Biological sealing and integration of a fibrinogen-modified titanium alloy with soft and hard tissues in a rat model.

Percutaneous or transcutaneous devices are important and unique, and the corresponding biological sealing at the skin-implant interface is the key to their long-term success. Herein, we investigated the surface modification to enhance biological sealing, using a metal sheet and screw bonded by biomacromolecule fibrinogen mediated via pre-deposited synthetic macromolecule polydopamine (PDA) as a demonstration. We examined the effects of a Ti-6Al-4V titanium alloy modified with fibrinogen (Ti-Fg), PDA (Ti-PDA) or their combination (Ti-PDA-Fg) on the biological sealing and integration with skin and bone tissues. Human epidermal keratinocytes (HaCaT), human foreskin fibroblasts (HFF) and preosteoblasts (MC3T3-E1), which are closely related to percutaneous implants, exhibited better adhesion and spreading on all the three modified sheets compared with the unmodified alloy. After three-week subcutaneous implantation in Sprague-Dawley (SD) rats, the Ti-PDA-Fg sheets could significantly attenuate the soft tissue response and promote angiogenesis compared with other groups. Furthermore, in the model of percutaneous tibial implantation in SD rats, the Ti-PDA-Fg screws dramatically inhibited epithelial downgrowth and promoted new bone formation. Hence, the covalent immobilization of fibrinogen through the precoating of PDA is promising for enhanced biological sealing and osseointegration of metal implants with soft and hard tissues, which is critical for an orthopedic percutaneous medical device.

[1]  Lin Yu,et al.  Injectable Thermogel Generated by the "Block Blend" Strategy as a Biomaterial for Endoscopic Submucosal Dissection. , 2021, ACS applied materials & interfaces.

[2]  Jiandong Ding,et al.  Critical Frequency and Critical Stretching Rate for Reorientation of Cells on a Cyclically Stretched Polymer in a Microfluidic Chip. , 2021, ACS applied materials & interfaces.

[3]  Xi-zheng Zhang,et al.  Characterization and evaluation of a femtosecond laser-induced osseointegration and an anti-inflammatory structure generated on a titanium alloy , 2021, Regenerative biomaterials.

[4]  MyungGu Yeo,et al.  3D-printed gelatin methacrylate (GelMA)/silanated silica scaffold assisted by two-stage cooling system for hard tissue regeneration , 2021, Regenerative biomaterials.

[5]  Jiayue Shi,et al.  Cell‐Free Bilayered Porous Scaffolds for Osteochondral Regeneration Fabricated by Continuous 3D‐Printing Using Nascent Physical Hydrogel as Ink , 2020, Advanced healthcare materials.

[6]  M. Ginebra,et al.  Chemically Diverse Multifunctional Peptide Platforms with Antimicrobial and Cell Adhesive Properties , 2020, Chembiochem : a European journal of chemical biology.

[7]  Deyuan Zhang,et al.  In vivo degradation and endothelialization of an iron bioresorbable scaffold , 2020, Bioactive materials.

[8]  Jiandong Ding,et al.  A simplified yet enhanced and versatile microfluidic platform for cyclic cell stretching on an elastic polymer , 2020, Biofabrication.

[9]  C. Krettek,et al.  Transkutane osseointegrierte Prothesensysteme (TOPS) zur Versorgung Oberschenkelamputierter , 2020, Die Rehabilitation.

[10]  Junhao He,et al.  Cell migration regulated by RGD nanospacing and enhanced under moderate cell adhesion on biomaterials. , 2020, Biomaterials.

[11]  S. Telian,et al.  Multicenter Clinical Investigation of a New Active Osseointegrated Steady-State Implant System , 2020, Otology & neurotology : official publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology.

[12]  R. Leijendekkers,et al.  Safety and Performance of Bone-Anchored Prostheses in Persons with a Transfemoral Amputation: A 5-Year Follow-up Study. , 2020, The Journal of bone and joint surgery. American volume.

[13]  N. Gadegaard,et al.  Nanopatterned Titanium Implants Accelerate Bone Formation In Vivo , 2020, ACS applied materials & interfaces.

