Spherization of red blood cells and platelet margination in COPD patients

Red blood cells (RBCs) in pathological situations undergo biochemical and conformational changes, leading to alterations in rheology involved in cardiovascular events. The shape of RBCs in volunteers and stable and exacerbated chronic obstructive pulmonary disease (COPD) patients was analyzed. The effects of RBC spherization on platelet transport (displacement in the flow field caused by their interaction with RBCs) were studied in vitro and by numerical simulations. RBC spherization was observed in COPD patients compared with volunteers. In in vitro experiments at a shear rate of 100 s−1, treatment of RBCs with neuraminidase induced greater sphericity, which mainly affected platelet aggregates without changing aggregate size. At 400 s−1, neuraminidase treatment changes both the size of the aggregates and the number of platelet aggregates. Numerical simulations indicated that RBC spherization induces an increase of the platelet mean square displacement, which is traditionally linked to the platelet diffusion coefficient. RBCs of COPD patients are more spherical than healthy volunteers. Experimentally, RBC spherization induces increased platelet transport to the wall. Additional studies are needed to understand the link between the effect of RBCs on platelet transport and the increased cardiovascular events observed in COPD patients.

[1]  Orestis Malaspinas,et al.  Palabos: Parallel Lattice Boltzmann Solver , 2020, Comput. Math. Appl..

[2]  Bastien Chopard,et al.  Digital blood in massively parallel CPU/GPU systems for the study of platelet transport , 2019, Interface Focus.

[3]  Bastien Chopard,et al.  Bridging the computational gap between mesoscopic and continuum modeling of red blood cells for fully resolved blood flow , 2019, J. Comput. Phys..

[4]  J. Weisel,et al.  Red blood cells: the forgotten player in hemostasis and thrombosis , 2019, Journal of thrombosis and haemostasis : JTH.

[5]  J. Hochman,et al.  Circulating monocyte-platelet aggregates are a robust marker of platelet activity in cardiovascular disease. , 2019, Atherosclerosis.

[6]  D. Lillicrap,et al.  Fifty years new , 2018, Journal of thrombosis and haemostasis : JTH.

[7]  George Em Karniadakis,et al.  Quantifying Platelet Margination in Diabetic Blood Flow , 2018, bioRxiv.

[8]  N. S. Gowert,et al.  Platelet-RBC interaction mediated by FasL/FasR induces procoagulant activity important for thrombosis , 2018, The Journal of clinical investigation.

[9]  V. Genkel,et al.  Association between Carotid Wall Shear Rate and Arterial Stiffness in Patients with Hypertension and Atherosclerosis of Peripheral Arteries , 2018, International journal of vascular medicine.

[10]  Guy Courbebaisse,et al.  A physical description of the adhesion and aggregation of platelets , 2015, Royal Society Open Science.

[11]  Erlend Magnus Viggen,et al.  The Lattice Boltzmann Method , 2017 .

[12]  M. Gąsior,et al.  The Prognostic Role of Red Blood Cell Distribution Width in Coronary Artery Disease: A Review of the Pathophysiology , 2015, Disease markers.

[13]  Scott L Diamond,et al.  Platelet dynamics in three-dimensional simulation of whole blood. , 2014, Biophysical journal.

[14]  Jonathan B. Freund,et al.  Simulation of Platelet, Thrombus and Erythrocyte Hydrodynamic Interactions in a 3D Arteriole with In Vivo Comparison , 2013, PloS one.

[15]  George Em Karniadakis,et al.  Blood flow in small tubes: quantifying the transition to the non-continuum regime , 2013, Journal of Fluid Mechanics.

[16]  J. Wautier,et al.  Molecular basis of erythrocyte adhesion to endothelial cells in diseases. , 2013, Clinical hemorheology and microcirculation.

[17]  Michael D. Graham,et al.  Margination and segregation in confined flows of blood and other multicomponent suspensions , 2012 .

[18]  S. Gangopadhyay,et al.  Lipids of Erythrocyte Membranes of COPD Patients: A Quantitative and Qualitative Study , 2012, COPD.

[19]  S. Diamond,et al.  Multiscale Systems Biology and Physics of Thrombosis Under Flow , 2012, Annals of Biomedical Engineering.

[20]  Y. Colin,et al.  Red blood cell phosphatidylserine exposure is responsible for increased erythrocyte adhesion to endothelium in central retinal vein occlusion , 2011, Journal of thrombosis and haemostasis : JTH.

[21]  Aaron L. Fogelson,et al.  Analysis of mechanisms for platelet near-wall excess under arterial blood flow conditions , 2011, Journal of Fluid Mechanics.

[22]  F. N. van de Vosse,et al.  The influence of boundary conditions on wall shear stress distribution in patients specific coronary trees. , 2011, Journal of biomechanics.

[23]  Takaji Inamuro,et al.  Lift generation by a two-dimensional symmetric flapping wing , 2010 .

[24]  Laura C Rodrigues,et al.  Prevalence of major comorbidities in subjects with COPD and incidence of myocardial infarction and stroke: a comprehensive analysis using data from primary care , 2010, Thorax.

[25]  R. Hubbard,et al.  Increased risk of myocardial infarction and stroke following exacerbation of COPD. , 2010, Chest.

[26]  O. Gajic,et al.  A postmortem analysis of major causes of early death in patients hospitalized with COPD exacerbation. , 2009, Chest.

[27]  J. Vincent,et al.  Neuraminidase alters red blood cells in sepsis , 2009, Critical care medicine.

[28]  E. Edelman,et al.  Prediction of the Localization of High-Risk Coronary Atherosclerotic Plaques on the Basis of Low Endothelial Shear Stress: An Intravascular Ultrasound and Histopathology Natural History Study , 2008, Circulation.

[29]  K Zouaoui Boudjeltia,et al.  Assessment of erythrocyte shape by flow cytometry techniques , 2006, Journal of Clinical Pathology.

[30]  Judith K Jones,et al.  Cardiovascular disease in patients with chronic obstructive pulmonary disease, Saskatchewan Canada cardiovascular disease in COPD patients. , 2006, Annals of epidemiology.

[31]  J. Vincent,et al.  Alterations of red blood cell shape and sialic acid membrane content in septic patients , 2003, Critical care medicine.

[32]  K. Ohtomo,et al.  Vector analysis of the wall shear rate at the human aortoiliac bifurcation using cine MR velocity mapping. , 2002, AJR. American journal of roentgenology.

[33]  H. Goldsmith,et al.  Effect of red blood cells and their aggregates on platelets and white cells in flowing blood. , 1999, Biorheology.

[34]  M. T. Santini,et al.  Structural alterations in erythrocytes from patients with chronic obstructive pulmonary disease. , 1997, Haemostasis.

[35]  Y. C. Fung,et al.  Improved measurements of the erythrocyte geometry. , 1972, Microvascular research.

[36]  Goldsmith Hl,et al.  Red cell motions and wall interactions in tube flow. , 1971 .

[37]  H. Goldsmith,et al.  Red cell motions and wall interactions in tube flow. , 1971, Federation proceedings.