Role of CD8+ Lymphocytes in Control and Clearance of Measles Virus Infection of Rhesus Monkeys

ABSTRACT The creation of an improved vaccine for global measles control will require an understanding of the immune mechanisms of measles virus containment. To assess the role of CD8+ cytotoxic T lymphocytes in measles virus clearance, rhesus monkeys were depleted of CD8+ lymphocytes by monoclonal anti-CD8 antibody infusion and challenged with wild-type measles virus. The CD8+ lymphocyte-depleted animals exhibited a more extensive rash, higher viral loads at the peak of virus replication, and a longer duration of viremia than did the control antibody-treated animals. These findings indicate a central role for CD8+ lymphocytes in the control of measles virus infections and the importance of eliciting a cell-mediated immune response in new measles vaccine strategies.

[1]  T. Quinn,et al.  Prolonged measles virus shedding in human immunodeficiency virus-infected children, detected by reverse transcriptase-polymerase chain reaction. , 2001, The Journal of infectious diseases.

[2]  H. McClure,et al.  Sensitive and robust one-tube real-time reverse transcriptase-polymerase chain reaction to quantify SIV RNA load: comparison of one- versus two-enzyme systems. , 2000, AIDS research and human retroviruses.

[3]  F. Polack,et al.  Successful DNA immunization against measles: Neutralizing antibody against either the hemagglutinin or fusion glycoprotein protects rhesus macaques without evidence of atypical measles , 2000, Nature Medicine.

[4]  B. Murphy,et al.  Measles virus infection in rhesus macaques: altered immune responses and comparison of the virulence of six different virus strains. , 1999, The Journal of infectious diseases.

[5]  M. Manchester,et al.  Measles Virus Infection in a Transgenic Model Virus-Induced Immunosuppression and Central Nervous System Disease , 1999, Cell.

[6]  J. Schmitz,et al.  A nonhuman primate model for the selective elimination of CD8+ lymphocytes using a mouse-human chimeric monoclonal antibody. , 1999, The American journal of pathology.

[7]  G. Rall,et al.  Immune Response-Mediated Protection of Adult but Not Neonatal Mice from Neuron-Restricted Measles Virus Infection and Central Nervous System Disease , 1999, Journal of Virology.

[8]  D. Montefiori,et al.  Control of viremia in simian immunodeficiency virus infection by CD8+ lymphocytes. , 1999, Science.

[9]  H. Whittle,et al.  Antigen-Specific Expansion of Cytotoxic T Lymphocytes in Acute Measles Virus Infection , 1999, Journal of Virology.

[10]  H. Whittle,et al.  Ex vivo analysis of cytotoxic T lymphocytes to measles antigens during infection and after vaccination in Gambian children. , 1998, The Journal of clinical investigation.

[11]  Y. Maldonado,et al.  Deficiency of the humoral immune response to measles vaccine in infants immunized at age 6 months. , 1998, JAMA.

[12]  H. Whittle,et al.  Human leukocyte antigen class I- and class II-restricted cytotoxic T lymphocyte responses to measles antigens in immune adults. , 1998, The Journal of infectious diseases.

[13]  G. Landucci,et al.  Measles virus-specific functional antibody responses and viremia during acute measles. , 1994, The Journal of infectious diseases.

[14]  D. Griffin,et al.  Immune activation during measles: beta 2-microglobulin in plasma and cerebrospinal fluid in complicated and uncomplicated disease. , 1992, The Journal of infectious diseases.

[15]  L. Kaplan,et al.  Severe measles in immunocompromised patients. , 1992, JAMA.

[16]  Robert T. Chen,et al.  Measles antibody: reevaluation of protective titers. , 1990, The Journal of infectious diseases.

[17]  D. Griffin,et al.  Natural killer cell activity during measles , 1990, Clinical and experimental immunology.

[18]  A. Osterhaus,et al.  The predominance of CD8+ T cells after infection with measles virus suggests a role for CD8+ class I MHC-restricted cytotoxic T lymphocytes (CTL) in recovery from measles. Clonal analyses of human CD8+ class I MHC-restricted CTL. , 1990, Journal of immunology.

[19]  D. Griffin,et al.  Immune activation during measles: interferon-gamma and neopterin in plasma and cerebrospinal fluid in complicated and uncomplicated disease. , 1990, The Journal of infectious diseases.

[20]  A. Sugiura,et al.  Marmoset lymphoblastoid cells as a sensitive host for isolation of measles virus , 1990, Journal of virology.

[21]  D. Griffin,et al.  Immune activation in measles. , 1989, The New England journal of medicine.

[22]  D. Griffin,et al.  Prospective study of the magnitude and duration of changes in tuberculin reactivity during uncomplicated and complicated measles , 1987, The Pediatric infectious disease journal.

[23]  S. J. Cooper,et al.  Cellular immune responses during complicated and uncomplicated measles virus infections of man. , 1984, Clinical immunology and immunopathology.

[24]  V. Meulen,et al.  Demonstration of HLA restricted killer cells in patients with acute measles , 1979, Medical Microbiology and Immunology.

[25]  F. Burnet.  Measles as an index of immunological function. , 1968, Lancet.

[26]  K. Yoshida,et al.  Thymic aplasia with lymphopenia, plasma cells, and normal immunoglobulins. Relation to measles virus infection. , 1967, JAMA.

[27]  W. Winkelstein,et al.  Cooperative measles vaccine field trial. I. Clinical efficacy. , 1966, Pediatrics.

[28]  W. Winkelstein,et al.  COOPERATIVE MEASLES VACCINE FIELD TRIAL , 1966, Pediatrics.

[29]  S. Berkovich,et al.  EFFECTS OF MEASLES, GAMMA-GLOBULIN-MODIFIED MEASLES AND VACCINE MEASLES ON THE TUBERCULIN TEST. , 1964, The New England journal of medicine.

[30]  Lewis Jh.  Experiments of nature. , 1962 .

[31]  R. Good,et al.  Disturbances in gamma globulin synthesis as experiments of nature. , 1956, Pediatrics.

[32]  Global measles control and regional elimination, 1998-1999. , 1999, MMWR. Morbidity and mortality weekly report.

[33]  H. Takahashi,et al.  Detection and comparison of viral antigens in measles and rubella rashes. , 1996, Clinical infectious diseases : an official publication of the Infectious Diseases Society of America.

[34]  J. Sissons,et al.  Cytotoxic lymphocytes generated in vivo with acute measles virus infection. , 1985, Clinical immunology and immunopathology.

[35]  R. Good.  Use of a radioimmune assay in detection of measles antibodies in cerebrospinal fluid and serum. , 1977, Acta neurologica Scandinavica. Supplementum.

[36]  Good Ra.  Use of a radioimmune assay in detection of measles antibodies in cerebrospinal fluid and serum. , 1977 .