[14]  Lan Liao,et al.  Tremella-Like ZnO@Col-I-Decorated Titanium Surfaces with Dual-Light-Defined Broad-Spectrum Antibacterial and Triple Osteogenic Properties. , 2020, ACS applied materials & interfaces.

[15]  Ashley A. Vu,et al.  Thermal Oxide Layer Enhances Crystallinity and Mechanical Properties for Plasma-Sprayed Hydroxyapatite Biomedical Coatings. , 2020, ACS applied materials & interfaces.

[16]  Jiandong Ding,et al.  Effects of microstripe geometry on guided cell migration. , 2020, ACS applied materials & interfaces.

[17]  Jiali Tan,et al.  Osteoimmunomodulatory effects of biomaterial modification strategies on macrophage polarization and bone regeneration , 2020, Regenerative biomaterials.

[18]  Heungsoo Shin,et al.  Surface engineering of titanium alloy using metal-polyphenol network coating with magnesium ions for improved osseointegration. , 2020, Biomaterials science.

[19]  Xing‐dong Zhang,et al.  The optimized preparation of HA/L-TiO2/D-TiO2 composite coating on porous titanium and its effect on the behavior osteoblasts , 2020, Regenerative biomaterials.

[20]  Deyuan Zhang,et al.  Long-term efficacy of biodegradable metal-polymer composite stents after the first and second implantations into porcine coronary arteries. , 2020, ACS applied materials & interfaces.

[21]  Donghui Wang,et al.  A facile and universal strategy to endow implant materials with antibacterial ability via alkalinity disturbing bacterial respiration. , 2020, Biomaterials science.

[22]  N. Huang,et al.  Cu∥-loaded polydopamine coatings with in situ nitric oxide generation function for improved hemocompatibility , 2020, Regenerative biomaterials.

[23]  K. Hagberg,et al.  A 15-year follow-up of transfemoral amputees with bone-anchored transcutaneous prostheses. , 2020, The bone & joint journal.

[24]  J. Ji,et al.  Fabrication of Mixed-Charge Polypeptide Coating for Enhanced Hemocompatibility and Anti-infective Effect. , 2019, ACS applied materials & interfaces.

[25]  David F. Williams,et al.  Specifications for Innovative, Enabling Biomaterials Based on the Principles of Biocompatibility Mechanisms , 2019, Front. Bioeng. Biotechnol..

[26]  Vee San Cheong,et al.  Partial Bone Formation in Additive Manufactured Porous Implants Reduces Predicted Stress and Danger of Fatigue Failure , 2019, Annals of Biomedical Engineering.

[27]  Xing‐dong Zhang,et al.  Application of hydroxyapatite nanoparticles in tumor-associated bone segmental defect , 2019, Science Advances.

[28]  Peng Liu,et al.  Biocompatible MoS2/PDA-RGD coating on titanium implant with antibacterial property via intrinsic ROS-independent oxidative stress and NIR irradiation. , 2019, Biomaterials.

[29]  P. Savelkoul,et al.  Microbiome on the Bone-Anchored Hearing System: A Prospective Study , 2019, Front. Microbiol..

[30]  Xiaowei Yang,et al.  Structural mechanics of 3D-printed poly(lactic acid) scaffolds with tetragonal, hexagonal and wheel-like designs , 2019, Biofabrication.

[31]  K. Cai,et al.  Functionalization of titanium substrate with multifunctional peptide OGP-NAC for the regulation of osteoimmunology. , 2019, Biomaterials science.

[32]  Jiandong Ding,et al.  Polydopamine-mediated covalent functionalization of collagen on a titanium alloy to promote biocompatibility with soft tissues. , 2019, Journal of materials chemistry. B.

[33]  Deyuan Zhang,et al.  Mechanism of Acceleration of Iron Corrosion by a Polylactide Coating. , 2018, ACS applied materials & interfaces.

[34]  Bin Liu,et al.  Peptide LL-37 coating on micro-structured titanium implants to facilitate bone formation in vivo via mesenchymal stem cell recruitment. , 2018, Acta biomaterialia.

