Characterization of a murine ovarian reticulum cell sarcoma of histiocytic origin.

We have studied the M5076 tumor, a transplantable murine reticulum cell sarcoma that arose spontaneously in the ovary of a C57BL/6 mouse. This tumor displays functional and ultrastructural characteristics indicating that it is of macrophage origin. Cells from the M5076 tumor are phagocytic, form rosettes with sheep red blood cells, mediate antibody-dependent cellular cytotoxicity against 51Cr-labeled red blood cells, and display macrophage-like cytotoxicity against syngeneic tumor target cells but do not exhibit any natural killer cell activity. The tumor cells possess lysozyme, nonspecific esterase, and phosphatase activities comparable to that seen in rodent macrophages. Ultrastructural examination revealed phagocytic vacuoles and a lack of tight junctions typical of macrophage morphology. Karyotype analysis showed that M5076 tumor cells are hypodiploid with a high percentage (greater than 80%) of metacentric chromosomes that serve as an excellent marker for identification of these tumor cells.

[1]  I. Fidler,et al.  Metastatic behavior of a murine reticulum cell sarcoma exhibiting organ-specific growth. , 1981, Cancer research.

[2]  J. Larrick,et al.  Characterization of a human macrophage-like cell line stimulated in vitro: a model of macrophage functions. , 1980, Journal of immunology.

[3]  T. Log,et al.  Natural killer cell activity associated with reticulum cell neoplasms , 1980, International journal of cancer.

[4]  J. Lechner,et al.  Chromosomal analysis of human prostatic adenocarcinoma cell lines. , 1980, Cancer research.

[5]  I. Fidler,et al.  Tumor cytotoxicity of rat alveolar macrophages activated in vitro by endotoxin. , 1980, Journal of the Reticuloendothelial Society.

[6]  G. Pinkus,et al.  Non‐hodgkin's lymphomas: An ultrastructural study correlating morphology with immunologic cell type , 1979, Cancer.

[7]  N. Ponzio,et al.  Natural killer cell activity in reticulum cell sarcomas (RCS) of SJL/J mice. , 1979, Cellular immunology.

[8]  H. Kaplan,et al.  Biology and virology of the human malignant lymphomas. 1st Milford D. Schulz lecture , 1979, Cancer.

[9]  P. Ralph,et al.  Functional macrophage cell lines transformed by abelson leukemia virus , 1978, Cell.

[10]  D. Miller,et al.  Nucleolus organizer activity and the origin of Robertsonian translocations. , 1978, Cytogenetics and cell genetics.

[11]  A. Epstein,et al.  Biology of the human malignant lymphomas. , 1978, Recent results in cancer research. Fortschritte der Krebsforschung. Progres dans les recherches sur le cancer.

[12]  P. Ralph,et al.  Lysozyme synthesis by established human and murine histiocytic lymphoma cell lines , 1976, The Journal of experimental medicine.

[13]  K. Nilsson,et al.  Establishment and characterization of a human histiocytic lymphoma cell line (U‐937) , 1976, International journal of cancer.

[14]  G. Flandrin,et al.  Membrane markers in "histiocytic" lymphomas (reticulum cell sarcomas). , 1976, Journal of the National Cancer Institute.

[15]  P. Ralph,et al.  Phagocytosis and cytolysis by a macrophage tumour and its cloned cell line , 1975, Nature.

[16]  H. Ovadia,et al.  Effect of Normal Immunosuppressive Protein (NIP) on tumor growth in mice. , 1975, European journal of cancer.

[17]  R. Lukes,et al.  New approaches to the classification of the lymphomata. , 1975, The British journal of cancer. Supplement.

[18]  K. Lennert,et al.  Cytological and functional criteria for the classification of malignant lymphomata. , 1975, The British journal of cancer. Supplement.

[19]  H. Koren,et al.  Identification of macrophage-like characteristics in a cultured murine tumor line. , 1975, Journal of immunology.

[20]  M. Cohn,et al.  Reticulum cell sarcoma: an effector cell in antibody-dependent cell-mediated immunity. , 1975, Journal of immunology.

[21]  Z. Cohn,et al.  IN VITRO SYNTHESIS AND SECRETION OF LYSOZYME BY MONONUCLEAR PHAGOCYTES , 1974, The Journal of experimental medicine.

[22]  R. C. Parks.  Brief Communication: Organ-Specific Metastasis of a Transplantable Reticulum Cell Sarcoma , 1974 .

[23]  R. C. Parks.  Organ-specific metastasis of a transplantable reticulum cell sarcoma. , 1974, Journal of the National Cancer Institute.

[24]  A. T. Natarajan,et al.  Distribution of constitutive heterochromatin in the chromosomes of MSWBS ascites tumor cells. , 1973, Journal of the National Cancer Institute.

[25]  I. Fidler,et al.  Selection of successive tumour lines for metastasis. , 1973, Nature: New biology.

[26]  A. Levan,et al.  The stemline idiogram of the MSWBS tumor of the mouse and the problem of centric fusion. , 2009, Hereditas.

[27]  M. Farquhar,et al.  DIFFERENTIATION OF MONOCYTES , 1971, The Journal of cell biology.

[28]  W. Crosby,et al.  Cytochemical identification of monocytes and granulocytes. , 1971, American journal of clinical pathology.

[29]  M. Friedman,et al.  Reticulum-Cell Sarcoma , 1970 .

[30]  H. I. Pilgrim.  The kinetics of the organ-specific metastasis of a transplantable reticuloendothelial tumor. , 1969, Cancer research.

[31]  M. MacAulay,et al.  Ultrastructure of a biosynthetically active granulosa cell tumor. , 1967, Laboratory investigation; a journal of technical methods and pathology.

[32]  H. Rappaport,et al.  Tumors of the hematopoietic system , 1966 .

[33]  E. Gall.  THE CYTOLOGICAL IDENTITY AND INTERRELATION OF MESENCHYMAL CELLS OF LYMPHOID TISSUE , 1958, Annals of the New York Academy of Sciences.

[34]  T. Dunn.  Normal and pathologic anatomy of the reticular tissue in laboratory mice, with a classification and discussion of neoplasms. , 1954, Journal of the National Cancer Institute.

[35]  A. M. Cloudman.  Organophilic tendencies of two transplantable tumors of the mouse. , 1947 .