Dietary conjugated linoleic acid (CLA) for finishing Nile tilapia

A 8-week feeding trial was conducted to evaluate the nutritional value of dietary conjugated linoleic acid (CLA) for finishing Nile tilapia evaluating its effects on growth performance, whole body and filet compositions, fatty acid composition, plasma lipid parameters, filet texture, liquid loss and holding capacity. Triplicate groups of Nile tilapia were hand-fed until apparent satiation on diets in which CLA oil was supplemented at 0 (control), 5 or 10 g kg−1. No differences were observed on weight gain, feed intake, feed/gain ratio, protein efficiency ratio, hepatosomatic index, visceral fat, filet yield and survival between treatments. Fish-fed CLA diets had increased whole body crude protein and reduced filet lipid content. The total plasma cholesterol and triacylglycerol decreased with CLA supplementation. Neither 5 nor 10 g kg−1 dietary CLA improved liquid-holding capacity and filet texture when compared with fish fed the control diet. Fish-fed dietary CLA showed lower whole body and filet C18:3(n-6), C18:3(n-3) and C20:2(n-6) levels and higher levels of C18:0. CLA deposition in filet and whole body (16 mg–235 mg g−1 of tissue) are higher than presented in natural sources of CLA, proving to be a great product to increase CLA consumption by humans.

[1]  N. Bandarra,et al.  Time course deposition of conjugated linoleic acid in market size rainbow trout (Oncorhynchus mykiss) muscle , 2008 .

[2]  Wilson Massamitu Furuya,et al.  Ácido linoléico conjugado (CLA) em dietas para tilápia-do-nilo: desempenho produtivo, composição química e perfil de ácidos graxos , 2007 .

[3]  Wilson Massamitu Furuya,et al.  Deposição de ácido linoléico conjugado (CLA) em tilápias-do-nilo , 2007 .

[4]  N. Bandarra,et al.  Influence of conjugated linoleic acid on growth, lipid composition and hepatic lipogenesis in juvenile European sea bass (Dicentrarchus labrax) , 2007 .

[5]  S. Hur,et al.  A COMPARISON OF THE EFFECTS OF DIETARY CONJUGATED LINOLEIC ACID CONTENTS, CHOLESTEROL, LIPID OXIDATION AND DRIP LOSS IN PORK LOIN AND CHICKEN BREAST , 2007 .

[6]  M. Portillo,et al.  Effects of trans-10,cis-12 conjugated linoleic acid on cholesterol metabolism in hypercholesterolaemic hamsters , 2007, European journal of nutrition.

[7]  D. Tocher,et al.  Influence of dietary conjugated linoleic acid (CLA) and tetradecylthioacetic acid (TTA) on growth, lipid composition and key enzymes of fatty acid oxidation in liver and muscle of Atlantic cod (Gadus morhua L.) , 2007 .

[8]  N. Bandarra,et al.  Conjugated linoleic acid in diets for large-size rainbow trout (Oncorhynchus mykiss): effects on growth, chemical composition and sensory attributes , 2007, British Journal of Nutrition.

[9]  W. Suksombat,et al.  Effects of various levels of conjugated linoleic acid supplementation on fatty acid content and carcass composition of broilers. , 2007, Poultry science.

[10]  B. Watkins,et al.  Effects of dietary conjugated linoleic acids on hepatic and muscle lipids in hybrid striped bass , 2000, Lipids.

[11]  D. Tocher,et al.  Effect of dietary conjugated linoleic acid (CLA) on lipid composition, metabolism and gene expression in Atlantic salmon (Salmo salar) tissues. , 2006, Comparative biochemistry and physiology. Part A, Molecular & integrative physiology.

[12]  D. Richard,et al.  The effects of t10,c12 CLA isomer compared with c9,t11 CLA isomer on lipid metabolism and body composition in hamsters. , 2006, The Journal of nutritional biochemistry.

[13]  N. Bandarra,et al.  Effect of dietary conjugated linoleic acid on muscle, liver and visceral lipid deposition in rainbow trout juveniles (Oncorhynchus mykiss) , 2006 .

[14]  N. Bergeron,et al.  Lack of effect of dietary conjugated linoleic acids naturally incorporated into butter on the lipid profile and body composition of overweight and obese men. , 2005, The American journal of clinical nutrition.

