicology letters.

[10]  A S G Curtis,et al.  In vitro reaction of endothelial cells to polymer demixed nanotopography. , 2002, Biomaterials.

[11]  R. Rapanà,et al.  Liver and kidney foreign bodies granulomatosis in a patient with malocclusion, bruxism, and worn dental prostheses. , 2001, Gastroenterology.

[12]  Debra L Laskin,et al.  Smaller is not always better: nanotechnology yields nanotoxicology. , 2005, American journal of physiology. Lung cellular and molecular physiology.

[13]  F. Iskander,et al.  Mineral and trace element contents in bread , 1992 .

[14]  R M Albrecht,et al.  Gastrointestinal persorption and tissue distribution of differently sized colloidal gold nanoparticles. , 2001, Journal of pharmaceutical sciences.

[15]  Zhaohui Zhao,et al.  Oral exposure to cadmium chloride triggers an acute inflammatory response in the intestines of mice, initiated by the over-expression of tissue macrophage inflammatory protein-2 mRNA. , 2006, Toxicology letters.

[16]  J. Heyder,et al.  Instillation of Six Different Ultrafine Carbon Particles Indicates a Surface Area Threshold Dose for Acute Lung Inflammation in Mice , 2005, Environmental health perspectives.

[17]  W. Burch,et al.  Passage of inhaled particles into the blood circulation in humans. , 2002, Circulation.

[18]  C. Kirkpatrick,et al.  Paradoxical effects of hypoxia-mimicking divalent cobalt ions in human endothelial cells in vitro , 2005, Molecular and Cellular Biochemistry.

[19]  Feng Zhao,et al.  Acute toxicological effects of copper nanoparticles in vivo. , 2006, Toxicology letters.

[20]  R. Service,et al.  Nanotechnology Grows Up , 2004, Science.

[21]  Meng Wang,et al.  Acute toxicity of nano- and micro-scale zinc powder in healthy adult mice. , 2006, Toxicology letters.

[22]  Antonietta M Gatti,et al.  Biocompatibility of micro- and nanoparticles. Part I: in liver and kidney. , 2002, Biomaterials.

[23]  A. Tsatsakis,et al.  Lead toxicity update. A brief review. , 2005, Medical science monitor : international medical journal of experimental and clinical research.

[24]  P. Borm,et al.  Vascular effects of ambient particulate matter instillation in spontaneous hypertensive rats. , 2004, Toxicology and applied pharmacology.

[25]  S. Montanari,et al.  In-vivo short- and long-term evaluation of the interaction material-blood , 2005, Journal of materials science. Materials in medicine.

[26]  D. Bagchi,et al.  Effects of acute and chronic oval exposure of lead on blood pressure and bone mineral density in rats. , 2005, Journal of inorganic biochemistry.

[27]  Antonietta M Gatti,et al.  Biocompatibility of micro- and nano-particles in the colon. Part II. , 2004, Biomaterials.