Critical review on retrospective and prospective changes in antifungal susceptibility testing for dermatophytes

Antifungal susceptibility testing is a progressive field of mycology with respect to dermatophytes as well as non‐dermatophytes. Documentation and approval regarding standardisation of in vitro susceptibility testing have been done by different governing bodies such as Clinical and Laboratory Standards Institute, European Committee on Antimicrobial Susceptibility Testing and British Society of Antimicrobial Chemotherapy. The process of standardisation and approval started in 1985 with certain amendments in 2002 and 2004. In case of dermatophytes, antifungal susceptibility testing protocol has been approved recently in 2008; however, the approved standardisation also needs certain amendments/modifications due to the limitations of an existing method. In the present review, we have compiled these standardisation techniques along with the limitations and advantages and the amendments that have been proposed at different time periods by different researchers and regulatory agencies.

[1]  M. Balouiri,et al.  Methods for in vitro evaluating antimicrobial activity: A review☆ , 2015, Journal of pharmaceutical analysis.

[2]  G. Indira In Vitro Antifungal Susceptibility Testing of 5 Antifungal Agents against Dermatophytic Species by CLSI (M38-A) Micro Dilution Method , 2014 .

[3]  J. D. Del Rosso The role of topical antifungal therapy for onychomycosis and the emergence of newer agents. , 2014, The Journal of clinical and aesthetic dermatology.

[4]  G. González,et al.  Susceptibility of Dermatophytes to Thiabendazole Using CLSI Broth Macrodilution , 2012, ISRN dermatology.

[5]  Surbhi Leekha,et al.  General Principles of Antimicrobial Therapy , 2011, Mayo Clinic proceedings.

[6]  R. Misra,et al.  Comparison of the activities of amphotericin B, itraconazole, and voriconazole against clinical and environmental isolates of Aspergillus species. , 2011, Indian journal of pathology & microbiology.

[7]  M. Ghannoum,et al.  Agar-Based Disk Diffusion Assay for Susceptibility Testing of Dermatophytes , 2010, Journal of Clinical Microbiology.

[8]  I. Lenci,et al.  In vitro activity of Tachyplesin III alone and in combination with terbinafine against clinical isolates of dermatophytes , 2009, Peptides.

[9]  M. Ghannoum,et al.  Susceptibility testing of dermatophytes , 2009 .

[10]  N. Martinez-Rossi,et al.  In vitro antifungal drug susceptibilities of dermatophytes microconidia and arthroconidia. , 2008, The Journal of antimicrobial chemotherapy.

[11]  J. Pemán,et al.  Comparison of the Sensititre YeastOne® Colorimetric Antifungal Panel with the Modified Clinical and Laboratory Standards Institute Broth Microdilution (M38-A) Method for Antifungal Susceptibility Testing of Dermatophytes , 2008, Chemotherapy.

[12]  J. Pemán,et al.  Comparison of E-Test, disk diffusion and a modified CLSI broth microdilution (M 38-A) method for in vitro testing of itraconazole, fluconazole and voriconazole against dermatophytes. , 2008, Medical mycology.

[13]  Clinical,et al.  Reference method for broth dilution antifungal susceptibility testing of filamentous fungi : Approved standard , 2008 .

[14]  Clinical,et al.  Reference method for broth dilution antifungal susceptibility testing of yeasts : Approved standard , 2008 .

[15]  J. Guarro,et al.  In vitro activity of voriconazole against dermatophytes, Scopulariopsis brevicaulis and other opportunistic fungi as agents of onychomycosis. , 2007, International journal of antimicrobial agents.

[16]  D. A. Santos,et al.  In vitro methods for antifungal susceptibility testing of Trichophyton spp. , 2006, Mycological research.

[17]  M. A. Randhawa The effect of dimethyl sulfoxide (DMSO) on the growth of dermatophytes. , 2006, Nihon Ishinkin Gakkai zasshi = Japanese journal of medical mycology.

[18]  M. Ghannoum,et al.  Interlaboratory Study of Quality Control Isolates for a Broth Microdilution Method (Modified CLSI M38-A) for Testing Susceptibilities of Dermatophytes to Antifungals , 2006, Journal of Clinical Microbiology.

[19]  M. Rinaldi,et al.  Antifungal susceptibility testing. , 2006, Infectious disease clinics of North America.

[20]  A. Rattan,et al.  Antifungal susceptibility testing method for resource constrained laboratories. , 2006, Indian journal of medical microbiology.

[21]  J. Guarro,et al.  Effect of Culture Medium on the Disk Diffusion Method for Determining Antifungal Susceptibilities of Dermatophytes , 2006, Antimicrobial Agents and Chemotherapy.

[22]  D. A. Santos,et al.  Evaluation of Broth Microdilution Antifungal Susceptibility Testing Conditions for Trichophyton rubrum , 2005, Journal of Clinical Microbiology.

[23]  M. Pfaller,et al.  Clinical Evaluation of the Sensititre YeastOne Colorimetric Antifungal Plate for Antifungal Susceptibility Testing of the New Triazoles Voriconazole, Posaconazole, and Ravuconazole , 2004, Journal of Clinical Microbiology.