[15]  N. Bandarra,et al.  Effects of dietary conjugated linoleic acid on growth, nutrient utilization, body composition, and hepatic lipogenesis in rainbow trout juveniles (Oncorhynchus mykiss) , 2005 .

[16]  D. Tocher,et al.  Influence of dietary conjugated linoleic acid (CLA) on lipid and fatty acid composition in liver and flesh of Atlantic salmon (Salmo salar). , 2005, Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology.

[17]  P. Henckel,et al.  Influence of dietary conjugated linoleic acid (CLA) and age at slaughtering on performance, slaughter- and meat quality, lipoproteins, and tissue deposition of CLA in barrows. , 2005, Meat science.

[18]  R. Schubring Changes in Texture, Water Holding Capacity, Colour and Thermal Stability of Frozen Cod (Gadus morhua) Fillets: Effect of Frozen Storage Temperature , 2005 .

[19]  J. Chardigny,et al.  Isomers of conjugated linoleic acid decrease plasma lipids and stimulate adipose tissue lipogenesis without changing adipose weight in post-prandial adult sedentary or trained Wistar rat. , 2004, The Journal of nutritional biochemistry.

[20]  M. Jung,et al.  Effect of conjugated linoleic acid isomers on insulin resistance and mRNA levels of genes regulating energy metabolism in high-fat-fed rats. , 2004, Nutrition.

[21]  P. Calder,et al.  Opposing effects of cis-9,trans-11 and trans-10,cis-12 conjugated linoleic acid on blood lipids in healthy humans. , 2004, The American journal of clinical nutrition.

[22]  B. Ruyter,et al.  Conjugated linoleic acid in diets for juvenile Atlantic salmon (Salmo salar); effects on fish performance, proximate composition, fatty acid and mineral content , 2004 .

[23]  M. Hayashi,et al.  Effect of conjugated linoleic and docosahexaenoic acids on growth of juvenile tilapia Oreochromis niloticus , 2004 .

[24]  G. Maiorano,et al.  Influence of dietary conjugated linoleic acids (CLA) and age at slaughtering on meat quality and intramuscular collagen in rabbits. , 2004, Meat science.

[25]  R. Twibell,et al.  Effects of dietary conjugated linoleic acids and total dietary lipid concentrations on growth responses of juvenile channel catfish, Ictalurus punctatus , 2003 .

[26]  M. Kushiro,et al.  Activity and mRNA levels of enzymes involved in hepatic fatty acid synthesis and oxidation in mice fed conjugated linoleic acid. , 2003, Biochimica et biophysica acta.

[27]  G. Jahreis,et al.  Effect of dietary conjugated linoleic acids on the distribution of fatty acids in serum lipoprotein fractions and different tissues of growing pigs. , 2002, Journal of animal physiology and animal nutrition.

[28]  M. McIntosh,et al.  Isomer-specific effects of conjugated linoleic acid (CLA) on adiposity and lipid metabolism. , 2002, The Journal of nutritional biochemistry.

[29]  Luiz Edivaldo Pezzato,et al.  Digestibilidade Aparente de Ingredientes pela Tilápia do Nilo (Oreochromis niloticus) , 2002 .

[30]  K. Eder,et al.  Trans-10,cis-12 conjugated linoleic acid suppresses the desaturation of linoleic and alpha-linolenic acids in HepG2 cells. , 2002, The Journal of nutrition.

[31]  M. Katan,et al.  The decrease in body fat in mice fed conjugated linoleic acid is due to increases in energy expenditure and energy loss in the excreta. , 2002, The Journal of nutrition.

[32]  J. Mourot,et al.  Influence of dietary conjugated linoleic acid on growth, meat quality, lipogenesis, plasma leptin and physiological variables of lipid metabolism in rabbits. , 2002, Journal of animal science.

[33]  D. Ahn,et al.  Effect of dietary conjugated linoleic acid on the growth rate of live birds and on the abdominal fat content and quality of broiler meat. , 2002, Poultry science.

[34]  S. Joo,et al.  Effects of dietary conjugated linoleic acid on fatty acid composition, lipid oxidation, color, and water-holding capacity of pork loin. , 2002, Journal of animal science.

[35]  B. Watkins,et al.  Dietary conjugated linoleic acids and lipid source alter fatty acid composition of juvenile yellow perch, Perca flavescens. , 2001, The Journal of nutrition.

