New challenges in biophotonics: laser-based fluoroimmuno analysis and in vivo optical oxygen monitoring

Two examples of our biophotonic research utilizing nanoparticles are presented, namely laser-based fluoroimmuno analysis and in-vivo optical oxygen monitoring. Results of the work include significantly enhanced sensitivity of a homogeneous fluorescence immunoassay and markedly improved spatial resolution of oxygen gradients in root nodules of a legume species.

[1]  P. Geigenberger,et al.  Symbiotic Leghemoglobins Are Crucial for Nitrogen Fixation in Legume Root Nodules but Not for General Plant Growth and Development , 2005, Current Biology.

[2]  Hans-Gerd Löhmannsröben,et al.  Quantum dots as efficient energy acceptors in a time-resolved fluoroimmunoassay. , 2005, Angewandte Chemie.

[3]  H. Clark,et al.  Production, characteristics and applications of fluorescent PEBBLE nanosensors : Potassium, oxygen, calcium and pH imaging inside live cells : Biomedical applications , 2002 .

[4]  Ingo Klimant,et al.  Fiber‐optic oxygen microsensors, a new tool in aquatic biology , 1995 .

[5]  Dmitri B Papkovsky,et al.  Methods in optical oxygen sensing: protocols and critical analyses. , 2004, Methods in enzymology.

[6]  Ingo Klimant,et al.  An optical multifrequency phase-modulation method using microbeads for measuring intracellular oxygen concentrations in plants. , 2005, Biophysical journal.

[7]  J. Aylott,et al.  A real-time ratiometric method for the determination of molecular oxygen inside living cells using sol-gel-based spherical optical nanosensors with applications to rat C6 glioma. , 2001, Analytical chemistry.

[8]  Ichiro Okura,et al.  Platinum tetrakis(pentafluorophenyl)porphyrin immobilized in polytrifluoroethylmethacrylate film as a photostable optical oxygen detection material , 2001 .

[9]  Aldo Roda,et al.  Engineering of highly luminescent lanthanide tags suitable for protein labeling and time-resolved luminescence imaging. , 2004, Journal of the American Chemical Society.