Isotopic analyses of nitrogenous compounds from the Murchison meteorite: ammonia, amines, amino acids, and polar hydrocarbons.

The combined volatile bases (ammonia, aliphatic amines, and possibly other bases), ammonia, amino acids, and polar hydrocarbons were prepared from the Murchison meteorite for isotopic analyses. The volatile bases were obtained by cryogenic transfer after acid-hydrolysis of a hot-water extract and analyzed by combined gas chromatography-mass spectrometry of pentafluoropropionyl derivatives. The aliphatic amines present in this preparation comprise a mixture that includes both primary and secondary isomers through C5 at a total concentration of > or = 100 nmoles g-1. As commonly observed for meteoritic organic compounds, almost all isomers through C5 are present, and the concentrations within homologous series decrease with increasing chain length. Ammonia was chromatographically separated from the other volatile bases and found at a concentration of 1.1-1.3 micromoles g-1 meteorite. The ammonia analyzed includes contributions from ammonium salts and the hydrolysis of extractable organic compounds, e.g., carboxamides. Stable isotope analyses showed the volatile bases to be substantially enriched in the heavier isotopes, relative to comparable terrestrial compounds delta D < or = +1221%; delta 13C = +22%; delta 15N = +93%). Ammonia, per se, was found to have a somewhat lower delta 15N value (+69%) than the total volatile bases; consequently, a higher delta 15N (>93%) can be inferred for the other bases, which include the amines. Solvent-extractable polar hydrocarbons obtained separately were found to be enriched in 15N (delta 15N = +104%). Total amino acids, prepared from a hydrolyzed hot-water extract by cation exchange chromatography, gave a delta 15N of +94%, a value in good agreement with that obtained previously. Nitrogen isotopic data are also given for amino acid fractions separated chromatographically. The delta 15N values of the Murchison soluble organic compounds analyzed to date fall within a rather narrow range (delta 15N = +94 +/- 8%), an observation consistent with their formation, or formation of their precursors, by interstellar chemistry.

[1]  G. Yuen,et al.  Aliphatic amines in the Murchison meteorite , 1976, Nature.

[2]  F. Robert,et al.  The concentration and isotopic composition of hydrogen, carbon and nitrogen in carbonaceous meteorites☆ , 1982 .

[3]  Y. Koshelkov.  Proposal for a reference model of the middle atmosphere of the southern hemisphere , 1983 .

[4]  T. E. Bunch,et al.  Carbonaceous chondrites. II - Carbonaceous chondrite phyllosilicates and light element geochemistry as indicators of parent body processes and surface conditions , 1980 .

[5]  J. Kerridge.  Carbon, hydrogen and nitrogen in carbonaceous chondrites: abundances and isotopic compositions in bulk samples. , 1985, Geochimica et cosmochimica acta.

[6]  Y. Kolodny,et al.  Deuterium in carbonaceous chondrites , 1980 .

[7]  R. Clayton,et al.  Nitrogen abundances and isotopic compositions in stony meteorites , 1978 .

[8]  E. Peltzer,et al.  α-Hydroxycarboxylic acids in the Murchison meteorite , 1978, Nature.

[9]  Alan W. Schwartz,et al.  Basic nitrogen-heterocyclic compounds in the Murchison meteorite , 1982 .

[10]  G. P. Shulman,et al.  Thermal decomposition of aliphatic monoaminomonocarboxylic acids , 1972 .

[11]  W. Irvine,et al.  Chemical abundances in cold, dark interstellar clouds. , 1991, Icarus.

[12]  J L Bada,et al.  The chemical conditions on the parent body of the Murchison meteorite: some conclusions based on amino, hydroxy and dicarboxylic acids. , 1984, Advances in space research : the official journal of the Committee on Space Research.

[13]  J. Cronin,et al.  Linear and cyclic aliphatic carboxamides of the Murchison meteorite: hydrolyzable derivatives of amino acids and other carboxylic acids. , 1995, Geochimica et cosmochimica acta.

[14]  C. Pillinger,et al.  Isotopically anomalous nitrogen in primitive meteorites , 1983, Nature.

[15]  N. Kaifu,et al.  Detection of Interstellar Methylamine , 1974 .

[16]  S. Epstein,et al.  Determination of D/H ratios of nonexchangeable hydrogen in cellulose: A method based on the cellulose-water exchange reaction , 1993 .

[17]  S. Epstein,et al.  Carbon, hydrogen and nitrogen isotopes in solvent-extractable organic matter from carbonaceous chondrites , 1982 .

[18]  W. W Duley.  Interstellar chemistry , 1984 .

[19]  Carleton B. Moore,et al.  Total nitrogen and carbon abundances in carbonaceous chondrites , 1971 .

[20]  S. Pizzarello,et al.  Molecular and isotopic analyses of the hydroxy acids, dicarboxylic acids, and hydroxicarboxylic acids of the Murchison meteorite. , 1993, Geochimica et cosmochimica acta.

[21]  P. S. Goel,et al.  Nitrogen in stone meteorites and terrestrial standards , 1983 .

[22]  E. Anders,et al.  Meteorites and the Early Solar System , 1971 .

[23]  S. Pizzarello,et al.  Unusual stable isotope ratios in amino acid and carboxylic acid extracts from the Murchison meteorite , 1987, Nature.

[24]  R Shipp,et al.  Isotopic characterisation of kerogen-like material in the Murchison carbonaceous chondrite. , 1987, Geochimica et cosmochimica acta.

[25]  S. Pizzarello,et al.  Isotopic analyses of amino acids from the Murchison meteorite. , 1991, Geochimica et cosmochimica acta.

[26]  L. Fuchs,et al.  Mineralogy, mineral-chemistry, and composition of the Murchison (C2) meteorite , 1973 .

[27]  S. Macko,et al.  Carbon isotope composition of individual amino acids in the Murchison meteorite , 1990, Nature.

[28]  P. Wannier.  Nuclear Abundances and Evolution of the Interstellar Medium , 1980 .

[29]  Dale P. Cruikshank,et al.  Organic matter in carbonaceous chondrites, planetary satellites, asteroids and comets , 1988 .

[30]  D. Black.  Deuterium in the early solar system , 1973 .

[31]  S. Pizzarello,et al.  Amino acids in meteorites. , 1983, Advances in space research : the official journal of the Committee on Space Research.

[32]  Detection of Interstellar Methylamine by its 2_{02}->1_{10} A_{a^{-}} State Transition , 1974 .

[33]  S. Pizzarello,et al.  Isotopic and molecular analyses of hydrocarbons and monocarboxylic acids of the Murchison meteorite. , 1992, Geochimica et cosmochimica acta.

[34]  J. Vallentyne.  Biogeochemistry of organic matter—II Thermal reaction kinetics and transformation products of amino compounds , 1964 .

[35]  P. E. Hare,et al.  Chromatographic analysis of amino acids and primary amines with o-phthalaldehyde detection. , 1977, Analytical biochemistry.

[36]  Sherwood Chang,et al.  Organic matter in meteorites: molecular and isotopic analyses of the Murchison meteorite. , 1993 .