Protein electrochemistry using graphene-based nano-assembly: an ultrasensitive electrochemical detection of protein molecules via nanoparticle-electrode collisions.

We describe a new electrochemical detection approach towards single protein molecules (microperoxidase-11, MP-11), which are attached to the surface of graphene nanosheets. The non-covalently functionalized graphene nanosheets exhibit enhanced electroactive surface area, where amplified redox current is produced when graphene nanosheets collide with the electrode.

[1]  Yuyuan Tian,et al.  Detection, counting, and imaging of single nanoparticles. , 2014, Analytical chemistry.

[2]  Wenrong Yang,et al.  Non-covalent surface modification of boron nitride nanotubes for enhanced catalysis. , 2013, Chemical communications.

[3]  Pradyumna S. Singh,et al.  Noise characteristics of nanoscaled redox-cycling sensors: investigations based on random walks. , 2013, Journal of the American Chemical Society.

[4]  J. L. Yang,et al.  Chemical mapping of a single molecule by plasmon-enhanced Raman scattering , 2013, Nature.

[5]  Scott N. Thorgaard,et al.  Single particle detection by area amplification: single wall carbon nanotube attachment to a nanoelectrode. , 2013, Journal of the American Chemical Society.

[6]  Pradyumna S. Singh,et al.  Single-molecule electrochemistry: present status and outlook. , 2013, Accounts of chemical research.

[7]  R. Crooks,et al.  Electrochemical detection of individual DNA hybridization events. , 2013, Lab on a chip.

[8]  Pradyumna S. Singh,et al.  Stochasticity in single-molecule nanoelectrochemistry: origins, consequences, and solutions. , 2012, ACS nano.

[9]  Vincent M Rotello,et al.  Nanoscale graphene oxide (nGO) as artificial receptors: implications for biomolecular interactions and sensing. , 2012, Journal of the American Chemical Society.

[10]  Richard G. Compton,et al.  Electron transfer kinetics at single nanoparticles , 2012 .

[11]  Richard G. Compton,et al.  The electrochemical detection of tagged nanoparticles via particle-electrode collisions: nanoelectroanalysis beyond immobilisation. , 2012, Chemical communications.

[12]  Peng Chen,et al.  How does a single Pt nanocatalyst behave in two different reactions? A single-molecule study. , 2012, Nano letters.

[13]  R. Compton,et al.  Gold nanoparticles show electroactivity: counting and sorting nanoparticles upon impact with electrodes. , 2012, Chemical communications.

[14]  P. Unwin,et al.  Electrochemistry at nanoscale electrodes: individual single-walled carbon nanotubes (SWNTs) and SWNT-templated metal nanowires. , 2011, ACS nano.

[15]  A. Bard,et al.  Electrochemistry of Single Nanoparticles via Electrocatalytic Amplification , 2010 .

[16]  Shun Mao,et al.  Specific Protein Detection Using Thermally Reduced Graphene Oxide Sheet Decorated with Gold Nanoparticle‐Antibody Conjugates , 2010, Advanced materials.

[17]  Filip Braet,et al.  Carbon nanomaterials in biosensors: should you use nanotubes or graphene? , 2010, Angewandte Chemie.

[18]  Chunhai Fan,et al.  Graphene oxide-facilitated electron transfer of metalloproteins at electrode surfaces. , 2010, Langmuir : the ACS journal of surfaces and colloids.

[19]  A. Bard.  Toward single enzyme molecule electrochemistry. , 2008, ACS nano.

[20]  Dehong Hu,et al.  Single-molecule fluorescence spectroelectrochemistry of cresyl violet. , 2008, Chemical communications.

[21]  H. Heering,et al.  Toward single-enzyme molecule electrochemistry: [NiFe]-hydrogenase protein film voltammetry at nanoelectrodes. , 2008, ACS nano.

[22]  T. Albrecht,et al.  Single-molecule electron transfer in electrochemical environments. , 2008, Chemical reviews.

[23]  P. Unwin,et al.  Trace level cyclic voltammetry facilitated by single-walled carbon nanotube network electrodes. , 2007, Journal of the American Chemical Society.

[24]  Xiaoyin Xiao,et al.  Observing single nanoparticle collisions at an ultramicroelectrode by electrocatalytic amplification. , 2007, Journal of the American Chemical Society.

[25]  S. Lemay,et al.  Time-resolved electrochemical detection of discrete adsorption events. , 2004, Journal of the American Chemical Society.

[26]  L. Gorton,et al.  Diffusionless electron transfer of microperoxidase-11 on gold electrodes , 1999 .

[27]  M. Heyrovský,et al.  Polarography and voltammetry of ultrasmall colloids : introduction to a new field , 1995 .

[28]  Charles N. Reilley,et al.  Thin-layer electrochemistry: steady-state methods of studying rate processes , 1965 .

[29]  W. Marsden.  I and J , 2012 .