[36]  M. Cook,et al.  The biologically active isomers of conjugated linoleic acid. , 2001, Progress in lipid research.

[37]  M. Cook,et al.  CLA reduces antigen-induced histamine and PGE(2) release from sensitized guinea pig tracheae. , 2001, American journal of physiology. Regulatory, integrative and comparative physiology.

[38]  Yu Huang,et al.  Dietary conjugated linoleic acid mixture affects the activity of intestinal acyl coenzyme A: cholesterol acyltransferase in hamsters. , 2000, The British journal of nutrition.

[39]  R. H. Liu,et al.  trans-10,cis-12-conjugated linoleic acid isomer exhibits stronger oxyradical scavenging capacity than cis-9,trans-11-conjugated linoleic acid isomer. , 2000, Journal of agricultural and food chemistry.

[40]  Hyoun-ju Kim,et al.  Conjugated linoleic acid supplementation reduces adipose tissue by apoptosis and develops lipodystrophy in mice. , 2000, Diabetes.

[41]  M. Gómez-Guillén,et al.  Biological Characteristics Affect the Quality of Farmed Atlantic Salmon and Smoked Muscle , 2000 .

[42]  M. Cook,et al.  Mechanisms of Action of Conjugated Linoleic Acid: Evidence and Speculation , 2000 .

[43]  B. Tuchweber,et al.  An isomeric mixture of conjugated linoleic acids but not pure cis-9, trans-11-octadecadienoic acid affects body weight gain and plasma lipids in hamsters. , 2000, The Journal of nutrition.

[44]  D. Bauman,et al.  Dietary conjugated linoleic acids increase lean tissue and decrease fat deposition in growing pigs. , 1999, The Journal of nutrition.

[45]  K. Yamauchi,et al.  Dietary conjugated linoleic acid reciprocally modifies ketogenesis and lipid secretion by the rat liver , 1999, Lipids.

[46]  Yeonhwa Park,et al.  Evidence that the trans-10,cis-12 isomer of conjugated linoleic acid induces body composition changes in mice , 1999, Lipids.

[47]  K. Thompson,et al.  Dietary conjugated linoleic acids promote fatty streak formation in the C57BL/6 mouse atherosclerosis model , 1999, British Journal of Nutrition.

[48]  B. Choi,et al.  Accumulation of Conjugated Linoleic Acid (CLA) in Tissues of Fish Fed Diets Containing Various Levels of CLA , 1999 .

[49]  K. N. Lee,et al.  Conjugated linoleic acid decreases hepatic stearoyl-CoA desaturase mRNA expression. , 1998, Biochemical and biophysical research communications.

[50]  A. Schaefer,et al.  The effect of conjugated linoleic acid on fat to lean repartitioning and feed conversion in pigs , 1997 .

[51]  M. Cook,et al.  Effect of conjugated linoleic acid on body composition in mice , 1997, Lipids.

[52]  R. Nicolosi,et al.  Dietary conjugated linoleic acid reduces plasma lipoproteins and early aortic atherosclerosis in hypercholesterolemic hamsters. , 1997, Artery.

[53]  M. Cook,et al.  Conjugated linoleic acid is a growth factor for rats as shown by enhanced weight gain and improved feed efficiency. , 1994, The Journal of nutrition.

[54]  K. N. Lee,et al.  Conjugated linoleic acid and atherosclerosis in rabbits. , 1994, Atherosclerosis.

[55]  C. Miller,et al.  Immune modulation by altered nutrient metabolism: nutritional control of immune-induced growth depression. , 1993, Poultry science.

[56]  M. Pariza,et al.  Dietary sources of conjugated dienoic isomers of linoleic acid, a newly recognized class of anticarcinogens , 1992 .

[57]  C. Ip,et al.  Mammary cancer prevention by conjugated dienoic derivative of linoleic acid. , 1991, Cancer research.

[58]  M. Pariza,et al.  Inhibition of benzo(a)pyrene-induced mouse forestomach neoplasia by conjugated dienoic derivatives of linoleic acid. , 1990, Cancer research.

[59]  D. Tocher,et al.  The lipid composition and biochemistry of freshwater fish. , 1987, Progress in lipid research.

[60]  R. Levy,et al.  Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. , 1972, Clinical chemistry.

[61]  W. J. Dyer,et al.  A rapid method of total lipid extraction and purification. , 1959, Canadian journal of biochemistry and physiology.