Time- and dose-resolved proteome of PM2.5 exposure induced lung injury and repair in rat.

In recent years, airborne fine particulate matter (PM2.5) is drawing more public attention due to various of physicochemical features and pathological damages by epidemiological and clinical studies. However, the mechanism of PM2.5-exposure induced lung injury has not been fully characterized. Here, we established a PM2.5-induced rat injury model for short-term and long-term at different concentrations. We employed Fast-seq technique to profile 6,316 proteins and catTFRE approach to profile 387 TFs in lung tissue. In short-term exposure, we elucidated gradually upregulated proteins enriched in response to oxidative stress, phagosome, and ECM-receptor interaction pathway. Long-term exposure mainly showed immune response pathway consisting of increased lymphocytes and cytokines. Intriguingly, we found immune related proteins were recoverable at short-term exposure. During the process of PM2.5 exposure, upregulated proteins presented dose-dependence in lung, including stress response at low-dose, lighter immune response at middle-dose, and severe inflammatory response at high-dose. This dataset provides a rich resource to facilitate the understanding of PM2.5-induced lung damage and repair mechanism.

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