Sequence-Based Typing of flaB Is a More Stable Screening Tool Than Typing of flaA for Monitoring of Campylobacter Populations

ABSTRACT Different typing schemes for Campylobacter spp. were evaluated with 70 outbreak and sporadic isolates. The discriminatory indexes were 0.944 (by pulsed-field gel electrophoresis), 0.920 (by genotyping of the flagellin A gene), 0.902 (by genotyping of flaB), and 0.886 (by multilocus sequence typing). Cross-classification gave 94.77 or 95.82% (PFGE-flaA or PFGE-flaB) concordance. flaA was overdiscriminatory in three cases, most probably due to intragenomic recombination.

[1]  J. Rothgänger,et al.  Typing of Methicillin-Resistant Staphylococcus aureus in a University Hospital Setting by Using Novel Software for spa Repeat Determination and Database Management , 2003, Journal of Clinical Microbiology.

[2]  B. Swaminathan,et al.  Utility of Multilocus Sequence Typing as an Epidemiological Tool for Investigation of Outbreaks of Gastroenteritis Caused by Campylobacter jejuni , 2003, Journal of Clinical Microbiology.

[3]  B. Duim,et al.  Molecular Characterization of Campylobacter jejuni Clones: A Basis for Epidemiologic Investigation , 2002, Emerging infectious diseases.

[4]  Gregor Tanner,et al.  Determining Confidence Intervals When Measuring Genetic Diversity and the Discriminatory Abilities of Typing Methods for Microorganisms , 2001, Journal of Clinical Microbiology.

[5]  B. Swaminathan,et al.  Rapid Pulsed-Field Gel Electrophoresis Protocol for Subtyping of Campylobacter jejuni , 2001, Journal of Clinical Microbiology.

[6]  M. Maiden,et al.  Multilocus Sequence Typing System forCampylobacter jejuni , 2001, Journal of Clinical Microbiology.

[7]  J. Frost,et al.  Family clusters of campylobacter infection. , 2000, Communicable disease and public health.

[8]  B. Barrell,et al.  The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences , 2000, Nature.

[9]  T. Wassenaar,et al.  Genotyping of Campylobacterspp , 2000, Applied and Environmental Microbiology.

[10]  J. Musser,et al.  The IS1167 Insertion Sequence Is a Phylogenetically Informative Marker Among Isolates of Serotype 6B Streptococcus pneumoniae , 1998, Journal of Molecular Evolution.

[11]  P. Fields,et al.  Copyright © 1997, American Society for Microbiology Discrimination of Campylobacter jejuni Isolates by fla Gene Sequencing , 1997 .

[12]  P. Carter,et al.  Evidence for recombination in the flagellin locus of Campylobacter jejuni: implications for the flagellin gene typing scheme , 1997, Journal of clinical microbiology.

[13]  M. Struelens Consensus guidelines for appropriate use and evaluation of microbial epidemiologic typing systems. , 1996, Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases.

[14]  T. Wassenaar,et al.  Variation of the flagellin gene locus of Campylobacter jejuni by recombination and horizontal gene transfer. , 1995, Microbiology.

[15]  R. Alm,et al.  Significance of duplicated flagellin genes in Campylobacter. , 1993, Journal of molecular biology.