Integrating Heterogeneous Odor Response Data into a Common Response Model: A DoOR to the Complete Olfactome

We have developed a new computational framework for merging odor response data sets from heterogeneous studies, creating a consensus metadatabase, the database of odor responses (DoOR). As a result, we obtained a functional atlas of all available odor responses in Drosophila melanogaster. Both the program and the data set are freely accessible and downloadable on the Internet (http://neuro.uni-konstanz.de/DoOR). The procedure can be adapted to other species, thus creating a family of “olfactomes” in the near future. Drosophila melanogaster was chosen because of all species this one is closest to having the complete olfactome characterized, with the highest number of deorphanized receptors available. The database guarantees long-term stability (by offering time-stamped, downloadable versions), up-to-date accuracy (by including new data sets as soon as they are published), and portability (for other species). We hope that this comprehensive repository of odor response profiles will be useful to the olfactory community and to computational neuroscientists alike.

[1]  Andrey Rzhetsky,et al.  A Spatial Map of Olfactory Receptor Expression in the Drosophila Antenna , 1999, Cell.

[2]  A. Fiala,et al.  Genetically Expressed Cameleon in Drosophila melanogaster Is Used to Visualize Olfactory Information in Projection Neurons , 2002, Current Biology.

[3]  C Giovanni Galizia,et al.  The molecular receptive range of an olfactory receptor in vivo (Drosophila melanogaster Or22a). , 2006, Journal of neurobiology.

[4]  Perry L. Miller,et al.  Olfactory Receptor Database: a metadata-driven automated population from sources of gene and protein sequences , 2002, Nucleic Acids Res..

[5]  John R. Carlson,et al.  The molecular basis of CO2 reception in Drosophila , 2007, Proceedings of the National Academy of Sciences.

[6]  Leslie B. Vosshall,et al.  Variant Ionotropic Glutamate Receptors as Chemosensory Receptors in Drosophila , 2009, Cell.

[7]  Gene H. Golub,et al.  Missing value estimation for DNA microarray gene expression data: local least squares imputation , 2005, Bioinform..

[8]  A. S. French,et al.  Two interacting olfactory transduction mechanisms have linked polarities and dynamics in Drosophila melanogaster antennal basiconic sensilla neurons. , 2009, Journal of neurophysiology.

[9]  John R. Carlson,et al.  The Molecular Basis of Odor Coding in the Drosophila Larva , 2005, Neuron.

[10]  Daniela Pelz.  Functional characterization of Drosophila melanogaster Olfactory Receptor Neurons , 2005 .

[11]  Gustavo Glusman,et al.  The complete human olfactory subgenome. , 2001, Genome research.

[12]  R. Axel,et al.  A novel multigene family may encode odorant receptors: A molecular basis for odor recognition , 1991, Cell.

[13]  John R. Carlson,et al.  Coexpression of Two Functional Odor Receptors in One Neuron , 2005, Neuron.

[14]  C Giovanni Galizia,et al.  Olfactory Information Processing in the Drosophila Antennal Lobe: Anything Goes? , 2008, The Journal of Neuroscience.

[15]  John R Carlson,et al.  The Molecular Basis of Odor Coding in the Drosophila Antenna , 2004, Cell.

[16]  Gisbert Schneider,et al.  Predicting olfactory receptor neuron responses from odorant structure , 2007, Chemistry Central journal.

[17]  John R. Carlson,et al.  Integrating the Molecular and Cellular Basis of Odor Coding in the Drosophila Antenna , 2003, Neuron.

[18]  Menzel,et al.  The role of glomeruli in the neural representation of odours: results from optical recording studies. , 2001, Journal of insect physiology.

[19]  A. Malhotra,et al.  The Molecular Basis for Ligand Specificity in a Mouse Olfactory Receptor , 2007, Journal of Biological Chemistry.

[20]  John R. Carlson,et al.  Odor Coding in the Drosophila Antenna , 2001, Neuron.

[21]  Aidan Kiely,et al.  Drosophila odorant receptors are novel seven transmembrane domain proteins that can signal independently of heterotrimeric G proteins. , 2008, Insect biochemistry and molecular biology.

[22]  L. Vosshall,et al.  Influence of odorant receptor repertoire on odor perception in humans and fruit flies , 2007, Proceedings of the National Academy of Sciences.

[23]  Gisbert Schneider,et al.  Processing and classification of chemical data inspired by insect olfaction , 2007, Proceedings of the National Academy of Sciences.

[24]  Peter J. Clyne,et al.  Odor Coding in a Model Olfactory Organ: TheDrosophila Maxillary Palp , 1999, The Journal of Neuroscience.

[25]  Jing W. Wang,et al.  Select Drosophila glomeruli mediate innate olfactory attraction and aversion , 2009, Nature.

[26]  Bill S Hansson,et al.  Novel natural ligands for Drosophila olfactory receptor neurones , 2003, Journal of Experimental Biology.

[27]  Zito Tiziano.  G-Node: An Integrated Tool-Sharing Platform to Support Cellular and Systems Neurophysiology in the Age of Global Neuroinformatics , 2008 .

[28]  A. Wong,et al.  Two-Photon Calcium Imaging Reveals an Odor-Evoked Map of Activity in the Fly Brain , 2003, Cell.

[29]  L. Vosshall,et al.  Molecular architecture of smell and taste in Drosophila. , 2007, Annual review of neuroscience.

[30]  C Giovanni Galizia,et al.  Processing of Odor Mixtures in the Drosophila Antennal Lobe Reveals both Global Inhibition and Glomerulus-Specific Interactions , 2007, The Journal of Neuroscience.

[31]  P. Hiesinger,et al.  Three‐dimensional reconstruction of the antennal lobe in Drosophila melanogaster , 1999, The Journal of comparative neurology.

[32]  Randolf Menzel,et al.  Sensory Memory for Odors Is Encoded in Spontaneous Correlated Activity Between Olfactory Glomeruli , 2006, Neural Computation.

[33]  John R. Carlson,et al.  Translation of Sensory Input into Behavioral Output via an Olfactory System , 2008, Neuron.

[34]  Leslie B. Vosshall,et al.  Or83b Encodes a Broadly Expressed Odorant Receptor Essential for Drosophila Olfaction , 2004, Neuron.

[35]  Barry J. Dickson,et al.  Molecular, Anatomical, and Functional Organization of the Drosophila Olfactory System , 2005, Current Biology.

[36]  John R. Carlson,et al.  Coding of Odors by a Receptor Repertoire , 2006, Cell.