[35]  Hao-Cheng Yang,et al.  Dopamine-assisted co-deposition: An emerging and promising strategy for surface modification. , 2018, Advances in colloid and interface science.

[36]  H. Uludaǧ,et al.  A review of nanostructured surfaces and materials for dental implants: surface coating, patterning and functionalization for improved performance. , 2018, Biomaterials science.

[37]  R. Stokroos,et al.  Cytokine expression profile in the bone‐anchored hearing system: 12‐week results from a prospective randomized, controlled study , 2018, Clinical implant dentistry and related research.

[38]  Yumei Zhang,et al.  Bone mesenchymal stem cell secretion of sRANKL/OPG/M-CSF in response to macrophage-mediated inflammatory response influences osteogenesis on nanostructured Ti surfaces. , 2018, Biomaterials.

[39]  L. Bi,et al.  Evaluation of the osteogenesis and osseointegration of titanium alloys coated with graphene: an in vivo study , 2018, Scientific Reports.

[40]  Fei Yang,et al.  The immobilization of antibiotic-loaded polymeric coatings on osteoarticular Ti implants for the prevention of bone infections. , 2017, Biomaterials science.

[41]  J. P. Beck,et al.  A 24-month evaluation of a percutaneous osseointegrated limb-skin interface in an ovine amputation model , 2017, Journal of Materials Science: Materials in Medicine.

[42]  Faleh Tamimi,et al.  Strategies for Optimizing the Soft Tissue Seal around Osseointegrated Implants , 2017, Advanced healthcare materials.

[43]  V. Migonney,et al.  Bone tissue response induced by bioactive polymer functionalized Ti6Al4V surfaces: In vitro and in vivo study. , 2017, Journal of colloid and interface science.

[44]  Ali Khademhosseini,et al.  Mussel-Inspired Multifunctional Hydrogel Coating for Prevention of Infections and Enhanced Osteogenesis. , 2017, ACS applied materials & interfaces.

[45]  Mark Holodniy,et al.  Multifunctional coatings to simultaneously promote osseointegration and prevent infection of orthopaedic implants. , 2016, Biomaterials.

[46]  Chengtie Wu,et al.  The effect of osteoimmunomodulation on the osteogenic effects of cobalt incorporated β-tricalcium phosphate. , 2015, Biomaterials.

[47]  R. Gourdie,et al.  Biomedical Implant Capsule Formation: Lessons Learned and the Road Ahead , 2014, Annals of plastic surgery.

[48]  P. Dubruel,et al.  Gelatin functionalised porous titanium alloy implants for orthopaedic applications. , 2014, Materials science & engineering. C, Materials for biological applications.

[49]  Gorka Orive,et al.  Toward the biomimetic implant surface: Biopolymers on titanium-based implants for bone regeneration , 2014 .

[50]  Jiandong Ding,et al.  Cell–Material Interactions Revealed Via Material Techniques of Surface Patterning , 2013, Advanced materials.

[51]  Amar R. Marathe,et al.  Stereoelectroencephalography for continuous two-dimensional cursor control in a brain-machine interface. , 2013, Neurosurgical focus.

[52]  J. Park,et al.  Engineering biocompatible implant surfaces , 2013 .

[53]  Ping Yang,et al.  The effect of coimmobilizing heparin and fibronectin on titanium on hemocompatibility and endothelialization. , 2011, Biomaterials.

[54]  S. Bellis,et al.  Advantages of RGD peptides for directing cell association with biomaterials. , 2011, Biomaterials.

[55]  Xuesi Chen,et al.  Non-specific and specific interactions on functionalized polymer surface studied by FT-SPR. , 2011, Colloids and surfaces. B, Biointerfaces.

[56]  Paul K. Chu,et al.  Surface nano-functionalization of biomaterials , 2010 .

[57]  C. Elvin,et al.  A pH-responsive interface derived from resilin-mimetic protein Rec1-resilin. , 2010, Biomaterials.

