论文信息 - Detection of oligomerization of Epstein-Barr virus oncoprotein LMP1 by FRET-based probes

Detection of oligomerization of Epstein-Barr virus oncoprotein LMP1 by FRET-based probes

In this study, two FRET-based probes are constructed to research oligomerization of Epstein-Barr virus Oncoprotein LMP1 in live cells. The images of wide-field fluorescence microscopy display that the majority of two LMP1-associated probes co-localized in internal perinuclear membranes. Furthermore, the fluorescence spectra of single cell co-expressed two probes indicated that the ratio of two emission peaks is around one, and the fluorescence spectra changed insignificantly during an hour observation. These findings indicated that LMP1/LMP1 interacted stably in live cells.

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