Microsatellite development and survey of genetic variation in skipjack tuna Katsuwonus pelamis

A survey of five newly developed microsatellite DNA markers in skipjack tuna Katsuwonus pelamis revealed high levels of polymorphism in two samples off the west coast of India (Minicoy Island and Kochi coast) and one sample off the Japan coast. Although significant differentiation (P < 0·01) in the number of specific alleles was observed between Minicoy and Kochi samples, the F-statistics values among the samples were very low (average = 0·0014) and not significant (P = 0·284).

[1]  N. Taniguchi,et al.  Genetic variation in skipjack tuna Katsuwonus pelamis(L.) using PCR-RFLP analysis of the mitochondrial DNA D-loop region , 2006 .

[2]  J. Durand,et al.  Nuclear and mitochondrial DNA markers indicate unidirectional gene flow of Indo-Pacific to Atlantic bigeye tuna (Thunnus obesus) populations, and their admixture off southern Africa , 2005 .

[3]  J. Wright,et al.  Microsatellite DNA in fishes , 1997, Reviews in Fish Biology and Fisheries.

[4]  Daniel W. Fuller,et al.  Movements, behavior, and habitat selection of bigeye tuna (Thunnus obesus) in the eastern equatorial Pacific, ascertained through archival tags , 2002 .

[5]  Peter J. S. Smith,et al.  Genetic divergence of kingfish from Japan, Australia and New Zealand inferred by microsatellite DNA and mitochondrial DNA control region markers , 2001 .

[6]  P. Grewe,et al.  Population structure of yellowfin tuna (Thunnusalbacares) in the western Pacific Ocean, inferred from microsatellite loci , 2001 .

[7]  C. Deschepper,et al.  M13-tailed primers improve the readability and usability of microsatellite analyses performed with two different allele-sizing methods. , 2001, BioTechniques.

[8]  Markus Schuelke,et al.  An economic method for the fluorescent labeling of PCR fragments , 2000, Nature Biotechnology.

[9]  G. Hewitt,et al.  Stock composition in North Atlantic populations of whiting using microsatellite markers , 1997 .

[10]  D. Cook,et al.  Isolation and characterization of microsatellite loci from red sea bream Pagrus major and detection in closely related species , 1997 .

[11]  K. Saitoh,et al.  Tissue Preservation and Total DNA Extraction form Fish Stored at Ambient Temperature Using Buffers Containing High Concentration of Urea , 1996 .

[12]  François Rousset,et al.  GENEPOP (version 1.2): population genetic software for exact tests and ecumenicism , 1995 .

[13]  D. Queller,et al.  Detection of highly polymorphic microsatellite loci in a species with little allozyme polymorphism , 1993, Molecular ecology.

[14]  Masatoshi Nei,et al.  Genetic Distance between Populations , 1972, The American Naturalist.

[15]  G. Raju.  Studies on the spawning of the oceanic skipjack Katsuwonus pelamis (Linnaeus) in Minicoy waters , 1964 .