Phenotypic and molecular dissection of ICRISAT mini core collection of peanut (Arachis hypogaea L.) for high oleic acid

High oleic acid (O) and low linoleic acid (L) make peanut oil ideal for longer storage and better human health. Among the ICRISAT mini core collection accessions, oleic acid ranged from 33.60% to 73.54%. Accessions belonging to ssp. hypogaea had higher oleic acid (54.16%) compared with those of ssp. fastigiata (45.70%). An O : L ratio up to 6.93 was found among ssp. hypogaea. Additional varieties, mutants, germplasm lines and breeding lines had oleic acid within the range of mini core accessions. Mutations in ahFAD2A, which along with its homologous gene, ahFAD2B code for delta-12-desaturase, resulted in higher oleic acid and O : L ratio. ahFAD2A mutant allele was found in 49.5% of the accessions, and its frequency was higher in ssp. hypogaea (84.52%) than in ssp. fastigiata (19.39%). ahFAD2A mutation had a maximum contribution of 18.82, 12.98 and 10.52 towards the phenotypic variance of O, L and O : L ratio, respectively. Genotypes with high oleic acid levels could not reveal ‘A’ insertion mutation in ahFAD2B. Accessions with high oleic acid could be employed for improving peanut oil quality.

[1]  M. Wang,et al.  FAD2 Gene Mutations Significantly Alter Fatty Acid Profiles in Cultivated Peanuts (Arachis hypogaea) , 2011, Biochemical Genetics.

[2]  R. Varshney,et al.  Identification of quantitative trait loci for protein content, oil content and oil quality for groundnut (Arachis hypogaea L.) , 2011 .

[3]  C. Wang,et al.  Sodium azide mutagenesis resulted in a peanut plant with elevated oleate content , 2011 .

[4]  Víctor Inocencio Pacheco Contreras,et al.  Convenient genotyping of nine bovine K-casein variants , 2011 .

[5]  S. Jogloy,et al.  Genotypic variability and genotype by environment interactions in oil and fatty acids in high, intermediate, and low oleic acid peanut genotypes. , 2010, Journal of agricultural and food chemistry.

[6]  M. Wang,et al.  Development of a real-time PCR genotyping assay to identify high oleic acid peanuts (Arachis hypogaea L.) , 2010, Molecular Breeding.

[7]  M. Wang,et al.  A Simple Allele-Specific PCR Assay for Detecting FAD2 Alleles in Both A and B Genomes of the Cultivated Peanut for High-Oleate Trait Selection , 2010, Plant Molecular Biology Reporter.

[8]  Y. Chu,et al.  Two alleles of ahFAD2B control the high oleic acid trait in cultivated peanut. , 2009 .

[9]  Jerry W. Davis,et al.  Assessment of oil content and fatty acid composition variability in different peanut subspecies and botanical varieties , 2009, Plant Genetic Resources.

[10]  J. Toney,et al.  Oleic acid and peanut oil high in oleic acid reverse the inhibitory effect of insulin production of the inflammatory cytokine TNF-α both in vitro and in vivo systems , 2009, Lipids in Health and Disease.

[11]  Qingli Yang,et al.  Comparison of the Delta(12) fatty acid desaturase gene between high-oleic and normal-oleic peanut genotypes. , 2008, Journal of genetics and genomics = Yi chuan xue bao.

[12]  Y. Chu,et al.  Frequency of a Loss-of-Function Mutation in Oleoyl-PC Desaturase ( ahFAD2A ) in the Mini-Core of the U.S. Peanut Germplasm Collection , 2007 .

[13]  H. Upadhyaya.  Variability for Drought Resistance Related Traits in the Mini Core Collection of Peanut , 2005 .

[14]  C. Holbrook,et al.  Development and evaluation of a mini core collection for the U.S. peanut germplasm collection , 2005 .

[15]  A. Abbott,et al.  High-oleate peanut mutants result from a MITE insertion into the FAD2 gene , 2004, Theoretical and Applied Genetics.

[16]  R. Ortiz,et al.  Development of a groundnut core collection using taxonomical, geographical and morphological descriptors , 2003, Genetic Resources and Crop Evolution.

[17]  R. Ortiz,et al.  Developing a mini core of peanut for utilization of genetic resources , 2002 .

[18]  O. D. Smith,et al.  Expressed variants of Δ12-fatty acid desaturase for the high oleate trait in spanish market-type peanut lines , 2002, Molecular Breeding.

[19]  M. Wang,et al.  A real-time PCR genotyping assay to detect FAD2A SNPs in peanuts (Arachis hypogaea L.) , 2001 .

[20]  A. Abbott,et al.  Endoplasmic oleoyl-PC desaturase references the second double bond. , 2001, Phytochemistry.

[21]  A. Abbott,et al.  The naturally occurring high oleate oil character in some peanut varieties results from reduced oleoyl-PC desaturase activity from mutation of aspartate 150 to asparagine , 2001 .

[22]  A. Abbott,et al.  The high oleate trait in the cultivated peanut [Arachis hypogaea L.]. II. Molecular basis and genetics of the trait , 2000, Molecular and General Genetics MGG.

[23]  F. Teulé,et al.  The high oleate trait in the cultivated peanut [Arachis hypogaea L.]. I. Isolation and characterization of two genes encoding microsomal oleoyl-PC desaturases , 2000, Molecular and General Genetics MGG.

[24]  J. B. Misra,et al.  Near-infrared transmittance spectroscopy: a potential tool for non-destructive determination of oil content in groundnuts , 2000 .

[25]  J. Schell,et al.  Modification of Plant Lipid Synthesis , 1995, Science.

[26]  W. Anderson,et al.  Selection of a core collection from the U.S. germplasm collection of peanut , 1993 .

[27]  J. deMan,et al.  Determination of oil content of seeds by NIR: Influence of fatty acid composition on wavelength selection , 1990 .

[28]  Ashutosh Kumar Singh.  Putative genome donors ofArachis hypogaea (Fabaceae), evidence from crosses with synthetic amphidiploids , 1988, Plant Systematics and Evolution.

[29]  R. Jorgensen,et al.  Ribosomal DNA spacer-length polymorphisms in barley: mendelian inheritance, chromosomal location, and population dynamics. , 1984, Proceedings of the National Academy of Sciences of the United States of America.