Ferrimonas marina sp. nov.

A novel Ferrimonas species is described on the basis of phenotypic, chemotaxonomic and phylogenetic studies. Four halophilic organisms were isolated from marine sand and marine macroalgae samples by using high-pH marine agar 2216. An analysis of the nearly complete 16S rRNA gene sequences of these new isolates indicated that they were phylogenetically close (16S rRNA gene sequence similarity >99.5%, gyrB gene sequence similarity >97.8%), and were most closely related to Ferrimonas balearica (16S rRNA gene sequence similarity 97.1-97.3%, gyrB gene sequence similarity 84.4-85.0%). Chemotaxonomic data (major menaquinone MK7; major fatty acids C(16:0) and C(18:1)omega9c) supported the affiliation of the new isolates to the genus Ferrimonas. The results of physiological and biochemical tests allowed phenotypic differentiation of the isolates from F. balearica. It is therefore proposed that the new isolates represent a novel species with the name Ferrimonas marina sp. nov. and type strain A4D-4(T) (=MBIC06480(T)=DSM 16917(T)).

[1]  H. Oikawa,et al.  Shewanella marinintestina sp. nov., Shewanella schlegeliana sp. nov. and Shewanella sairae sp. nov., novel eicosapentaenoic-acid-producing marine bacteria isolated from sea-animal intestines. , 2003, International journal of systematic and evolutionary microbiology.

[2]  Sudhir Kumar,et al.  MEGA2: molecular evolutionary genetics analysis software , 2001, Bioinform..

[3]  B. Velimirov,et al.  Investigation of 0.2 µm filterable bacteria from the Western Mediterranean Sea using a molecular approach: dominance of potential starvation forms. , 2000, FEMS microbiology ecology.

[4]  J. Thompson,et al.  The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. , 1997, Nucleic acids research.

[5]  H. Sano,et al.  Identification of isoprenoid quinones by frit-FAB liquid chromatography–mass spectrometry for the chemotaxonomy of microorganisms. , 1997 .

[6]  S. Harayama,et al.  PCR Amplification and Direct Sequencing of gyrB Genes with Universal Primers and Their Application to the Detection and Taxonomic Analysis of Pseudomonas putida Strains , 1995 .

[7]  R. Murray,et al.  Determinative and cytological light microscopy , 1994 .

[8]  Yves Van de Peer,et al.  TREECON for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment , 1994, Comput. Appl. Biosci..

[9]  S. Goodison,et al.  16S ribosomal DNA amplification for phylogenetic study , 1991, Journal of bacteriology.

[10]  E. Myers,et al.  Basic local alignment search tool. , 1990, Journal of molecular biology.

[11]  Takayuki Ezaki,et al.  Fluorometric Deoxyribonucleic Acid-Deoxyribonucleic Acid Hybridization in Microdilution Wells as an Alternative to Membrane Filter Hybridization in which Radioisotopes Are Used To Determine Genetic Relatedness among Bacterial Strains , 1989 .

[12]  D. Lovley,et al.  Organic Matter Mineralization with Reduction of Ferric Iron in Anaerobic Sediments , 1986, Applied and environmental microbiology.

[13]  K. Komagata,et al.  Determination of DNA base composition by reversed-phase high-performance liquid chromatography , 1984 .

[14]  R. Holman,et al.  Pyrrolidides for mass spectrometric determination of the position of the double bond in monounsaturated fatty acids , 1974, Lipids.

[15]  James R. Cole,et al.  The Ribosomal Database Project (RDP-II): previewing a new autoaligner that allows regular updates and the new prokaryotic taxonomy , 2003, Nucleic Acids Res..

[16]  T. Sakane.  Viabilities of dried cultures of various bacteria after preservation for over 20 years and their prediction by the accelerated storage test , 1997 .

[17]  Wolfgang Ludwig,et al.  Ferrimonas balearica gen. nov., spec. nov., a New Marine Facultative Fe(III)-reducing Bacterium , 1995 .