Cushioned-Density Gradient Ultracentrifugation (C-DGUC): A Refined and High Performance Method for the Isolation, Characterization, and Use of Exosomes.

[1]  Patrizia Agostinis,et al.  EV-TRACK: transparent reporting and centralizing knowledge in extracellular vesicle research , 2017, Nature Methods.

[2]  Monika Pietrowska,et al.  Proteomic analysis of exosomal cargo: the challenge of high purity vesicle isolation. , 2016, Molecular bioSystems.

[3]  Henrik J Johansson,et al.  Ultrafiltration with size-exclusion liquid chromatography for high yield isolation of extracellular vesicles preserving intact biophysical and functional properties. , 2015, Nanomedicine : nanotechnology, biology, and medicine.

[4]  S. Kourembanas Exosomes: vehicles of intercellular signaling, biomarkers, and vectors of cell therapy. , 2015, Annual review of physiology.

[5]  Joshua N Leonard,et al.  Therapeutic applications of extracellular vesicles: clinical promise and open questions. , 2015, Annual review of pharmacology and toxicology.

[6]  A. Möller,et al.  Optimized exosome isolation protocol for cell culture supernatant and human plasma , 2015, Journal of extracellular vesicles.

[7]  L. O’Driscoll,et al.  Biological properties of extracellular vesicles and their physiological functions , 2015, Journal of extracellular vesicles.

[8]  A. Brisson,et al.  High-speed centrifugation induces aggregation of extracellular vesicles , 2015, Journal of extracellular vesicles.

[9]  Richard J Simpson,et al.  A protocol for exosome isolation and characterization: evaluation of ultracentrifugation, density-gradient separation, and immunoaffinity capture methods. , 2015, Methods in molecular biology.

[10]  C. Théry,et al.  Biogenesis, secretion, and intercellular interactions of exosomes and other extracellular vesicles. , 2014, Annual review of cell and developmental biology.

[11]  Winston Patrick Kuo,et al.  Current methods for the isolation of extracellular vesicles , 2013, Biological chemistry.

[12]  J. Lötvall,et al.  Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells , 2007, Nature Cell Biology.

[13]  Zongyi Hu,et al.  Immunization with hepatitis C virus-like particles results in control of hepatitis C virus infection in chimpanzees , 2007, Proceedings of the National Academy of Sciences.

[14]  Aled Clayton,et al.  Isolation and Characterization of Exosomes from Cell Culture Supernatants and Biological Fluids , 2006, Current protocols in cell biology.

[15]  J. Le Pecq,et al.  Production and characterization of clinical grade exosomes derived from dendritic cells. , 2002, Journal of immunological methods.

[16]  R. Johnstone,et al.  Vesicle formation during reticulocyte maturation. Association of plasma membrane activities with released vesicles (exosomes). , 1987, The Journal of biological chemistry.