Disruption of native and recombinant Escherichia coli in a high‐pressure homogenizer

The disruption of native and recombinant strains of Escherichia coli was studied using a high‐pressure homogenizer (Microfluidizer). The cells were grown in both batch and continuous fermentations. Cell suspensions ranging from 4 to 175 g dry wt/L were investigated at disruption pressures ranging from 30–95 MPa and at up to five passes. For both types of cells, the fraction of cells disrupted was dependent on the growth rate and concentration of the cells, the disruption pressure, and the number of passes through the disrupter. A model is presented that correlates the fractional disruption with these operating variables. The recombinant strain disrupted more readily than the native strain; 95 to 98% disruption of the former was achieved in two to three passes at a pressure of 95 MPa.

[1]  C. Robinson,et al.  Disruption of Candida utilis cells in high pressure flow devices * , 1981 .

[2]  H. Hustedt,et al.  Experiences with a 20 litre industrial bead mill for the disruption of microorganisms , 1983 .

[3]  Jeffrey H. Miller.  Experiments in molecular genetics , 1972 .

[4]  T. Hammond,et al.  A hydrodynamic mechanism for the disintegration of Saccharomyces cerevisiae in an industrial homogenizer , 1975 .

[5]  C. W. Robinson,et al.  New method of measuring cell‐wall rupture , 1979 .

[6]  C. B. Orsborn,et al.  Reactor properties of a high‐speed bead mill for microbial cell rupture , 1979 .

[7]  R. Koski,et al.  Controlled expression and purification of human immune interferon from high‐cell‐density fermentations of Saccharomyces cerevisiae , 1987, Biotechnology and bioengineering.

[8]  M. Kula,et al.  Enzyme yields from cells of brewer's yeast disrupted by treatment in a horizontal disintegrator , 1974, Biotechnology and bioengineering.

[9]  K. J. Orford,et al.  A 12.6-ms pulsar in Cygnus X-3 , 1985, Nature.

[10]  Yusuf Chisti,et al.  Disruption of microbial cells for intracellular products , 1986 .

[11]  D. A. Whitworth.  Letter: Hydrocarbon fermentation: protein and enzyme solubilization from C. lipolytica using an industrial homogenizer. , 1974, Biotechnology and bioengineering.

[12]  Peter Dunnill,et al.  Investigation of the unit operations involved in the continuous flow isolation of β‐galactosidase from Escherichia coli , 1978 .

[13]  J. Brookman,et al.  Mechanism of cell disintegration in a high pressure homogenizer , 1974 .

[14]  M. Lilly,et al.  Release of enzymes from bakers' yeast by disruption in an industrial homogenizer , 1971, Biotechnology and bioengineering.

[15]  J. Řeháček,et al.  Disintegration of microorganisms in an industrial horizontal mill of novel design , 1977 .