Isolation, partial characterisation and immunomodulatory activities of polysaccharide from Morchella esculenta.

BACKGROUND Polysaccharides extracted from fungi or algae have shown a variety of medical activities, and the exploitation of polysaccharides for application in pharmacy has been very promising. In this study, the immunomodulatory properties of polysaccharides from Morchella esculenta were investigated to identify immunostimulatory activity and potentially contribute to the therapeutic potential of Morchella esculenta. RESULTS A water-soluble polysaccharide, MEP, was obtained from the fermentation broth of Morchella esculenta. Two fractions of this polysaccharide (MEP-I and MEP-II) were extracted and purified. High-performance gel permeation chromatography analysis showed the average molecular weights of two polysaccharides. Structural properties and compositions of these two fractions were examined by Fourier transform infrared and a high-performance liquid chromatography with an evaporative light scattering detector. Active experiments suggested that the MEP had typical immunostimulatory activity. CONCLUSION These bioactivity tests showed that the polysaccharides from Morchella esculenta presented significant immune modulating activity, and this finding may offer the basis for the popular use of polysaccharides in functional foods or medicine.

[1]  S. Nie,et al.  Isolation, chemical composition and antioxidant activities of a water-soluble polysaccharide from Cyclocarya paliurus (Batal.) Iljinskaja , 2010 .

[2]  Dan-qing Liu,et al.  Antioxidant activity of sulfated polysaccharide fractions extracted from Undaria pinnitafida in vitro. , 2010, International journal of biological macromolecules.

[3]  Fanyun Meng,et al.  Optimization for the production of exopolysaccharides from Morchella esculenta SO-02 in submerged culture and its antioxidant activities in vitro , 2010 .

[4]  Hong-xiang Sun,et al.  Antitumor and immunomodulatory activity of polysaccharides from the roots of Actinidia eriantha. , 2009, Journal of ethnopharmacology.

[5]  Qipeng Yuan,et al.  Isolation, purification and immunobiological activity of a new water-soluble bee pollen polysaccharide from Crataegus pinnatifida Bge. , 2009, Carbohydrate Polymers.

[6]  Jicheng Liu,et al.  Structural elucidation and immunological activity of a polysaccharide from the fruiting body of Armillaria mellea. , 2009, Bioresource technology.

[7]  P. Sun,et al.  Purification and structural elucidation of a novel fucoglucan from the fruiting bodies of Phellinus baumii Pilát. , 2009 .

[8]  Tao Xi,et al.  Immunomodulatory activity of polysaccharides isolated from Strongylocentrotus nudus eggs. , 2008, International immunopharmacology.

[9]  Qingbiao Li,et al.  The isolation and characterization of polysaccharides from longan pulp , 2008 .

[10]  K. Janardhanan,et al.  Aqueous-ethanolic extract of morel mushroom mycelium Morchella esculenta, protects cisplatin and gentamicin induced nephrotoxicity in mice. , 2008, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[11]  Bin Zhang,et al.  Optimization of cultivation conditions for extracellular polysaccharide and mycelium biomass by Morchella esculenta As51620 , 2008 .

[12]  G. Xie,et al.  Immunomodulatory activity of acidic polysaccharides isolated from Tanacetum vulgare L. , 2007, International immunopharmacology.

[13]  Yee‐Shin Lin,et al.  Abrogation of streptococcal pyrogenic exotoxin B-mediated suppression of phagocytosis in U937 cells by Cordyceps sinensis mycelium via production of cytokines. , 2007, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[14]  Q. Mei,et al.  Immunomodulatory activity of polysaccharide isolated from Angelica sinensis. , 2006, International journal of biological macromolecules.

[15]  D. Madamwar,et al.  Partial characterization of extracellular polysaccharides from cyanobacteria. , 2006, Bioresource technology.

[16]  Chi-Hyun Song,et al.  Chemical characteristics and immuno-stimulating properties of biopolymers extracted from Acanthopanax sessiliflorus. , 2006, Journal of biochemistry and molecular biology.

[17]  Moucheng Wu,et al.  Characterization and immunomodulating activities of polysaccharide from Lentinus edodes. , 2005, International immunopharmacology.

[18]  P. Tu,et al.  A study on the immune receptors for polysaccharides from the roots of Astragalus membranaceus, a Chinese medicinal herb. , 2004, Biochemical and biophysical research communications.

[19]  B. Paulsen,et al.  Erratum to “Isolation, partial characterisation and immunomodulating activities of polysaccharides from Vernonia kotschyana Sch. Bip. ex Walp”: [Journal of Ethnopharmacology 91 (2004) 141–152] , 2004 .

[20]  S. Wasser.  Wasser Medicinal mushrooms as a source of antitumor and immunomodulating polysaccharides , 2022 .

[21]  A. Tzianabos.  Polysaccharide immunomodulators as therapeutic agents: structural aspects and biologic function. , 2000, Clinical microbiology reviews.

[22]  A. J. Bolton,et al.  Fractionation and characterization of polysaccharides from abaca fibre , 1998 .

[23]  J. H. Litchfteld,et al.  Nutrient content of morel mushroom mycelium: amino acid composition of the protein. , 1963 .

[24]  C. Nathan,et al.  Nitric oxide and macrophage function. , 1997, Annual review of immunology.