Protection of Escherichia coli ribosomes against streptomycin by purified initiation factors.

observable with 30-S subunits although the F,-F, association exerts the most potent effect, this effect being even more pronounced by the further addition of F,. The competing effect exerted by factors toward streptomycin binding does not manifest itself at low temperatures. Elongation factors have no effect. In line with these results, it was also found that combination of the three initiation factors can protect ribosomes against the streptomycin-induced release of initiator tRNA which

[1]  R. Miskin,et al.  Inactivation and reactivation of ribosomal subunits: amino acyl-transfer RNA binding activity of the 30 s subunit of Escherichia coli. , 1971, Journal of molecular biology.

[2]  F. Baralle,et al.  Studies on dissociation factor of bacterial ribosomes: effect of antibiotics. , 1971, Biochimica et Biophysica Acta.

[3]  A. Ghysen,et al.  Sensitivity and resistance to streptomycin in relation with factor‐mediated dissociation of ribosomes , 1971, FEBS letters.

[4]  M. Grunberg‐Manago,et al.  Streptomycin induced release of fMet-tRNA from the ribosomal initiation complex. , 1971, Biochemical and biophysical research communications.

[5]  B. D. Davis,et al.  Activity of Initiation Factor F3 in Dissociating Escherichia coli Ribosomes , 1970, Nature.

[6]  S. Ochoa,et al.  Purification and Properties of Initiation Factor F3 , 1970, Nature.

[7]  L. Bosch,et al.  Dissociation of 70 S E. coli ribosomes induced by a ribosomal factor (DF). Electrophoretic studies of the ribosomal particles , 1970, FEBS letters.

[8]  Z. Vogel,et al.  Ribosome activation and the binding of dihydrostreptomycin: effect of polynucleotides and temperature on activation. , 1970, Journal of molecular biology.

[9]  R. Thach,et al.  A new method for the purification of initiation factor F2 in high yield, and an estimation of stoichiometry in the binding reaction. , 1970, The Journal of biological chemistry.

[10]  J. Modolell,et al.  Breakdown by streptomycin of initiation complexes formed on ribosomes of Escherichia coli. , 1970, Proceedings of the National Academy of Sciences of the United States of America.

[11]  Y. Groner,et al.  Fractionation of translation initiation factor B (F3) into cistron‐specific species , 1970, FEBS letters.

[12]  M. Grunberg‐Manago,et al.  Interaction between Guanosine Derivatives and Factors involved in the Initiation of Protein Synthesis , 1970, Nature.

[13]  P. Spitnik-elson,et al.  A new approach to the fractionation of ribosomal protein , 1970, FEBS letters.

[14]  J. Modolell,et al.  Mechanism of Inhibition of Ribosomes by Streptomycin , 1969, Nature.

[15]  J. Lelong,et al.  Purification of initiator C from Escherichia coli: a protein which binds messenger RNA and initiator tRNA to the 30 s ribosome. , 1969, Journal of molecular biology.

[16]  S. Ochoa,et al.  Polypeptide chain initiation in E. coli: studies on the function of initiation factor F1. , 1969, Proceedings of the National Academy of Sciences of the United States of America.

[17]  J. Hershey,et al.  Purification and Properties of Initiation Factor f-1 , 1969, Nature.

[18]  M. Nomura,et al.  Identification and Functional Characterization of the Protein controlled by the Streptomycin-resistant Locus in E. coli , 1969, Nature.

[19]  E. Ron,et al.  A factor required for ribosome dissociation in Escherichia coli. , 1968, Proceedings of the National Academy of Sciences of the United States of America.

[20]  F. Gros,et al.  Function of Three Protein Factors and Ribosomal Subunits in the Initiation of Protein Synthesis in E. coli , 1968, Nature.

[21]  D. Schlessinger,et al.  Mechanism of action of streptomycin in E. coli: interruption of the ribosome cycle at the initiation of protein synthesis. , 1968, Proceedings of the National Academy of Sciences of the United States of America.

[22]  Y. Tanaka,et al.  Binding of dihydrostreptomycin to ribosomal subunits. , 1968, Journal of molecular biology.

[23]  R. Gesteland,et al.  In vitro Synthesis of Bacteriophage Lysozyme , 1967, Nature.