[58]  Robert A Latour,et al.  The relationship between platelet adhesion on surfaces and the structure versus the amount of adsorbed fibrinogen. , 2010, Biomaterials.

[59]  John A. Jansen,et al.  Electrosprayed Enzyme Coatings as Bioinspired Alternatives to Bioceramic Coatings for Orthopedic and Oral Implants , 2009 .

[60]  Wei-Qiang Song,et al.  Immobilization of proteins on metal ion chelated polymer surfaces. , 2009, Colloids and surfaces. B, Biointerfaces.

[61]  Haeshin Lee,et al.  Facile Conjugation of Biomolecules onto Surfaces via Mussel Adhesive Protein Inspired Coatings , 2009, Advanced materials.

[62]  K. S. Jones,et al.  Effects of biomaterial-induced inflammation on fibrosis and rejection. , 2008, Seminars in immunology.

[63]  Haeshin Lee,et al.  Mussel-Inspired Surface Chemistry for Multifunctional Coatings , 2007, Science.

[64]  G. Francius,et al.  AFM force spectroscopy of the fibrinogen adsorption process onto dental implants. , 2006, Journal of biomedical materials research. Part A.

[65]  A. Goodship,et al.  Development of a soft tissue seal around bone-anchored transcutaneous amputation prostheses. , 2006, Biomaterials.

[66]  P. Koolwijk,et al.  Fibrin structure and wound healing , 2006, Journal of thrombosis and haemostasis : JTH.

[67]  P. Simpson‐Haidaris,et al.  Matrix-fibrinogen enhances wound closure by increasing both cell proliferation and migration. , 2003, Blood.

[68]  R. Clark,et al.  Fibrinogen and fibrin are anti-adhesive for keratinocytes: a mechanism for fibrin eschar slough during wound repair. , 2001, The Journal of investigative dermatology.

[69]  Sit Ps,et al.  Surface-dependent conformations of human fibrinogen observed by atomic force microscopy under aqueous conditions. , 1999 .

[70]  R. Clark,et al.  Human Fibroblasts Bind Directly to Fibrinogen at RGD Sites through Integrin αvβ3 , 1997 .

[71]  D. Williams,et al.  Marsupialization of percutaneous implants in presence of deep connective tissue. , 1996, Journal of biomedical materials research.

[72]  J. Jansen,et al.  Tissue reaction to soft-tissue anchored percutaneous implants in rabbits. , 1994, Journal of biomedical materials research.

[73]  D. Brunette,et al.  The role of connective tissue in inhibiting epithelial downgrowth on titanium-coated percutaneous implants. , 1992, Journal of biomedical materials research.

[74]  E Y Chao,et al.  Internal remodeling of periosteal new bone during fracture healing , 1990, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.

[75]  K Affeld,et al.  Design criteria for percutaneous devices. , 1984, Journal of Biomedical Materials Research.

[76]  A. F. Recum,et al.  Applications and failure modes of percutaneous devices: A review , 1984 .

[77]  P. Branemark.  Osseointegration and its experimental background. , 1983, The Journal of prosthetic dentistry.

[78]  Peng Li,et al.  Antibacterial and hydroxyapatite-forming coating for biomedical implants based on polypeptide-functionalized titania nanospikes. , 2019, Biomaterials science.

[79]  X. D. Zhang,et al.  Bio-functionalization of biomedical metals. , 2017, Materials science & engineering. C, Materials for biological applications.

[80]  N. Udagawa,et al.  High-performance scaffolds on titanium surfaces: osteoblast differentiation and mineralization promoted by a globular fibrinogen layer through cell-autonomous BMP signaling. , 2015, Materials science & engineering. C, Materials for biological applications.

[81]  D. Brunette,et al.  Subcutaneous microfabricated surfaces inhibit epithelial recession and promote long-term survival of percutaneous implants. , 2002, Biomaterials.

[82]  P. Branemark,et al.  Titanium implants permanently penetrating human skin. , 1982, Scandinavian journal of plastic and reconstructive surgery.

[83]  G D Winter,et al.  Transcutaneous implants: reactions of the skin-implant interface. , 1974, Journal of biomedical materials research.