[24]  M. Ghannoum,et al.  Intra- and Interlaboratory Study of a Method for Testing the Antifungal Susceptibilities of Dermatophytes , 2004, Journal of Clinical Microbiology.

[25]  A. Koç,et al.  In vitro susceptibility testing of dermatophytes: comparison of disk diffusion and reference broth dilution methods. , 2004, Diagnostic microbiology and infectious disease.

[26]  J. Guarro,et al.  In Vitro Activities of the New Antifungal Drug Eberconazole and Three Other Topical Agents against 200 Strains of Dermatophytes , 2003, Journal of Clinical Microbiology.

[27]  Aditya K. Gupta,et al.  In vitro susceptibility testing of ciclopirox, terbinafine, ketoconazole and itraconazole against dermatophytes and nondermatophytes, and in vitro evaluation of combination antifungal activity , 2003, The British journal of dermatology.

[28]  J. Guarro,et al.  Interlaboratory evaluation of the Etest for antifungal susceptibility testing of dermatophytes. , 2003, Medical mycology.

[29]  J. Guarro,et al.  Collaborative Evaluation of Optimal Antifungal Susceptibility Testing Conditions for Dermatophytes , 2002, Journal of Clinical Microbiology.

[30]  J. Guarro,et al.  Use of the Sensititre Colorimetric Microdilution Panel for Antifungal Susceptibility Testing of Dermatophytes , 2002, Journal of Clinical Microbiology.

[31]  M. Vaneechoutte,et al.  Susceptibility Testing of Fluconazole by the NCCLS Broth Macrodilution Method, E-Test, and Disk Diffusion for Application in the Routine Laboratory , 2002, Journal of Clinical Microbiology.

[32]  M. Ghannoum,et al.  Antifungal Susceptibility Testing: Practical Aspects and Current Challenges , 2001, Clinical Microbiology Reviews.

[33]  D F Brown,et al.  Quality assurance of antimicrobial susceptibility testing by disc diffusion. , 2001, The Journal of antimicrobial chemotherapy.

[34]  J. Guarro,et al.  In vitro Susceptibility to Itraconazole, Clotrimazole, Ketoconazole and Terbinafine of 100 Isolates of Trichophyton rubrum , 2000, Chemotherapy.

[35]  M. Ghannoum,et al.  Antifungal Susceptibility Testing of Dermatophytes: Establishing a Medium for Inducing Conidial Growth and Evaluation of Susceptibility of Clinical Isolates , 2000, Journal of Clinical Microbiology.

[36]  M. Ghannoum,et al.  Optimal growth conditions for the determination of the antifungal susceptibility of three species of dermatophytes with the use of a microdilution method. , 1999, Journal of the American Academy of Dermatology.

[37]  M. Ghannoum,et al.  Multicenter Comparison of the Sensititre YeastOne Colorimetric Antifungal Panel with the National Committee for Clinical Laboratory Standards M27-A Reference Method for Testing Clinical Isolates of Common and Emerging Candidaspp., Cryptococcus spp., and Other Yeasts and Yeast-Like Organisms , 1999, Journal of Clinical Microbiology.

[38]  M. Ghannoum,et al.  Multisite reproducibility of MIC results by the Sensititre YeastOne colorimetric antifungal susceptibility panel. , 1998, Diagnostic microbiology and infectious disease.

[39]  B. Elewski,et al.  Onychomycosis: Pathogenesis, Diagnosis, and Management , 1998, Clinical Microbiology Reviews.

[40]  M. Klepser,et al.  Evaluation of Endpoints for Antifungal Susceptibility Determinations with LY303366 , 1998, Antimicrobial Agents and Chemotherapy.

[41]  M. Pfaller,et al.  Evaluation of a novel colorimetric broth microdilution method for antifungal susceptibility testing of yeast isolates , 1994, Journal of clinical microbiology.

[42]  J. Galgiani,et al.  Multicenter evaluation of a broth macrodilution antifungal susceptibility test for yeasts , 1993, Antimicrobial Agents and Chemotherapy.

[43]  J. Galgiani,et al.  Collaborative comparison of broth macrodilution and microdilution antifungal susceptibility tests , 1992, Journal of clinical microbiology.

[44]  M. Ghannoum,et al.  Susceptibility testing of Cryptococcus neoformans: a microdilution technique , 1992, Journal of clinical microbiology.

[45]  C. Pérez-Giraldo,et al.  In vitro studies of activities of some antifungal agents against Candida albicans ATCC 10231 by the turbidimetric method , 1992, Antimicrobial Agents and Chemotherapy.

[46]  E. Johnson,et al.  In-vitro resistance to imidazole antifungals in Candida albicans. , 1984, The Journal of antimicrobial chemotherapy.

[47]  T. Granade,et al.  Antimycotic susceptibility testing of dermatophytes in microcultures with a standardized fragmented mycelial inoculum , 1980, Antimicrobial Agents and Chemotherapy.

[48]  E. Bruck,et al.  National Committee for Clinical Laboratory Standards. , 1980, Pediatrics.

[49]  J. Galgiani,et al.  Antimicrobial Susceptibility Testing of Yeasts: a Turbidimetric Technique Independent of Inoculum Size , 1976, Antimicrobial Agents and Chemotherapy.