Prognostic significance of flow cytometry deoxyribonucleic acid analysis of human oligodendrogliomas.

Flow cytometry was used to determine the deoxyribonucleic acid ploidy and proliferative activity of 60 oligodendrogliomas and oligoastrocytomas. The relationships among survival, ploidy, proliferation, histological features, and clinical variables were analyzed. Survival was strongly associated with the S-phase fraction (P < 0.001). Three groups with significantly different survival rates were defined, based on S-phase fraction ranges of < 3%, 3 to 5.9%, and > 6%. Significant associations between survival and age at diagnosis (P < 0.001), tumor grade (P < 0.001), and extent of surgery (P < 0.01) were found also. The poor correlation between mitotic figures and survival in oligodendrogliomas that has been reported previously emphasizes the need for an accurate method to measure proliferative activity. Our study demonstrated the usefulness of the flow cytometry-determined S-phase fraction in this regard and demonstrated that specific reference ranges could be defined for clinical application. In contrast, the determination of ploidy by flow cytometry was not useful in the evaluation of oligodendrogliomas.

[1]  S. Coons,et al.  Regional heterogeneity in the DNA content of human gliomas , 1993, Cancer.

[2]  S. Coons,et al.  Regional Heterogeneity in the Proliferative Activity of Human Gliomas as Measured by the Ki‐67 Labeling Index , 1993, Journal of neuropathology and experimental neurology.

[3]  C. Daumas-Duport,et al.  Histological grading of gliomas. , 1992, Current opinion in neurology and neurosurgery.

[4]  S. Coons,et al.  Flow cytometric analysis of deoxyribonucleic acid ploidy and proliferation in choroid plexus tumors. , 1992, Neurosurgery.

[5]  C. V. van Eden,et al.  Prognostic relevance of DNA flow cytometry in oligodendroglioma , 1992, Cancer.

[6]  B. Scheithauer,et al.  Oligodendrogliomas: the Mayo Clinic experience. , 1992, Journal of neurosurgery.

[7]  T. Hoshino.  Cell kinetics of glial tumors. , 1992, Revue neurologique.

[8]  A. Friedman,et al.  Influence of the type of surgery on the histologic diagnosis in patients with anaplastic gliomas , 1991, Neurology.

[9]  S. Coons,et al.  Histopathology of astrocytomas: Grading, patterns of spread, and correlation with modern imaging modalities , 1991 .

[10]  G. Mazzini,et al.  Prognostic significance of nuclear DNA content in human neuroepithelial tumors , 1991, International journal of cancer.

[11]  T. H. van der Kwast,et al.  Prognostic implications of glial fibrillary acidic protein containing cell types in oligodendrogliomas , 1990, Cancer.

[12]  H. Thaler,et al.  Immunophenotypic differences between normal glia, astrocytomas and malignant gliomas: correlations with karyotype, natural history and survival , 1989, Journal of Neuroimmunology.

[13]  S. Monte.  Uniform lineage of oligodendrogliomas. , 1989 .

[14]  M. Prados,et al.  Prognostic implications of the bromodeoxyuridine labeling index of human gliomas. , 1989, Journal of neurosurgery.

[15]  S. M. de la Monte,et al.  Dual lineage of astrocytomas. , 1989, The American journal of pathology.

[16]  P. Quirke,et al.  PROGNOSIS IN MALIGNANT GLIOMA: A RETROSPECTIVE STUDY OF BIOPSY SPECIMENS BY FLOW CYTOMETRY , 1989, Neuropathology and Applied Neurobiology.

[17]  P. Kelly,et al.  A cytogenetic study of 53 human gliomas. , 1989, Cancer genetics and cytogenetics.

[18]  P. Kleihues,et al.  Cytologic composition of the untreated glioblastoma with implications for evaluation of needle biopsies , 1989, Cancer.

[19]  K. Sasaki,et al.  Flow-cytometric DNA analysis and immunohistochemical measurement of Ki-67 and BUdR labeling indices in human brain tumors. , 1989, Journal of neurosurgery.

[20]  R. Davis,et al.  Prognostic implications of the proliferative potential of low-grade astrocytomas. , 1988, Journal of neurosurgery.

[21]  P. Kelly,et al.  Grading of astrocytomas: A simple and reproducible method , 1988, Cancer.

[22]  S. Barnwell,et al.  Flow cytometric determination of modal DNA population in relation to proliferative potential of human intracranial neoplasms. , 1988, Journal of neurosurgery.

[23]  S. Coons,et al.  Correlation of DNA content and histology in prognosis of astrocytomas. , 1988, American journal of clinical pathology.

[24]  D. Troost,et al.  Oligodendroglioma. A comparison of two grading systems , 1988, Cancer.

[25]  M. Hamou,et al.  Identification of proliferating cells in human gliomas using the monoclonal antibody Ki-67. , 1988, Neurosurgery.

[26]  D. Bigner,et al.  Specific chromosomal abnormalities in malignant human gliomas. , 1988, Cancer research.

[27]  J. Bruner.  Oligodendroglioma: diagnosis and prognosis. , 1987, Seminars in diagnostic pathology.

[28]  A. Zimmermann,et al.  Flow-cytophotometry of nuclear DNA in biopsies of 45 human gliomas and after primary culture in vitro. , 1986, Clinical Neuropathology.

[29]  G. Eide,et al.  Oligodendroglioma. Histologic Evaluation and Prognosis , 1986, Journal of neuropathology and experimental neurology.

[30]  S. Green,et al.  Glioblastoma multiforme and anaplastic astrocytoma pathologic criteria and prognostic implications , 1985, Cancer.

[31]  I. Taylor,et al.  Application of DNA flow cytometry to paraffin-embedded archival material for the study of aneuploidy and its clinical significance. , 1985, Cytometry.

[32]  V. Armbrustmacher,et al.  Grading of oligodendrogliomas , 1983, Cancer.

[33]  D. Bigner.  Biology of gliomas: potential clinical implications of glioma cellular heterogeneity. , 1981, Neurosurgery.

[34]  N. Ringertz.  Grading of gliomas. , 2009 .

[35]  F. Earnest,et al.  OLIGODENDROGLIOMAS: A Review of Two Hundred Cases , 1950 .